1887

Abstract

Summary

The ability of respiratory syncytial virus (RSV) and parainfluenza virus type 3 (PIV3) to replicate in peripheral blood monocytes cultured for 1, 2, 4 or 7 days prior to infection was investigated. Inoculation of 1-day old monocytes produced at least tenfold less new virus than infection of the older, more macrophage-like cells for both viruses. PIV3 induced extensive syncytium formation, whereas RSV caused a cytopathic effect manifest by increased rounding of the cells with minimal syncytium formation. Supernatants of infected monocytes were assayed for human interferon-a (HuIFN-) in an attempt to explain the restricted viral replication in the youngest monocytes. In PIV3-infected cells, HuIFN- production was inversely correlated with new virus formation. Monocytes infected after 1 day in culture produced 800 IU/ml of HuIFN-; the older cells produced 100 to 200 IU/ml. In contrast, monocytes infected on day 1 with RSV produced minimal amounts (1·5 IU/ml) of HuIFN-. Increasing amounts of HuIFN- were detected in cells infected with RSV after 2, 4 or 7 days in culture, reaching a maximum of 400 IU/ml on day 7. Further investigation of the apparent restriction of replication in young monocyte cultures may be helpful in understanding the pathogenesis of these respiratory infections.

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1987-06-01
2024-03-28
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References

  1. Arbeit R. D., Zaia J. A., Valerio M. C., Levin M. J. 1982; Infection of human peripheral blood mononuclear cells by varicella-zoster virus. Intervirology 18:56–65
    [Google Scholar]
  2. Becker S. 1984; Influence of interferon on human monocyte to macrophage development. Cellular Immunology 84:145–153
    [Google Scholar]
  3. Bell D. M., Roberts N. J.JR Hall C. B. 1983; Different antiviral spectra of human macrophage interferon activities. Nature; London: 305319–321
    [Google Scholar]
  4. Boyum A. 1968; Isolation of mononuclear cells and granulocytes from human blood. Scandinavian Journal of Clinical and Laboratory Investigation 21:77–89
    [Google Scholar]
  5. Chonmaitree T., Roberts N. J.JR Douglas R. G.JR Hall C. B., Simons R. L. 1981; Interferon production by human mononuclear leukocytes: differences between respiratory syncytial virus and influenza viruses. Infection and Immunity 32:300–303
    [Google Scholar]
  6. Daniels C. A., Kleinerman E. S., Snyderman R. 1978; Abortive and productive infections of human mononuclear phagocytes by type I herpes simplex virus. American Journal of Pathology 91:119–129
    [Google Scholar]
  7. Domurat F., Roberts N. J.JR Walsh E. E., Dagan R. 1985; Respiratory syncytial virus infection of human mononuclear leukocytes in vitro and in vivo. Journal of Infectious Diseases 152:895–902
    [Google Scholar]
  8. Fernie B. F., Gerin J. L. 1980; The stabilization and purification of respiratory virus using MgSO4. Virology 106:141–144
    [Google Scholar]
  9. Fischer D., Van Der Weyden M. B., Snyderman R., Kelley W. N. 1976; A role for adenosine deaminase in human monocyte maturation. Journal of Clinical Investigation 58:399–407
    [Google Scholar]
  10. Glezen W. P., Loda F. A., Clyde W. A.JR Senior R. J., Sheaffer C. I., Conley W. G., Denny R. W. 1971; Epidemiologic patterns of acute lower respiratory disease of children in a pediatric group practice. Journal of Pediatrics 78:397–406
    [Google Scholar]
  11. Hall C. B., Douglas R. G.JR Simons R. L., Geiman J. M. 1978; Interferon production in children with respiratory syncytial, influenza and parainfluenza virus infections. Journal of Pediatrics 93:28–32
    [Google Scholar]
  12. Hall C. B., Douglas R. G.JR Simons R. L. 1981; Interferon production in adults with respiratory syncytial viral infection. Annals of Internal Medicine 94:53–55
    [Google Scholar]
  13. Havell E. A., Vilcek J. 1972; Production of high-titered inferferon in cultures of human diploid cells. Antimicrobial Agents and Chemotherapy 2:476–484
    [Google Scholar]
  14. Hendry R. M., McIntosh K. 1982; Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: development and description. Journal of Clinical Microbiology 16:324–328
    [Google Scholar]
  15. Linnavuori K., Hovi T. 1983; Restricted replication of herpes simplex virus in human monocytes cultures: role of interferon. Virology 130:1–9
    [Google Scholar]
  16. McIntosh K. 1978; Interferon in nasal secretions from infants with viral respiratory tract infections. Journal of Pediatrics 93:33a–36a
    [Google Scholar]
  17. McIntosh K., McQuillin J., Gardner P. S. 1979; Cell-free and cell-bound antibody in nasal secretions from infants with respiratory syncytial virus infection. Infection and Immunity 23:276–281
    [Google Scholar]
  18. Morahan P. S., Connor J. R., Leary K. R. 1985; Viruses and the versatile macrophage. British Medical Bulletin 41:15–21
    [Google Scholar]
  19. Mufson M. A., Krause H. E., Mocega H. E., Dawson F. w. 1970; Viruses, mycoplasma pneumoniae and bacteria associated with lower respiratory tract disease among infants. American Journal of Epidemiology 91:192–202
    [Google Scholar]
  20. Narayan O., Kennedy-Stoskopf S., Sheffer D., Griffin D. E., Clements J. E. 1983; Activation of caprine arthritis-encephalitis virus expression during maturation of monocytes to macrophages. Infection and Immunity 41:67–73
    [Google Scholar]
  21. Roberts N. J.JR 1982; Different effects of influenza virus, respiratory syncytial virus, and Sendai virus on human lymphocytes and macrophages. Infection and Immunity 35:1142–1146
    [Google Scholar]
  22. Sullivan J. L., Barry D. w., Lucas S. J., Albrecht P. 1975; Measles infection of human mononuclear cells. Journal of Experimental Medicine 142:773–784
    [Google Scholar]
  23. Toth T., Hesse R. A. 1983; Replication of five bovine respiratory viruses in cultured bovine alveolar macrophages. Archives of Virology 75:219–224
    [Google Scholar]
  24. Van Der Logt J. T. M., Van Loon A. M., Van Der Veen J. 1980; Replication of rubella virus in human mononuclear blood cells. Infection and Immunity 27:309–314
    [Google Scholar]
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