1887

Abstract

Phage D10, a O-1 E1 Tor group X phage, is one of the five newly isolated phages used in the phage typing scheme developed for O-1 biotype E1 Tor and belongs to the Myoviridae family. From electron microscopic studies it is shown that phage D10 has a DNA genome of 32±0.2 kb. This is the first report where it has been shown by the construction of a partial denaturation map that this vibriophage genome is nonpermuted and has cohesive ends. The location of the ends of the DNA in the phage head has also been inferred.

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/content/journal/jgv/10.1099/0022-1317-74-12-2749
1993-12-01
2024-04-23
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References

  1. Adams M. H. 1959 Bacteriophages New York: Interscience Publishers;
    [Google Scholar]
  2. Ansari M. Q., Pal S. C. 1990; Phage typing of Vibrio cholerae O1 biotype El Tor strains. Indian Journal of Medical Research 91:263–265
    [Google Scholar]
  3. Basu S., Mukerjee S. 1968; Bacteriophage typing of Vibrio El Tor. Experientia 24:299–300
    [Google Scholar]
  4. Chattopadhyay D. J., Sarkar B. L., Ansari M. Q., Chakrabarti B. K., Roy M. K., Ghosh A. N., Pal S. C. 1993; New phage typing scheme for Vibrio cholerae O-1 biotype El Tor strains. Journal of Clinical Microbiology 31:1579–1585
    [Google Scholar]
  5. Chattoraj D. K., Inman R. B. 1974; Location of DNA ends in P2, 186, P4 and lambda bacteriophage heads. Journal of Molecular Biology 87:11–22
    [Google Scholar]
  6. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymologv 21:413–428
    [Google Scholar]
  7. Ghosh A. N., Ansari M. Q., Datta G. C. 1989; Isolation and morphological characterization of El Tor cholera phages. Journal of General Virology 70:2241–2243
    [Google Scholar]
  8. Inman R. B. 1982; Partial denaturation mapping of DNA determined by electron microscopy. In Electron Microscopy in Biology vol 2 pp 237–271 Edited by Griffith J. D. New York: John Wiley and Sons;
    [Google Scholar]
  9. Kleinschmidt A. K., Lang D., Jacherts D., Zahn R. K. 1962; Darstelkeng und Längenmessungen des gesamten Desoxyribonuclin- saure-inhultes von T2-Bakteriophagen. Biochimica et biophvsica acta 61:857–864
    [Google Scholar]
  10. Mukerjee S. 1978; Principles and practice of typing Vibrio cholerae. Methods in Microbiology 12:50–115
    [Google Scholar]
  11. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: A Laboratory Manual 2nd edn New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Tye B. K., Huberman J. A., Botstein D. 1974; Non-random circular permutation of phage P22 DNA. Journal of Molecular Biology 85:501–532
    [Google Scholar]
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