Facemasks and Public Health: analysis of bacterial contamination in FFP2 masks

Abstract Background Facemasks (FM), due to the Covid-19 pandemic, are extensively used and often worn beyond the recommended time. This has led to questions about the negative impact persistent contamination on FMs might have on public health. The study aims to assess the level of contamination reached in a small cohort of subjects after the recommended use (8 h) of FM. Methods This descriptive study was carried out between January and April 2022 on 17 people: 9 women and 8 men aged between 25-45 years. These two groups were divided into two micro-groups: women were selected according to their skincare habits (no skincare and skincare with cosmetics). In contrast, men were selected according to the length of their beards (thick or short beard). The FM was worn for 8 h in a controlled office setting, to avoid possible uncontrolled variables. Then, the FM was cut, placed in a tube with a recovery medium and centrifuged. The supernatant was removed and the pellet resuspended. Aliquots were plated on Petri plates and incubated for 48 h at 36 °C to count the Colony Forming Units (CFU). The statistical analysis was conducted using Stata software, performing the Wilcoxon matched-pairs and setting a significance level of p < 0.05. Results Women had higher FM contamination than men (= 4960 vs 3130 CFU/ml). Also, we found more colonies (= 18890 vs 3420 CFU/ml) in the FMs of women without skincare (p = 0.06), while among men, more colonies were reported for those with a thicker beard than for those with a shorter one (= 3300 vs 2960 CFU/ml). Conclusions Extensive FM use increases bacterial contamination exponentially. This could lead to changes in the facial microbiome, inducing skin conditions (such as allergic dermatitis and acne). Facial skin conditions are important public health issues for people wearing FMs daily. In addition, responsible handling of this equipment is essential to avoid the spread of SARS-CoV-2 through contact with these items, which can persist for many days. Key messages • Gender and physical characteristics may influence the level of contamination present on FFP2 face masks. • There is a need to increase community awareness on the proper handling of facemasks, prevent health problems for users, and limit the spread of infection to those around them.

The COP26 conference in Glasgow, November 2021 presented a unique public health challenge in the midst of the COVID-19 pandemic. With 38,457 delegates attending, this international event posed a risk for SARS-CoV-2 infection. We used Targeted Hygiene theory to carry out a large event risk assessment process that focused on how spaces were used, referred to evidence from scientific literature, and identified key moments for surface and hand hygiene interventions. We relied on behavioural science evidence to optimize hand hygiene compliance at the event. To do so, we secured the opportunity to use hand sanitizer by determining the most suitable locations for over 500 hand sanitizing stations. To further motivate uptake of personal hygiene, kits were provided for each delegate with hygiene messaging to improve knowledge of the importance of hand hygiene in breaking the chain of infection. A COP26-branded face covering, personal hand gel and wipes were provided in the pack. Training and cleaning protocols centred on Targeted Hygiene were developed for cleaning teams to implement. Compliance monitoring through observation and daily real-time reporting of over 250 adenosine triphosphate (ATP) checks on just-cleaned surfaces provided reassurance for public health agencies that our Targeted Hygiene approach was effective. The messaging and cleaning interventions were carried out for the duration of the COP26 conference. The number of individuals officially affiliated with COP26 that tested positive was 2 in 1,000 as compared to 11-12 in 1,000 individuals in Scotland during the same period (6-13 November 2021 as reported by Public Health Scotland). Whilst no single control can be attributed to this achievement, effective hand and surface hygiene interventions contributed by helping to break the chain of infection. This risk-based approach to Targeted Hygiene serves as a blueprint for effective, sustainable and measurable nonpharmaceutical interventions at large scale events.

Key messages:
To mitigate risk of infection at COP26, key moments for surface and hand hygiene were identified and emphasized in cleaning protocols and education. Using a risk-based approach to Targeted Hygiene serves as a blueprint for effective, sustainable and measurable nonpharmaceutical interventions at large scale events such as COP26.
Abstract citation ID: ckac131.346 The effect of social desirability bias on the measurement of subjective health literacy of children

Background:
When measuring children's health literacy (HL) with selfreport questionnaires, there is doubt whether high scores reflect good HL, or if they are caused by social desirability bias, meaning that children present themselves favorably. This study explores how the tendency to answer in a socially desirable way impacts HL scores.

Methods:
A cross-sectional study was conducted among fourth-graders in North-Rhine Westphalia, Germany. The study was designed as a representative survey starting in 12/2020, which could not be realized due to pandemic-related constraints. Data collected between 07/21 and 11/21 is included here. Subjective HL was assessed with the HLS-Child-Q15 and a validated 10-item scale (AFS) was used for social desirability bias.

Methods:
This descriptive study was carried out between January and April 2022 on 17 people: 9 women and 8 men aged between 25-45 years. These two groups were divided into two microgroups: women were selected according to their skincare habits (no skincare and skincare with cosmetics). In contrast, men were selected according to the length of their beards (thick or short beard). The FM was worn for 8 h in a controlled office setting, to avoid possible uncontrolled variables. Then, the FM was cut, placed in a tube with a recovery medium and centrifuged. The supernatant was removed and the pellet resuspended. Aliquots were plated on Petri plates and incubated for 48 h at 36 C to count the Colony Forming Units (CFU). The statistical analysis was conducted using Stata software, performing the Wilcoxon matched-pairs and setting a significance level of p < 0.05.
15th European Public Health Conference 2022 Results: Women had higher FM contamination than men (x = 4960 vs 3130 CFU/ml). Also, we found more colonies (x = 18890 vs 3420 CFU/ml) in the FMs of women without skincare (p = 0.06), while among men, more colonies were reported for those with a thicker beard than for those with a shorter one (x = 3300 vs 2960 CFU/ml).

Conclusions:
Extensive FM use increases bacterial contamination exponentially. This could lead to changes in the facial microbiome, inducing skin conditions (such as allergic dermatitis and acne). Facial skin conditions are important public health issues for people wearing FMs daily. In addition, responsible handling of this equipment is essential to avoid the spread of SARS-CoV-2 through contact with these items, which can persist for many days.
Gender appears to be a strong predictor of online health information-seeking behaviour (OHISB). Gender differences in OHISB have been studied in different countries with different results, but no studies investigated gender-specific behaviour among University students during the COVID-19 pandemic, which has brought with it a consequential infodemic. We sought to investigate any gender differences in OHISB in the period between the first and the second wave of the COVID-19 pandemic. A questionnaire promoted by the COVID-HL network was administered to 2996 students of all the courses at the University of Florence. It included existing validated scales adapted to the COVID-19 pandemic and newly developed scales. Gender differences were tested using the 2 test or the Mann-Whitney U test, where appropriate. Male students reported a higher score in DHL than females (p < 0.001). However, female students reported using more often different sources for online information seeking (p < 0.05, except for YouTube), searching more corona-related topics (p < 0.05, except for economic and social consequences) and considering ''very important'' each item in the ''Importance of internet information search'' section (p < 0.05). Furthermore, female students are more likely to be ''often dissatisfied'' or ''partly satisfied'' with information about COVID-19 (p < 0.001) and to search more often for information for themselves and other people. Our study confirmed that gender could affect the way students search for health information on the Internet. Since students, in particular females, have been affected by stress and anxiety during the pandemic, these findings could help institutions to