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Volume 167, Issue 7

The role of -arabinose metabolism for O157:H7 in edible plants Open Access

This article is part of the Environmental Sensing and Cell-Cell Communication collection

Abstract

Arabinose is a major plant aldopentose in the form of arabinans complexed in cell wall polysaccharides or glycoproteins (AGP), but comparatively rare as a monosaccharide. -arabinose is an important bacterial metabolite, accessed by pectolytic micro-organisms such as via pectin and hemicellulose degrading enzymes. However, not all plant-associated microbes encode cell-wall-degrading enzymes, yet can metabolize -arabinose, raising questions about their use of and access to the glycan in plants. Therefore, we examined -arabinose metabolism in the food-borne pathogen O157:H7 (isolate Sakai) during its colonization of plants. -arabinose metabolism () and transport () genes were activated at 18 °C by -arabinose and expressed over prolonged periods . Although deletion of did not impact the colonization ability of O157:H7 (Sakai) on spinach and lettuce plants (both associated with STEC outbreaks), was induced on exposure to spinach cell-wall polysaccharides. Furthermore, debranched and arabinan oligosaccharides induced metabolism gene expression , and stimulated modest proliferation, while immobilized pectin did not. Thus, O157:H7 (Sakai) can utilize pectin/AGP-derived -arabinose as a metabolite. Furthermore, it differs fundamentally in gene organization, transport and regulation from the related pectinolytic species , reflective of distinct plant-associated lifestyles.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bacterial pathogens , food safety , plant cell wall degrading enzymes , plant-microbe interactions and vegetables
Funding
This study was supported by the:
  • Leverhulme Trust (Award RPG-096)
    • Principle Award Recipient: NicolaJean Holden
  • University of Reading (Award PhD)
    • Principle Award Recipient: RobertWilson Jackson
  • Rural and Environment Science and Analytical Services Division (Award RD2.3.3)
    • Principle Award Recipient: NicolaJean Holden
  • Rural and Environment Science and Analytical Services Division (Award RD3.1.3)
    • Principle Award Recipient: NicolaJean Holden
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-07-28
2024-04-26
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The role of l-arabinose metabolism for Escherichia coli O157:H7 in edible plants
Microbiology 167, 001070 (2021); https://doi.org/10.1099/mic.0.001070
/content/journal/micro/10.1099/mic.0.001070
/content/journal/micro/10.1099/mic.0.001070
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