1887

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Volume 25, Issue 3

Isolation and Preliminary Characterization of Temperature-sensitive Mutants of Sindbis Virus Strain AR339 Free

Abstract

Summary

One hundred and four temperature-sensitive mutants of Sindbis virus strain AR339 have been isolated using five different mutagens. Forty-seven mutants showed no detectable RNA synthesis at the restrictive temperature (39 °C), whereas 57 showed levels ranging from 1% to over 100% of the wild-type level. The mutants did not show complementation. The ratio of the 42S:26S RNA species in cells infected with 30 mutants showing > 10% of the wild-type level of RNA synthesis at 39 °C was measured. Of these 30 mutants, none were found which were wholly defective in production of either RNA species, although the ratio was significantly different from the wild-type for some mutants. Two mutants showed production at 39 °C of polydisperse RNA of both double-stranded and single-stranded types.

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© Journal of General Virology 1974
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1974-12-01
2024-04-28
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Isolation and Preliminary Characterization of Temperature-sensitive Mutants of Sindbis Virus Strain AR339
J. Gen. Virol. 25, 371 (1974); https://doi.org/10.1099/0022-1317-25-3-371
/content/journal/jgv/10.1099/0022-1317-25-3-371
/content/journal/jgv/10.1099/0022-1317-25-3-371
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