1887

Abstract

A polyphasic taxonomic study was undertaken to clarify the exact position of type strain HEL-45 of Wagner-Döbler . 2004. A combination of physiological properties of HEL-45 were similar to those of its closest relatives, KMM 3584 and KMM 3554. The results of 16S rRNA gene sequences analysis indicated that HEL-45 and the two relatives shared 99.6 % similarity and formed a coherent cluster in the phylogenetic tree. The close relationship between HEL-45 and the two relatives was underpinned by the results for chemotaxonomic characters, including the fatty acids, quinone and polar lipids. The digital DNA–DNA hybridization values and between HEL-45 and the two relatives were 25.9 and 31.6 %, respectively, which were well below the 70 % threshold value recommended for delineation of bacterial species. The comparative phenotypic and genotypic analyses supported the incorporation of into the genus as comb. nov.; the type strain is HEL-45 (=MCCC 1A11773=DSM 14862=NCIMB 13983).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.001950
2017-07-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/67/7/2328.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.001950&mimeType=html&fmt=ahah

References

  1. Wagner-Döbler I, Rheims H, Felske A, El-Ghezal A, Flade-Schroder D et al. Oceanibulbus indolifex gen. nov., sp. nov., a North Sea alphaproteobacterium that produces bioactive metabolites. Int J Syst Evol Microbiol 2004; 54:1177–1184 [CrossRef]
    [Google Scholar]
  2. Sorokin DY. Sulfitobacter pontiacus gen. nov., sp. nov. a new heterotrophic bacterium from the Black Sea, specialized on sulfite oxidation. Mikrobiologiya 1995; 64:354–365
    [Google Scholar]
  3. Kumari P, Bhattacharjee S, Poddar A, Das SK. Sulfitobacter faviae sp. nov., isolated from the coral Favia veroni . Int J Syst Evol Microbiol 2016; 66:3786–3792 [View Article][PubMed]
    [Google Scholar]
  4. Ivanova EP, Gorshkova NM, Sawabe T, Zhukova NV, Hayashi K et al. Sulfitobacter delicatus sp. nov. and Sulfitobacter dubius sp. nov., respectively from a starfish (Stellaster equestris) and sea grass (Zostera marina). Int J Syst Evol Microbiol 2004; 54:475–480 [View Article][PubMed]
    [Google Scholar]
  5. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article][PubMed]
    [Google Scholar]
  6. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425[PubMed]
    [Google Scholar]
  7. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985783–791 [CrossRef]
    [Google Scholar]
  8. Yoon JH, Kang SJ, Lee MH, Oh TK. Description of Sulfitobacter donghicola sp. nov., isolated from seawater of the East Sea in Korea, transfer of Staleya guttiformis Labrenz, et al. 2000 to the genus Sulfitobacter as Sulfitobacter guttiformis comb. nov. and emended description of the genus Sulfitobacter . Int J Syst Evol Microbiol 2007; 57:1788–1792 [CrossRef]
    [Google Scholar]
  9. Brosius J, Palmer ML, Kennedy PJ, Noller HF. Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli . Proc Natl Acad Sci USA 1978; 75:4801–4805 [View Article][PubMed]
    [Google Scholar]
  10. Wayne L, Brenner D, Colwell R, Grimont P, Kandler O et al. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 1987; 37:463–464 [CrossRef]
    [Google Scholar]
  11. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.001950
Loading
/content/journal/ijsem/10.1099/ijsem.0.001950
Loading

Data & Media loading...

Supplements

Supplementary File 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error