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Toshiaki Nakano, Atsushi Katafuchi, Hiroaki Terato, Toshinori Suzuki, Bennett Van Houten, Hiroshi Ide, Activity of nucleotide excision repair enzymes for oxanine cross-link lesions, Nucleic Acids Symposium Series, Volume 49, Issue 1, September 2005, Pages 293–294, https://doi.org/10.1093/nass/49.1.293
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Abstract
Nitric oxide and nitrous acid induce deamination of DNA bases, resulting in uracil, hypoxanthine, xanthine, and oxanine (Oxa) as major damage. Oxa reacts further with polyamines and DNA binding proteins, generating bulky cross-link adducts. Recently we have shown Oxa and cross-link adducts are potentially genotoxic lesions. In the present study, we have assessed the role of base excision repair (BER) and nucleotide excision repair (NER) systems in the repair of Oxa and Oxa-spermine (Oxa-Sp) cross-link adducts. Oxa was very poorly removed from DNA by both BER glycosylases and NER enzymes, whereas Oxa-Sp was efficiently excised by E. coli and human NER enzymes.
Author notes
1Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University, Higashi-Hiroshima 739-8526, Japan, 2Department of Biological Pharmacy, School of Pharmacy, Shujitu University, 1-6-1 Nishigawara, Okayama 703-8516, Japan, 3Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institute of Health, Research Triangle Park, NC 27709, USA