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Volume 70, Issue 5

sp. nov., a novel bacterium isolated from the estuarine sediment of the Pearl River in China, and transfer of and to the genus as comb. nov. and comb. nov. Free

Abstract

A Gram-negative, aerobic, rod-shaped, non-motile by gliding bacterium was isolated from the estuarine sediment of the Pearl River in PR China and designated as strain q18. Colonies were circular, smooth and yellow on marine agar after 48 h cultivation. Salinity, temperature and pH for optimal growth were 5 % (NaCl), 30 °C and 7, respectively. The 16S rRNA gene sequence of the strain q18 showed the highest similarity of 97.3 % to the type strain of CC-CZW007. 16S rRNA gene-based phylogenetic analysis indicated that strain q18 grouped into the genus in the family of the phylum , and was distinct from all known species in the genus. Menaquinone (MK-6) was the main respiratory quinone detected in strain q18. The major fatty acids were iso-C and iso-C 3-OH. The polar lipids of strain q18 mainly comprised phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids and one unidentified polar lipid. The G+C content of the genome was ~42.8 mol%. The draft genome size of strain q18 was 3.3 Mbp. The average nucleotide identity values were around 79.0 % between strain q18 and reference strains. Based on the polyphasic analysis, strain q18 was confirmed to represent a novel species of the genus , for which the name sp. nov., is proposed. The type strain is q18 (=CICC 24821=KCTC 72764 ). Further, based on the results of phylogenetic, chemotaxonomic and phenotypic analyses, two species previously classified into the genus , Kim . 2018 and Thevarajoo . 2016, are transferred to the genus as comb. nov. and comb. nov. respectively.

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  • Accepted:
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  • Published Online:
Keyword(s): average nucleotide identity , genome , genotypic , phenotypic and phylogeny
Funding
This study was supported by the:
  • Guangdong Science and Technology Department (Award 2017KTSCX072)
    • Principle Award Recipient: Hui Wang
  • Department of Education of Guangdong Province (Award Pearl River Scholar for Young Investigator)
    • Principle Award Recipient: Hui Wang
  • Guangdong Science and Technology Department (Award SAIL Foundation for Distinguished Scholars)
    • Principle Award Recipient: Hui Wang
  • National Natural Science Foundation of China (Award 41676116)
    • Principle Award Recipient: Hui Wang
© 2020 The Authors
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2020-04-06
2024-04-27
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Aequorivita lutea sp. nov., a novel bacterium isolated from the estuarine sediment of the Pearl River in China, and transfer of Vitellibacter todarodis and Vitellibacter aquimaris to the genus Aequorivita as Aequorivita todarodis comb. nov. and Aequorivita aquimaris comb. nov.
Int J Syst Evol Microbiol 70, 3117 (2020); https://doi.org/10.1099/ijsem.0.004139
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