1887

Abstract

A Gram-stain-negative, aerobic, non-flagellated and rod-shaped or ovoid bacterial strain, designated GJSW-36, was isolated from seawater at Geoje island in the South Sea, South Korea. Strain GJSW-36 grew optimally at pH 7.0–8.0, at 25 °C and in the presence of 2 % (w/v) NaCl. A neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain GJSW-36 fell within the clade comprising the type strains of species of the genus and . Strain GJSW-36 exhibited 16S rRNA gene sequence similarity values of 94.2–96.0 % to the type strains of species of the genus and and of 93.8–94.5 % to the type strains of the other species of the genus . Strain GJSW-36 contained ubiquinone-8 (Q-8) as the predominant ubiquinone and summed feature 3 (Cω7 and/or Cω6), Cω8 and C as the major fatty acids. The major polar lipids of strain GJSW-36 were phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content of strain GJSW-36 was 45.1 mol%. Differential phenotypic properties, together with the phylogenetic distinctiveness, demonstrated that strain GJSW-36 is separated from species of the genus and . On the basis of the data presented, strain GJSW-36 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GJSW-36 ( = KCTC 42155 = CECT 8656). From this study, it is also proposed that should be reclassified as a member of the genus and the description of the genus is emended.

Funding
This study was supported by the:
  • Program for Collection, Management and Utilization of Biological Resources
  • Ministry of Science, ICT & Future Planning (MSIP) of the Republic of Korea (Award grant NRF-2013M3A9A5075953)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.067611-0
2014-11-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/11/3676.html?itemId=/content/journal/ijsem/10.1099/ijs.0.067611-0&mimeType=html&fmt=ahah

References

  1. Barrow G. I., Feltham R. K. A. ( 1993 ). Cowan and Steel’s Manual for the Identification of Medical Bacteria, , 3rd edn.. Cambridge:: Cambridge University Press;. [View Article]
    [Google Scholar]
  2. Baumann P., Baumann L. ( 1981 ). The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . . In The Prokaryotes, pp. 13021331. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . Berlin:: Springer;.
    [Google Scholar]
  3. Bowman J. P., McMeekin T. A. ( 2005 ). Order X. Alteromonadales ord. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, part B, pp. 443490. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;. [View Article]
    [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. ( 2001 ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51, 19972006. [View Article] [PubMed]
    [Google Scholar]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. ( 1957 ). Kinetic studies of pigment synthesis by nonsulfur purple bacteria. . J Cell Comp Physiol 49, 2568. [View Article]
    [Google Scholar]
  6. Embley T. M., Wait R. ( 1994 ). Structural lipids of eubacteria. . In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics, pp. 121161. Edited by Goodfellow M., O’Donnell A. G. . Chichester:: Wiley;.
    [Google Scholar]
  7. Hosoya S., Adachi K., Kasai H. ( 2009 ). Thalassomonas actiniarum sp. nov. and Thalassomonas haliotis sp. nov., isolated from marine animals. . Int J Syst Evol Microbiol 59, 686690. [View Article] [PubMed]
    [Google Scholar]
  8. Jean W. D., Shieh W. Y., Liu T. Y. ( 2006 ). Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas . . Int J Syst Evol Microbiol 56, 12451250. [View Article] [PubMed]
    [Google Scholar]
  9. Jung Y.-T., Park S., Yoon J.-H. ( 2014 ). Thalassomonas fusca sp. nov., a novel gammaproteobacterium isolated from tidal flat sediment. . Antonie van Leeuwenhoek 105, 8187. [View Article] [PubMed]
    [Google Scholar]
  10. Komagata K., Suzuki K.-I. ( 1987 ). Lipid and cell wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  11. Lányí B. ( 1987 ). Classical and rapid identification methods for medically important bacteria. . Methods Microbiol 19, 167. [View Article]
    [Google Scholar]
  12. Macián M. C., Ludwig W., Schleifer K. H., Garay E., Pujalte M. J. ( 2001 ). Thalassomonas viridans gen. nov., sp. nov., a novel marine γ-proteobacterium. . Int J Syst Evol Microbiol 51, 12831289.[PubMed] [CrossRef]
    [Google Scholar]
  13. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  14. Park S., Choi W.-C., Oh T.-K., Yoon J.-H. ( 2011 ). Thalassomonas agariperforans sp. nov., an agarolytic bacterium isolated from marine sand. . Int J Syst Evol Microbiol 61, 25732576. [View Article] [PubMed]
    [Google Scholar]
  15. Park S., Park D.-S., Bae K. S., Yoon J.-H. ( 2014 ). Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. . Int J Syst Evol Microbiol 64, 13781383. [View Article] [PubMed]
    [Google Scholar]
  16. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  18. Staley J. T. ( 1968 ). Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. . J Bacteriol 95, 19211942.[PubMed]
    [Google Scholar]
  19. Tamaoka J., Komagata K. ( 1984 ). Determination of DNA base composition by reverse-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25, 125128. [View Article]
    [Google Scholar]
  20. Thompson F. L., Barash Y., Sawabe T., Sharon G., Swings J., Rosenberg E. ( 2006 ). Thalassomonas loyana sp. nov., a causative agent of the white plague-like disease of corals on the Eilat coral reef. . Int J Syst Evol Microbiol 56, 365368. [View Article] [PubMed]
    [Google Scholar]
  21. Yi H., Bae K. S., Chun J. ( 2004 ). Thalassomonas ganghwensis sp. nov., isolated from tidal flat sediment. . Int J Syst Evol Microbiol 54, 377380. [View Article] [PubMed]
    [Google Scholar]
  22. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. ( 1996 ). Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. . Int J Syst Bacteriol 46, 502505. [View Article]
    [Google Scholar]
  23. Yoon J.-H., Lee S. T., Park Y.-H. ( 1998 ). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. . Int J Syst Bacteriol 48, 187194. [View Article] [PubMed]
    [Google Scholar]
  24. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. ( 2003 ). Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. . Int J Syst Evol Microbiol 53, 5357. [View Article] [PubMed]
    [Google Scholar]
  25. Zhang Y., Tang K., Shi X., Zhang X.-H. ( 2014 ). Description of Thalassotalea piscium gen. nov., sp. nov., isolated from flounder (Paralichthys olivaceus), reclassification of four species of the genus Thalassomonas as members of the genus Thalassotalea gen. nov. and emended description of the genus Thalassomonas . . Int J Syst Evol Microbiol 64, 12231228. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.067611-0
Loading
/content/journal/ijsem/10.1099/ijs.0.067611-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error