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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

sp. nov., sp. nov. and sp. nov., isolated from the Qinghai–Tibet Plateau No Access

Abstract

Six Gram-stain-positive, aerobic and irregular-rod-shaped actinobacteria (ZJ1313, ZJ1307, MC1495, Y192, 603 and X2025) were isolated from the Qinghai–Tibet Plateau of China and were characterized using a polyphasic taxonomic method. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the six new strains formed three distinct clusters within the genus , and strains ZJ1313 and ZJ1307 were most closely related to JCM 31492 (16S rRNA gene sequence similarity, 98.0 %), MC1495 and Y192 to 78 (98.5 %), and 603 and X2025 to JCM 14815 (97.6 %). The digital DNA–DNA hybridization values of strains ZJ1313, MC1495 and 603 among each other and with type strains of their closest relatives were all below the 70 % cut-off point, but values within each pair of new strains were all higher than the threshold. The major fatty acids of these strains were iso-C, C 8 or C 9. MK-8(H) was the predominant respiratory menaquinone and ʟʟ−2,6-diaminopimelic acid was the diagnostic diamino acid. All the strains shared diphosphatidylglycerol (predominant), phosphatidylglycerol, phosphatidylcholine and phosphatidylinositol as the common polar lipids, with minor difference in the types of unidentified phospholipids, glycolipids and lipids. The G+C contents based on genomic DNA of strains ZJ1313, MC1495 and 603 were 72.5, 72.1 and 73.2 mol%, respectively. The above results suggested that strain pairs ZJ1313/ZJ1307, MC1495/Y192 and 603/X2025 represent three new species of genus , for which the names sp. nov. (ZJ1313=GDMCC 4.177=KCTC 49537=JCM 34185), sp. nov. (MC1495=GDMCC 4.176=KCTC 49536=JCM 34307) and sp. nov. (603=CGMCC 4.7510=DSM 106494) are proposed accordingly.

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Keyword(s): Nocardioides campestrisoli , Nocardioides ochotonae , Nocardioides pantholopis , Ochotona curzoniae , Pantholops hodgsonii and Pseudois nayaur
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2022-10-07
2024-04-25
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Nocardioides ochotonae sp. nov., Nocardioides campestrisoli sp. nov. and Nocardioides pantholopis sp. nov., isolated from the Qinghai–Tibet Plateau
Int J Syst Evol Microbiol 72, 005507 (2022); https://doi.org/10.1099/ijsem.0.005507
/content/journal/ijsem/10.1099/ijsem.0.005507
/content/journal/ijsem/10.1099/ijsem.0.005507
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