Translational Variations in the Amino Acid Sequence of the α -Chain of Rabbit Hemoglobin
This extract was created in the absence of an abstract.
Excerpt
Transfer experiments in the reticulocyte ribosomal system using separate E. coli transfer RNAs for leucine indicated that one leucine position in the α-chain is different from all the other leucine positions in rabbit hemoglobin. In addition, the amino acid composition of the relevant peptides made us suspect that this leucine position might also be special in its structure (Weisblum, Gonano, von Ehrenstein, and Benzer, 1965).
The experiments reported here were undertaken to determine the position of the exceptional leucine codon in the amino acid sequence of the α-chain. Evidence that this codon is in position 48 will be presented.
As the studies on the amino acid sequence of the α-chain progressed, our suspicion was confirmed that position 48 was not only exceptional in its response to a minor leucine transfer RNA, but also, that leucine was not the only amino acid occupying that position. In the α-chain from a single...
