1887

Abstract

Summary

Axenic and monoxenic C3H mice were used to develop an animal model for enteroinvasiveness and translocation of . After oral administration of 10-10 viable cells of on day 0 (D0), bacterial colonisation was followed quantitatively during 23 days by counting free luminal bacteria and tissue-associated bacteria in the duodenum, ileum and colon. The kinetics of bacterial colonisation were the same in axenic and monoxenic mice; bacteria were more numerous in distal than in proximal intestinal segments.

Electronmicroscope studies of axenic infected mice showed free in the intestinal lumen on D2 and D7, and adhering to microvilli or included in enterocyte vacuoles in the colon on D2 without inflammatory reaction; was isolated from mesenteric lymph nodes until D23, but from blood, spleen, liver and bile until D1 only. In monoxenic infected mice, was found from D1 to D4 in mesenteric lymph nodes and Peyer's patches, whereas the associated bacterium () was never cultured from any organs.

On the basis of our observations in this gnotobiotic model, appears to be an enteroinvasive bacterium with a particular affinity for lymphoid organs.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-20-2-215
1985-10-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/20/2/medmicro-20-2-215.html?itemId=/content/journal/jmm/10.1099/00222615-20-2-215&mimeType=html&fmt=ahah

References

  1. Blaser M. J., Barth-Reller L. B. 1981; Campylobacter enteritis. New England Journal of Medicine 305:1444–1452
    [Google Scholar]
  2. Blaser M. J., Duncan D. J., Warren G. H., Wang W. L. 1983; Experimental Campylobacter jejuni infection of adult mice. Infection and Immunity 39:908–916
    [Google Scholar]
  3. Butzler J. P. ed 1984 Campylobacter infection in man and animals. C.R.C. Press; Boca Raton. FA:
    [Google Scholar]
  4. Butzler J., P Dekeyser P., Detrain M., De Haen F. 1973; Related vibrio in stools. Journal of Pediatrics 82:493–495
    [Google Scholar]
  5. Butzler J. P., Dereume J. P., Barbier P., Smekins L., De Keyser J. 1977; L’origine digestive des septicemies a Campylobacter. Nouvelle Presse Médicale 6:1033–1035
    [Google Scholar]
  6. Butzler J. P., Skirrow M. B. 1979; Campylobacter enteritis. Clinics in Gastroenterology 8:737–765
    [Google Scholar]
  7. Caldwell M. B., Walker R. I., Stewart S. D., Rogers J. E. 1983; Simple adult rabbit model for Campylobacter jejuni enteritis. Infection and Immunity 42:1176–1182
    [Google Scholar]
  8. Coates M. E. ed 1968; The germ-free animal in research. Academic Press; London:
    [Google Scholar]
  9. Fernandez H., Neto U. F., Fernandes F., de Almeida Pedra M., Trabulsi L. R. 1983; Culture supernatants of Campylobacter jejuni induce a secretory response in jejunal segments of adult rats. Infection and Immunity 40:429–431
    [Google Scholar]
  10. Field L. H., Underwood J. L., Pope L. M., Berry L. J. 1981; Intestinal colonisation of neonatal animals by Campylobacter fetus subsp. jejuni. Infection and Immunity 33:884–892
    [Google Scholar]
  11. Guerrant R. L., Lahita R. G., Winn W. C., Roberts R. B. 1978; Campylobacteriosis in man: pathogenic mechanisms and review of 91 blood stream infections. American Journal of Medicine 65:584–590
    [Google Scholar]
  12. Hanslo D., Fryer T., Le Roux E. 1983; Campylobacter bacteraemia in children. In Pearson AD., Skirrow MB. eds Abstracts of the 2nd International workshop on Campylobacter infections. Public Health Laboratory Service; London:
    [Google Scholar]
  13. King E. O. 1957; Human infections with Vibrio fetus, and a closely related vibrio. Journal of Infectious Diseases 101:119–128
    [Google Scholar]
  14. King E. O. 1962; The laboratory recognition of Vibrio fetus, and a closely related Vibrio isolated from cases of human vibriosis. Annals of the New York Academy of Sciences 98:700–711
    [Google Scholar]
  15. Madge D. S. 1980; Campylobacter enteritis in young mice. Digestion 20:389–394
    [Google Scholar]
  16. Manninen K. I., Prescott J. K., Dohoo I. R. 1982; Pathogenicity of Campylobacter jejuni isolates from animals and humans. Infection and Immunity 38:46–52
    [Google Scholar]
  17. Newell D. G. 1984; Experimental studies of Campylobacter enteritis. In Butzler J. P. ed Campylobacter infection in man and animals. C.R.C. Press; Boca Raton, FA: pp 113–132
    [Google Scholar]
  18. Newell D. G., Pearson A. D. 1982; Pathogenicity of Campylobacter jejuni—an in vitro model of adhesion and invasion?. In Newell D. G. ed Campylobacter, epidemiology, pathogenesis and biochemistry. MTP Press Ltd; Lancaster: pp 196–199
    [Google Scholar]
  19. Pearson A. D., Knott J. R., Suckling W. G., Tuck A. C. 1983; A serodiagnostic study of Campylobacter infection in 450 children with suspected appendicitis. In Pearson A. D., Skirrow M. B. eds Abstracts of the 2nd International workshop on Campylobacter infections. Public Health Laboratory Service; London:
    [Google Scholar]
  20. Pearson A. D., Skirrow M. B. eds Abstracts of the 2nd International workshop on Campylobacter infections. Public Health Laboratory Service; London:
    [Google Scholar]
  21. Prescott J. F., Barker I. K., Manninen K. I., Miniatsi OP. 1981; Campylobacter jejuni colitis in gnotobiotic dogs. Canadian Journal of Comparative Medicine 45:377–383
    [Google Scholar]
  22. Prescott J. F., Manninen K. I., Barker IK. 1982; Experimental pathogenesis of Campylobacter jejuni enteritis. Studies in gnotobiotic dogs, pigs and chickens. In Newell D. G. ed Campylobacter: epidemiology, pathogenesis and biochemistry. MTP Press Ltd; Lancaster: pp 170–171
    [Google Scholar]
  23. Price A. B., Jewkes J., Sanderson P. J. 1979; Acute diarrhoea: Campylobacter colitis and the role of rectal biopsy. Journal of Clinical Pathology 32:990–997
    [Google Scholar]
  24. Ruiz-Palacios G. M., Escamilla E., Torres N. 1981; Experimental Campylobacter diarrhoea in chickens. Infection and Immunity 34:250–255
    [Google Scholar]
  25. Ruiz-Palacios G. M., Torres J., Torres N. I., Escamilla E., Ruiz-Palacios B. R., Tamayo J. 1983; Cholera-like enterotoxin produced by Campylobacter jejuni. Characterisation and clinical significance. Lancet 2:250–253
    [Google Scholar]
  26. Skirrow M. B. 1982; Campylobacter enteritis—the first five years. Journal of Hygiene 89:175–184
    [Google Scholar]
  27. Soerjadi A. S., Snoeyenbos G. H., Weinack O. M. 1982; Intestinal colonisation and competitive exclusion of Campylobacter fetus subsp. jejuni in young chicks. Avian Diseases 26:520–524
    [Google Scholar]
  28. Steffen E. K., Berg R. D. 1983; Relationship between cecal population levels of indigenous bacteria and translocation to the mesenteric lymph nodes. Infection and Immunity 39:1252–1259
    [Google Scholar]
  29. Veron M. 1981; L’épidémiologie de la campylobactériose digestive. Gazette Médicale de France 88:2137–2144
    [Google Scholar]
  30. Veron M., Chatelain R. 1973; Taxonomic study of the genus Campylobacter Sebald and Véron and designation of the neotype strain for the type species Campylobacter fetus (Smith and Taylor) Sebald and Véron. International Journal of Systematic Bacteriology 23:122–134
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-20-2-215
Loading
/content/journal/jmm/10.1099/00222615-20-2-215
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error