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Volume 65, Issue Pt_11

sp. nov., isolated from Pu'er tea Free

Abstract

A novel Gram-staining-positive, aerobic, endospore-forming, rod-shaped bacterial strain, YN2, was isolated from ripened Pu'er tea. Phylogenetic analysis of 16S rRNA gene sequences showed that the strain represented a novel species of the genus . The strains most closely related to strain YN2 were JCM 18268 and JCM 16352, with 16S rRNA similarities of 98.6 and 95.5 %, respectively. Chemotaxonomic data supported the affiliation of the new isolate to the genus , including MK-7 as the major menaquinone, DNA G+C content of 51 mol%, cell-wall type A1γ (-diaminopimelic acid as the diagnostic diamino acid) and anteiso-C and iso-C as the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylethanolamine and phospholipid. Strain YN2 could be differentiated from recognized species of the genus based on phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from this polyphasic study, sp. nov., is proposed, with strain YN2 ( = CGMCC 1.12968 = JCM 30953) as the type strain.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 1308085MC31)
  • Anhui Provincial Natural Science Foundation
© 2015 IUMS

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sp. nov., isolated from Pu’er tea
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2015-11-01
2024-04-24
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Paenibacillus yunnanensis sp. nov., isolated from Pu'er tea
Int J Syst Evol Microbiol 65, 3806 (2015); https://doi.org/10.1099/ijsem.0.000496
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