1887

Abstract

SUMMARY: The genetic properties of transformable (SR 5a) and non-transformable (SR30) streptomycin-resistant streptococci were examined. In mutant SR30, the very high resistance (VHR) phenotype appeared to be due to at least two factors; a high resistance (HR) mutation and an enhancing modifier gene. In mutant SR 5a, resistance was due to a single VHR mutation which is allelic to the HR mutation of mutant SR 30. The transfer of non-trans-formability (Ntr) by SR 30 DNA revealed that this factor is separable from the streptomycin resistance marker and the enhancing modifier. Results indicate co-transfer of Ntr with the HR marker.

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/content/journal/micro/10.1099/00221287-65-2-139
1971-02-01
2024-04-25
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References

  1. Bryan B. E. 1961; Genetic modifiers of streptomycin resistance in Pneumococcus.. Journal of Bacteriology 82:461–470
    [Google Scholar]
  2. Ephrati-Elizur E. 1965; Resistance to actinomycin D and transformability in B. subtilis. . Biochemical and Biophysical Research Communications 18:103–107
    [Google Scholar]
  3. Fox M. S., Hotchkiss R. D. 1957; Initiation of bacterial transformation.. Nature, London 179:1322–1325
    [Google Scholar]
  4. Goodgal S. H. 1961; Studies on transformation of Haemophilus influenzae. IV. Linked and unlinked transformations.. Journal of General Physiology 45:205–228
    [Google Scholar]
  5. Lederberg J., Lederberg E. M. 1952; Replica plating and indirect selection of bacterial mutants.. Journal of Bacteriology 63:399–406
    [Google Scholar]
  6. Okubo S., Romig W. R. 1966; Impaired transformability of Bacillus subtilis mutant sensitive to mitomycin C and ultraviolet radiation.. Journal of Molecular Biology 15:440–454
    [Google Scholar]
  7. Pakula R., Walczak W. 1963; On the nature of competence of transformable streptococci.. Journal of General Microbiology 31:125–133
    [Google Scholar]
  8. Perry D. 1968; Transformability of streptomycin-resistant group H streptococci.. Journal of Bacteriology 95:132–138
    [Google Scholar]
  9. Ravin A. W., DeSa J. D. H. 1964; Genetic linkage of mutational sites affecting similar characters in Pneumococcus and Streptococcus.. Journal of Bacteriology 87:86–96
    [Google Scholar]
  10. Ravin A. W., Mishra A. K. 1965; Relative frequencies of different kinds of spontaneous and induced mutants of pneumococci and streptococci capable of growth in the presence of streptomycin.. Journal of Bacteriology 90:1161–1173
    [Google Scholar]
  11. Rotheim M. B., Ravin A. W. 1961; The mapping of genetic loci affecting streptomycin resistance in Pneumococcus.. Genetics 46:1619–1634
    [Google Scholar]
  12. Sirotnak F. M., Lunt R. B., Hutchison D. J. 1963; Alteration in transformability of Diplococcus pneumoniae after the acquisition of genetic determinants inducing resistance to erythromycin.. Journal of Bacteriology 86:735–739
    [Google Scholar]
  13. Young F. E., Spizizen J. 1961; Physiological and genetic factors affecting transformation of Bacillus subtilis. . Journal of Bacteriology 81:823–829
    [Google Scholar]
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