1887

Abstract

SUMMARY

Various mammalian cells propagated in serum-free and chemically defined media yielded high titres of Venezuelan equine encephalomyelitis virus. Some difference in maximum titres was noted, depending upon the medium employed. Of the two serum-free media tested, lactalbumin hydrolysate medium was more effective than the chemically defined medium in supporting virus growth. The addition of serum to serum-free cultures at the time of virus inoculation had a pronounced effect characterized by a delay followed by a burst of virus replication to very high titres. Thus, the degree of replication of Venezuelan equine encephalomyelitis virus appeared to be influenced by a variety of unknown nutritional factors.

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/content/journal/jgv/10.1099/0022-1317-10-3-231
1971-03-01
2024-04-19
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References

  1. Bryant J. C. 1966; Mammalian cells in chemically defined media in suspension cultures. Annals of the New York Academy of Sciences 139:595
    [Google Scholar]
  2. Graves J. H. 1963; Foot-and-mouth disease vaccine-the production of antigen in cultured cells in the absence of serum. American Journal of Veterinary Research 24:183
    [Google Scholar]
  3. Hardy R. M., Brown A. 1961; Growth of Venezuelan equine encephalomyelitis virus in L cells. II. Growth of submerged cultures. Journal of Bacteriology 82:449
    [Google Scholar]
  4. Hearn H. J. Jun. 1960; A variant of Venezuelan equine encephalomyelitis virus attenuated for mice and monkeys. Journal of Immunology 84:626
    [Google Scholar]
  5. Hearn H. J. Jun., Tribble H. R. Jun. 1965; Biological factors associated with the rapid attenuation of Venezuelan equine encephalomyelitis virus in chronically infected suspended L cells. Bacteriological Proceedings104.
    [Google Scholar]
  6. Higuchi K. 1963; Studies on the nutrition and metabolism of animal cells in serum-free media. I. Serum-free monolayer cultures. Journal of Infectious Diseases 112:213
    [Google Scholar]
  7. Mclimans W. F., Giardinello R. E., Davis E. V., Kucera C. J., Rake G. W. 1957; Submerged culture of mammalian cells: the five liter fermentor. Journal of Bacteriology 74:768
    [Google Scholar]
  8. Nagle S. C. Jun., Tribble H. R. Jun., Anderson R. E., Gary N. D. 1963; A chemically defined medium for growth of animal cells in suspension. Proceedings of the Society for Experimental Biology and Medicine 112:340
    [Google Scholar]
  9. Reed L. J., Muench H. 1938; A simple method for estimating fifty per cent end points. American Journal of Hygiene 27:493
    [Google Scholar]
  10. Taylor-Robinson D., Zwartouw H. T., Westwood J. C. N. 1961; The oxygen requirement for poliovirus replication in concentrated suspensions of ERK cells. British Journal of Experimental Pathology 42:317
    [Google Scholar]
  11. Tribble H. R. Jun., Hearn H. J., Soper W. T. 1963; Venezuelan equine encephalomyelitis virus particle selection in fluid suspension cultures. Bacteriological Proceedings154.
    [Google Scholar]
  12. Tribble H. R. Jun., Higuchi K. 1963; Studies on the nutrition and metabolism of animal cells in serum-free media. II. Cultivation of cells in suspension. Journal of Infectious Diseases 112:221
    [Google Scholar]
  13. Walker J. S., Carter R. C., Klein F., Snowden S. E., Lincoln R. E. 1969; Evaluation of factors related to growth of Rift Valley fever virus in suspended cell cultures. Applied Microbiology 17:658
    [Google Scholar]
  14. Waymouth C. 1959; Rapid proliferation of sublines of NCTC clone 929 (strain l) mouse cells in a simple chemically defined medium (MB752/1). Journal of the National Cancer Institute 22:1003
    [Google Scholar]
  15. Westwood J. C. N., Appleyard G., Taylor-Robinson D., Zwartouw H. T. 1960; The production of high titre poliovirus in concentrated suspensions of tissue culture cells. British Journal of Experimental Pathology 41:105
    [Google Scholar]
  16. Zwartouw H. T., Algar D. J. 1968; Growth of high-titre Semliki Forest virus in concentrated suspensions of chick embryo cells. Journal of General Virology 2:243
    [Google Scholar]
  17. Zwartouw H. T., Taylor-Robinson D., Westwood J. C. H. 1960; Growth of high-titre poliovirus in nutrient-free saline suspensions of ERK cells. Virology 10:393
    [Google Scholar]
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