1887

Abstract

SUMMARY

The originally infected ear of mice latently infected in the cervical ganglia with herpes simplex virus (HSV) was treated with one of five stimuli: stripping with cellophane tape, irradiation with u.v. light, or the application of xylene, dimethyl sulphoxide (DMSO) or retinoic acid. Each of these stimuli induced the appearance of infectious virus in the ganglia 1 to 5 days later, most frequently after 1 to 3 days. Virus was also isolated from the treated ears, most frequently 3 to 5 days after stimulation. In a proportion of mice treated with cellophane tape stripping, xylene, retinoic acid or DMSO, clinical recurrent disease was observed, although in the case of DMSO this proportion was low. Some of the physiological changes induced in the skin by the five stimuli were studied. Treatment with DMSO, cellophane tape stripping or xylene induced almost immediate inflammation in the skin as judged by extravasation of Evans blue dye. Studies with inhibitors suggested that this was mediated by a neurogenic factor together with histamine or 5-hydroxytryptamine, or both of these. In addition, with the exception of mice treated with DMSO, the levels of prostaglandins of the E and F classes in the skin of the ear were elevated 1 to 3 days after treatment. These results are discussed with reference to the mechanisms by which recurrent herpetic disease develops.

Keyword(s): HSV , inflammation , reactivation and recurrence
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-7-1491
1983-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/7/JV0640071491.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-7-1491&mimeType=html&fmt=ahah

References

  1. Blyth W. A., Hill T. J., Field H. J., Harbour D. A. 1976; Reactivation of herpes simplex virus infection by ultraviolet light and possible involvement of prostaglandins. Journal of General Virology 33:547–550
    [Google Scholar]
  2. Blyth W. A., Harbour D. A., Hill T. B. 1980; Effect of immunosuppression on recurrent herpes simplex in mice. Infection and Immunity 29:902–907
    [Google Scholar]
  3. Cornette J. C., Kirton K. T., Barr K. L., Forbes A. D. 1972; Radioimmunoassay of prostaglandins. In The Prostaglandins. Clinical Applications in Human Reproduction pp 243–255 Edited by Southern E. M. New York: Futura;
    [Google Scholar]
  4. Di Rosa M., Giroud J. P., Willoubhy D. A. 1971; Studies of the mediators of the acute inflammatory response induced in rats in different sites by carrageenan and turpentine. Journal of Pathology 104:15–29
    [Google Scholar]
  5. Green A. F., Garland L. G., Hodson H. F. 1979; Antagonists of histamine, 5-hydroxytryptamine and SRS-A. Handbook of Experimental Pharmacology 50:415–466
    [Google Scholar]
  6. Harada M., Takeuchi M., Fukao T., Katagiri K. 1971; A simple method for the quantitative extraction of dye extravasated into the skin. Journal of Pharmacy and Pharmacology 23:218–219
    [Google Scholar]
  7. Harbour D. A., Blyth W. A., Hill T. J. 1978; Prostaglandins enhance spread of herpes simplex virus in cell cultures. Journal of General Virology 41:87–95
    [Google Scholar]
  8. Harbour D. A., Hill T. J., Blyth W. A. 1981; Acute and recurrent herpes simplex in several strains of mice. Journal of General Virology 55:31–40
    [Google Scholar]
  9. Hill T. J. 1981; Mechanisms involved in recurrent herpes simplex. In The Human Herpesviruses. An Interdisciplinary Perspective pp 241–244 Edited by Nahmias A. J., Dowdle W. R., Schinazi R. F. New York: Elsevier/North-Holland;
    [Google Scholar]
  10. Hill T. J. 1983; Herpes simplex virus latency. In The Herpesviruses Edited by Roizman B. (Comprehensive Virology series 2) New York: Plenum Press; (in press)
    [Google Scholar]
  11. Hill T. J., Blyth W. A. 1976; An alternative theory of herpes simplex recurrence and a possible role for prostaglandins. Lancet 1:397–399
    [Google Scholar]
  12. Hill T. J., Field H. J., Blyth W. A. 1975; Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. Journal of General Virology 28:341–353
    [Google Scholar]
  13. Hill T. J., Blyth W. A., Harbour D. A. 1978; Trauma to the skin causes recurrence of herpes simplex in the mouse. Journal of General Virology 39:21–28
    [Google Scholar]
  14. Hill T. J., Blyth W. A., Harbour D. A. 1982; Recurrent herpes simplex in mice: topical treatment with acyclovir cream. Antiviral Research 2:135–146
    [Google Scholar]
  15. Hillier K., Kasonde J. M. 1976; Prostaglandin E and F concentrations in human endometrium after insertion of intrauterine contraceptive device. Lancet i:15–16
    [Google Scholar]
  16. Jancso N. 1960; Role of the nerve terminals in the mechanism of inflammatory reactions. Bulletin of the Millard Fillmore Hospital 7:53–77
    [Google Scholar]
  17. Price R. W., Schmitz J. 1978; Reactivation of latent herpes simplex virus infection of the autonomic nervous system by postganglionic neurectomy. Infection and Immunity 19:523–532
    [Google Scholar]
  18. Salmon J. A., Karim S. M. M. 1976; Methods for analysis of prostaglandins. In Prostaglandins: Chemical and Biochemical Aspects pp 25–85 Edited by Karim S. M. M. Lancaster: MTP Press;
    [Google Scholar]
  19. Trofatter K. F., Daniels C. A. 1979; Interaction of human cells with prostaglandins and cyclic AMP modulators. Journal of Immunology 122:1363–1370
    [Google Scholar]
  20. Walker S. B., Chahl L. A. 1980; The mechanism of enhancement of the neurogenic oedema response by baclofen. European Journal of Pharmacology 62:27–34
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-7-1491
Loading
/content/journal/jgv/10.1099/0022-1317-64-7-1491
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error