1887

Abstract

SUMMARY: Mutants of PACI were isolated which, unlike the wild-type, could utilize phenylacetamide as a nitrogen source for growth. One group of these mutants had lost the ability to grow on acetamide; the second group grew slowly on acetamide. Group I included 12 mutants isolated from the magno-constitutive butyramide-utilizing strain PAC351 and one mutant isolated from the valeramide-utilizing mutant PAC360. Group I Ph amidases hydrolysed phenylacetamide, butyramide and valeramide but not acetamide or formamide. Group II Ph mutants produced thermolabile amidases which attacked the same range of amide substrates but in addition had weak activity towards acetamide. Three group II mutants were derived from a constitutive butyramide-regulator mutant PAC128; one from PAC360 and one from PAC326, a mutant producing a defective amidase.

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/content/journal/micro/10.1099/00221287-73-1-161
1972-11-01
2024-04-18
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References

  1. Brammar W. J., Clarke P. H. 1964; Induction and repression of Pseudomonas aeruginosa amidase. Journal of General Microbiology 37:307–319
    [Google Scholar]
  2. Brammar W. J., Clarke P. H., Skinner A. J. 1967; Biochemical and genetic studies with regulator mutants of the Pseudomonas aeruginosa 8602 amidase system. Journal of General Microbiology 47:87–102
    [Google Scholar]
  3. Brammar W. J., McFarlane N. D., Clarke P. H. 1966; The uptake of aliphatic amides by Pseudomonas aeruginosa . Journal of General Microbiology 44:303–309
    [Google Scholar]
  4. Brown J. E. 1969 Regulator and structural gene mutants of Pseudomonas aeruginosa amidase. Ph.D. Thesis: University of London.;
    [Google Scholar]
  5. Brown J. E., Brown P. R., Clarke P. H. 1969; Butyramide-utilizing mutants of Pseudomonas aeruginosa 8602 which produce an amidase with altered substrate specificity. Journal of General Microbiology 57:273–295
    [Google Scholar]
  6. Brown J. E., Clarke P. H. 1970; Mutations in a regulator gene allowing Pseudomonas aeruginosa 8602 to grow on butyramide. Journal of General Microbiology 64:329–342
    [Google Scholar]
  7. Brown P. R., Clarke P. H. 1972; Amino acid substitution in an amidase produced by an acetanilideutilizing mutant of Pseudomonas aeruginosa . Journal of General Microbiology 70:287–298
    [Google Scholar]
  8. Clarke P. H. 1972; Biochemical and immunological comparison of aliphatic amidases produced by Pseudomonas species. Journal of General Microbiology 71:241–257
    [Google Scholar]
  9. Demerec M., Adelburg E. A., Clark A. J., Hartman P. E. 1966; A proposal for a uniform nomenclature in bacterial genetics. Genetics 54:61–76
    [Google Scholar]
  10. Hegeman G. D., Rosenberg S. L. 1970; The evolution of bacterial enzyme systems. Annual Review of Microbiology 24:429–462
    [Google Scholar]
  11. Holloway B. W. 1969; Genetics of Pseudomonas . Bacteriological Reviews 33:419–443
    [Google Scholar]
  12. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  13. Mortlock R. P., Fossitt D. D., Wood W. A. 1965; A basis for utilization of unnatural pentoses and pentitols by Aerobacter aerogenes . Proceedings National Academy of Sciences of the United States of America 54:572–579
    [Google Scholar]
  14. Stanier R. Y., Palleroni N. J., Doudoroff M. 1966; The aerobic pseudomonads; a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  15. Stephenson M. 1949 Bacterial Metabolism,, 3rd edn. p. 311 London: Longmans, Green & Co.;
    [Google Scholar]
  16. Wu T., Lin E. C., Tanaka S. 1968; Mutants of Aerobacter aerogenes capable of utilizing xylitol as a novel carbon source. Journal of Bacteriology 96:447–456
    [Google Scholar]
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