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Abstract

Macro-algae are a good source of agar oligosaccharides, which can be obtained through bacterial enzymatic hydrolysis. The agarase enzyme secreted by the micro-organisms cleaves the cell wall of the algae and releases agar oligosaccharides as degradation products with various applications. Agarolytic bacteria were isolated from the marine algae sp., and sp., and studied for their agar-degrading properties. Among the 70 isolates, 2 isolates (A13 and Sg8) showed agarase activity in assays. The maximum agarolytic index was recorded in the isolate Sg8 (3.75 mm and 4.29 µg ml agarase activity), followed by the isolate A13 (2.53 mm and 2.6 µg ml agarase activity). Optimum agarase production of isolate Sg8 was observed at pH7 and at a temperature of 25 °C in 24–48 h, whereas for isolate A13 the optimum production was at pH7 and at a temperature of 37 °C in 48 h. The identities of the agarolytic isolates (Sg8 and A13) were confirmed based on microscopy, morphological, biochemical and molecular analysis as [National Center for Biotechnology Information (NCBI) GenBank accession number MK121204.1] and [NCBI GenBank accession number MK825484.1], respectively.

Funding
This study was supported by the:
  • BIRAC (Award BT/SBIRI/1394/31/16)
    • Principle Award Recipient: Latha K
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2020-09-10
2024-04-23
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References

  1. Araki C, Arai K. Studies on the chemical constitution of agar-agar. XVIII. Isolation of a new crystalline disaccharide by enzymatic hydrolysis of agar-agar. Bull Chem Soc Jpn 1956; 29:339–345 [View Article]
    [Google Scholar]
  2. Kawaroe M, Pratiwi I, Sunudin A. Isolation and characterization of marine bacteria from macroalgae Gracilaria salicornia and Gelidium latifolium on agarolitic activity for bioethanol production. IOP Conf Ser Earth Environ Sci 2017; 65:012025 [View Article]
    [Google Scholar]
  3. Hodgson DA, Chater KF. A chromosomal locus controlling extracellular agarase production by Streptomyces coelicolor A3(2), and its inactivation by chromosomal integration of plasmid SCP1. J Gen Microbiol 1981; 124:339–348
    [Google Scholar]
  4. Fu XT, Kim SM. Agarase: review of major sources, categories, purification method, enzyme characteristics and applications. Mar Drugs 2010; 8:200–218 [View Article][PubMed]
    [Google Scholar]
  5. Waksman SA, Bavendamm W. On the decomposition of agar-agar by an aerobic bacterium. J Bacteriol 1931; 22:91–102 [View Article][PubMed]
    [Google Scholar]
  6. Goresline HE. Studies of agar-digesting bacteria. J Bacteriol 1933; 26:435–457 [View Article][PubMed]
    [Google Scholar]
  7. Sugano Y, Terada I, Arita M, Noma M, Matsumoto T. Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107. Appl Environ Microbiol 1993; 59:1549–1554 [View Article][PubMed]
    [Google Scholar]
  8. Fukasawa S, Dunlap PV, Baba M, Osumi M. Identification of an agar-digesting, luminous bacterium. Agric Biol Chem 1987; 51:265–268 [View Article]
    [Google Scholar]
  9. Kim B-C, Poo H, Lee KH, Kim MN, Park D-S et al. Simiduia areninigrae sp. nov., an agarolytic bacterium isolated from sea sand. Int J Syst Evol Microbiol 2012; 62:906–911 [View Article][PubMed]
    [Google Scholar]
  10. Romanenko LA, Zhukova NV, Rohde M, Lysenko AM, Mikhailov VV et al. Pseudoalteromonas agarivorans sp. nov., a novel marine agarolytic bacterium. Int J Syst Evol Microbiol 2003; 53:125–131 [View Article][PubMed]
    [Google Scholar]
  11. Sie Y-F, Yang H-C, Lee Y. The discovery of agarolytic bacterium with agarase gene containing plasmid, and some enzymology characteristics. Int J Appl Sci Eng 2009; 7:25–41
    [Google Scholar]
  12. Lakshmikanth M, Manohar S, Patnakar J, Vaishampayan P, Shouche Y et al. Optimization of culture conditions for the production of extracellular agarases from newly isolated Pseudomonas aeruginosa AG LSL-11. World J Microbiol Biotechnol 2006; 22:531–537 [View Article]
    [Google Scholar]
  13. Jean WD, Shieh WY, Liu TY. Thalassomonas agarivorans sp. nov., a marine agarolytic bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the genus Thalassomonas . Int J Syst Evol Microbiol 2006; 56:1245–1250 [View Article][PubMed]
    [Google Scholar]
  14. Leon O, Quintana L, Peruzzo G, Slebe JC. Purification and properties of an extracellular agarase from Alteromonas sp. strain C-1. Appl Environ Microbiol 1992; 58:4060–4063 [View Article][PubMed]
    [Google Scholar]
  15. Xavier Chiura H, Kita-Tsukamoto K. Purification and characterisation of a novel agarase secreted by a marine bacterium, Pseudoalteromonas sp. strain CKT1. Microbes Environ 2000; 15:11–22 [View Article]
    [Google Scholar]
  16. Zhong Z, Toukdarian A, Helinski D, Knauf V, Sykes S et al. Sequence analysis of a 101-kilobase plasmid required for agar degradation by a Microscilla isolate. Appl Environ Microbiol 2001; 67:5771–5779 [View Article][PubMed]
    [Google Scholar]
  17. Ohta Y, Hatada Y, Nogi Y, Li Z, Ito S et al. Cloning, expression, and characterization of a glycoside hydrolase family 86 β-agarase from a deep-sea Microbulbifer-like isolate. Appl Microbiol Biotechnol 2004; 66:266–275 [View Article][PubMed]
    [Google Scholar]
  18. Yeong H-Y, Khalid N, Phang S-M. Protoplast isolation and regeneration from Gracilaria changii (Gracilariales, Rhodophyta). J Appl Phycol 2008; 20:641–651 [View Article]
    [Google Scholar]
  19. Kong JY. Production and functional properties of agarooligosaccharides. Proceedings of Symposium on Scientific Study and Industrialization of Health Food Taipai: Health Food Society of Taiwan; 2001 pp 60–81
    [Google Scholar]
  20. Srinivasan R, Sengali Ragunath K, Karuppiah V, Radhesh Krishnan S, Gracy M et al. Isolation and screening of seaweed associated microbes for development of marine based agri – inputs. Seaweed Res Utiln 2017; 39:39–46
    [Google Scholar]
  21. Jannasch HW. Enrichments of aquatic bacteria in continuous culture. Archiv Mikrobiol 1967; 59:165–173 [View Article]
    [Google Scholar]
  22. Miller GL, Blum R, Glennon WE, Burton AL. Measurement of carboxymethylcellulase activity. Anal Biochem 1960; 1:127–132 [View Article]
    [Google Scholar]
  23. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG et al. Current Protocols in Molecular Biology New York: Wiley; 1988 pp 241–242
    [Google Scholar]
  24. Sun FJ, Harish A, Caetano-Anollés G. Phylogenetic utility of RNA structure: evolution’s arrow and emergence of early biochemistry and diversified life. In Caetano-Anollés G. ed Evolutionary Bioinformatics and Systems Biology Hoboken, NJ: Wiley–Blackwell; 2010 pp 329–360
    [Google Scholar]
  25. Kloareg B, Quatrano RS. Structure of the cell walls of marine algae and ecophysiological functions of the matrix polysaccharides. Oceanogr Mar Biol Ann Rev 1988.; 26:259–315
    [Google Scholar]
  26. Rajeswari S, Jaiganesh R, Muthukumar R, Jaganathan MK. Isolation and characterization of an agarase producing bacteria from marine sediment. Int J Chemtech Res 2016; 9:437–446
    [Google Scholar]
  27. Hu Z, Lin B-K, Xu Y, Zhong MQ, Liu G-M. Production and purification of agarase from a marine agarolytic bacterium agarivorans sp. HZ105. J Appl Microbiol 2009; 106:181–190 [View Article][PubMed]
    [Google Scholar]
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