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Volume 71, Issue 5

gen. nov., sp. nov., a member of a lineage of freshwater bacteria with small genome sizes No Access

Abstract

The actinobacterial strain 15G-AUS-rot was isolated from an artificial pond located near Salzburg, Austria. The strain showed 16S rRNA gene sequence similarities of 98.7 % to Aquiluna rubra and of 96.6 and 96.7 % to the two validly described species of the genus . Phylogenetic reconstructions based on 16S rRNA gene sequences and genome-based on amino acid sequences of 118 single copy genes referred strain 15G-AUS-rot to the family and therein to the so-called subcluster Luna-1. The genome-based phylogenetic tree showed that the new strain represents a putative new genus. Cultures of strain 15G-AUS-rot were light red pigmented and comprised very small, rod-shaped cells. They metabolized a broad variety of substrates. Major fatty acids (>10 %) of cells were iso-C, antiso-C and iso-C. The major respiratory quinone was MK-11 and a minor component was MK-10. The peptidoglycan structure belonged to an unusual B type. The closed genome sequence of the strain was very small (1.4 Mbp) and had a DNA G+C content of 54.8 mol%. An interesting feature was the presence of genes putatively encoding the complete light-driven proton pumping actinorhodopsin/retinal system, which were located at three different positions of the genome. Based on the characteristics of the strain, a new genus and a new species termed is proposed for strain 15G-AUS-rot (=DSM 107803=JCM 32974).

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Keyword(s): Aquiluna , freshwater , genome , genome size and Microbacteriaceae
© 2021 The Authors
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2021-05-17
2024-04-24
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Aquiluna borgnonia gen. nov., sp. nov., a member of a Microbacteriaceae lineage of freshwater bacteria with small genome sizes
Int J Syst Evol Microbiol 71, 004825 (2021); https://doi.org/10.1099/ijsem.0.004825
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