Imaging of Mitochondrial and Nuclear Ca2+ Dynamics in Arabidopsis Roots

Giovanna Loro; Alex Costa

doi:10.1101/pdb.prot073049

Protocol

Imaging of Mitochondrial and Nuclear Ca²⁺ Dynamics in Arabidopsis Roots

Giovanna Loro1,2 and
Alex Costa2,3

¹Department of Biology, University of Padova, 35131 Padova, Italy;
²Department of Biosciences, University of Milan, 20133 Milan, Italy

Abstract

Here we report a method for analyzing mitochondrial Ca²⁺ dynamics in plant root cells by means of the newly generated cameleon probe 4mt-YC3.6. The use of plants expressing both nuclear- and mitochondrial-targeted cameleon, along with the resolution of confocal laser scanning microscopy (CLSM), allow simultaneous recordings of mitochondrial and nuclear Ca²⁺ dynamics within the same cell.