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Volume 147, Issue 2

The operon of B52: a molecular analysis of metalloprotease and lipase production

The GenBank accession numbers for the sequences reported in this paper are AF216700, AF216701 and AF216702.

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Abstract

Extracellular protease and lipase production by psychrotrophic strains of is repressed by iron and regulated by temperature. The regulation of protease and lipase has been investigated in B52. Whereas lipase production is increased below the optimum growth temperature (‘low-temperature regulation’), protease production was relatively constant and only decreased above the optimum growth temperature. The genes encoding protease () and lipase () are encoded at opposite ends of a contiguous set of genes which also includes protease inhibitor, Type I secretion functions and two autotransporter proteins. Evidence is presented indicating that these genes constitute an operon, with a promoter adjacent to which has been identified by S1 nuclease analysis. The regulation of and has been investigated at the RNA level and using fusion strains. Whereas the data are consistent with iron regulation at the transcriptional level, a fusion is not regulated by temperature, suggesting that temperature regulation is post-transcriptional or post-translational. The possibility of regulation at the level of mRNA decay is discussed.

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Keyword(s): ECF, extracytoplasmic function , gene regulation , lipase , promoter , Protease and Pseudomonas fluorescens
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2001-02-01
2024-04-24
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The aprX–lipA operon of Pseudomonas fluorescens B52: a molecular analysis of metalloprotease and lipase productionThe GenBank accession numbers for the sequences reported in this paper are AF216700, AF216701 and AF216702.
Microbiology 147, 345 (2001); https://doi.org/10.1099/00221287-147-2-345
/content/journal/micro/10.1099/00221287-147-2-345
/content/journal/micro/10.1099/00221287-147-2-345
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