1887

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Volume 68, Issue 6

sp. nov., isolated from the ciliate and emendation of the genus Free

Abstract

A Gram-stain-negative, oxidase-negative and catalase-negative, motile, rod-shaped bacterial strain, designated PCS8, hosted by the ciliate was investigated by using a polyphasic approach. Strain PCS8 was observed to be able to grow at 12–44 °C (optimum, 36–37 °C), at pH 6.0–10.0 (optimum, 7.0) and in the presence of 0–3 % NaCl (optimum, 1–2 %). It could hydrolyse starch and aesculin and produce acid from -sorbitol, -inositol, glycerol and -rhamnose. The sequence similarity of the new isolate was 96.9 % with respect to and 96.3 % with respect to . Phylogenetically, strain PCS8 falls within the cluster comprising the species. The predominant cellular fatty acids of strain PCS8 were C (35.8 %), summed feature 3 (Cω7 and/or Cω6; 35.1 %) and summed feature 8 (Cω7 and/or Cω6; 10.8 %). This novel strain also contained various fatty acids that are not detected in other members of the genus , such as C 3-OH, Cω9 and summed feature 5 (Cω6,9 and/or C ante). Strain PCS8 contained ubiquinone-8 as the sole respiratory quinone and phosphatidylethanolamine and phosphatidylglycerol as major polar lipids. The G+C content of the genomic DNA of the type strain was 66.5 mol%. Based on the distinct phenotypic, phylogenetic, chemotaxonomic and G+C content results, strain PCS8 represents a currently undescribed species within the genus in the family , for which we suggest the name sp. nov. with the type strain PCS8 (=KCTC 62038=JCM 32226). An emended description of the genus is also provided.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ciliate-host , new species , Paramecium caudatum and Pseudaeromonas re-definition
© 2018 IUMS
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2018-06-01
2024-04-16
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Pseudaeromonas paramecii sp. nov., isolated from the ciliate Paramecium caudatum and emendation of the genus Pseudaeromonas
Int J Syst Evol Microbiol 68, 1845 (2018); https://doi.org/10.1099/ijsem.0.002746
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