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GEBRAN N. KARAM, Biomechanical Model of the Xylem Vessels in Vascular Plants, Annals of Botany, Volume 95, Issue 7, June 2005, Pages 1179–1186, https://doi.org/10.1093/aob/mci130
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Abstract
• Background and Aims The xylem, or water transport system, in vascular plants adopts different morphologies that appear sequentially during growth phases. This paper proposes an explanation of these morphologies based on engineering design principles.
• Methods Using microscopic observations of the different growth stages, an engineering analysis of the xylem vessels as a closed cylinder under internal pressure is carried out adopting pressure vessel design concepts.
• Key Results The analysis suggests that the xylem vessel structural morphology follows the ‘constant strength’ design principle, i.e. all of the material within the wall of the xylem is loaded equally to its maximum allowable stress capacity, and the amount of material used is therefore systematically minimized. The analysis shows that the different structural designs of the xylem vessel walls (annular, helical, reticulate and pitted) all quantitatively follow the constant strength design principle.
• Conclusions The results are discussed with respect to growth and differentiation. It is concluded that the morphology of the xylem vessel through the different phases of growth seems to follow optimal engineering design principles.
INTRODUCTION
The xylem is the principal water conducting tissue in a vascular plant. It is divided between primary and secondary xylem. Primary xylem consists of an early part, the protoxylem, which differentiates and matures among actively elongating plant organs, and a later part, the metaxylem, which initiates during the growth of the primary body of the plant but matures after elongation has ceased. Secondary xylem forms during secondary growth stages and initiates after all elongation has been completed. Cell walls in the tracheary elements of the xylem have a variety of secondary wall thickenings. Different types appear in an ontogenetic sequence with annular thickening occurring first, followed in order by helical, scalariform, reticulate and pitted thickening. Occurrence of the secondary wall types depends on the growth and maturity of the tracheary element and cannot be assigned distinctly to any one type of xylem. Two types of tracheary elements can be distinguished: tracheids and vessel elements. Tracheids appear mainly in woody plants and are connected laterally through multiple pits. Vessels appear in both woody and non-woody plants and are built of numerous vessel members joined at their ends. They are typically found in vascular bundles inside different plant organs (Fig. 1).
In this study, the biomechanics of vessel elements are analysed from an engineering point of view. An engineering model is derived by assuming that the design of the wall thickening follows a constant strength design principle, which, by loading all parts to their maximum allowable stress capacity, minimizes the amount of material used. The quantitative and qualitative predictions of this engineering model are compared with the structural designs of plant xylem vessel cell walls as reported in the published literature and as observed on some herbaceous and non-woody vascular plants to provide a new functional explanation for the different observed types of secondary wall thickening: annular, helical, reticulate and pitted.
BIOMECHANICS OF THE VESSEL ELEMENTS
From an engineering point of view, the xylem is the water distribution network that transmits water from the root collection system to the main consumers, the leaves, in the upper parts of the plant. Transpiration of the leaf mesophyll cells causes a water potential difference between the leaf and the xylem, resulting in water transport. During the growing season the water is lifted up to the leaves by negative pressures, less than atmospheric, created by transpiration. Vessels, made of elongated hollow cells connected end to end (Zimmerman, 1983), are subjected to high internal negative pressures (Choat et al., 2003) that must be resisted by their walls to prevent cell collapse. Microscopic investigation shows that primary vessel walls are reinforced by the secondary wall, a lignified cell-wall thickening, deposited on the inside of the primary wall of the cell (Wooding and Northcote, 1964; Bierhorst and Zamora, 1965; Ray, 1972; Neushul, 1974) (Fig. 2). Secondary wall first appears as an annular thickening. Subsequently, the thickening becomes helical, followed by interconnected helices, coils or scalariform thickening. The final structure, encountered in the last ontogenetic stage, has reticulate or net-like thickening, or uniform thickening with staggered pits in pitted vessels (Fig. 2) (Bierhorst and Zamora, 1965; Jensen and Salisbury, 1972; Ray, 1972; Neushul, 1974; Esau, 1977; Fahn, 1990). The type of secondary wall thickening is determined by the dimension (diameter) of the vessel and the maturity and growth stage of the plant (ontogeny). The literature contains many descriptions and discussions of the secondary wall patterns without an exact analysis or explanatory model. The mechanical engineering design problem of the xylem vessel element is treated in the next section to provide a possible explanation for these observations.
In addition to mechanically resisting the water pressure, the xylem also has to satisfy other design constraints such as plant growth strains, hydraulic conductivity and connectivity between adjacent cells. These will not be considered in the derivation of the engineering model.
ENGINEERING MODEL
The vessel element is analysed as a closed-end cylinder, of uniform radius, R, and wall thickness, t, subjected to an internal pressure, p, which can be either negative or positive; engineers call such a structure a ‘pressure vessel’ (Fig. 3).
The primary cell wall is a layer made of randomly orientated cellulose microfibrils in a relatively visco-elastic matrix that allows extension and elongation (Bodig and Jane, 1982; Niklas, 1992). Some re-orientation of the microfibrils is thought to take place under elongation stresses during growth. There is evidence that microfibrils get deposited mostly along the transverse or hoop direction before some get realigned along the fibre axis (Niklas, 1992) due to elongation stresses. For the purposes of the analysis the primary wall is considered to be homogeneous and isotropic with a maximum allowable stress of σ* both axially and transversely.
The secondary wall layer of xylem tracheids is known to consist of highly orientated cellulose microfibrils with a structure stabilized by lignin (Mark, 1967; Bodig and Jane, 1982; Niklas, 1992; Reiterer et al., 1999). The secondary wall of tracheids contributes over 80 % of the cell wall thickness and provides mechanical support to the plant. The same structure of highly orientated cellulose microfibrils is usually assumed for the secondary wall layer of xylem vessels in woody and non-woody plants, albeit the structural support function to the plant is not required. The cellulose microfibrils in the secondary wall of vessels are expected to be more-or-less aligned along the direction of deposition (annular, helical, etc.) with varying amounts of lignin deposited along the microfibrils. These factors result in differing strengths between the primary and secondary layers. In order to allow for this possible difference, a strength ratio, r, is introduced where the maximum allowable stress of the secondary wall layer along the direction of its deposition would be rσ*. The secondary wall layer, which is deposited along circular, spiral or net-like patterns, acts as a unidirectional reinforcement and hence only its axial properties are relevant to the analysis. The anisotropy of the secondary wall does not affect the model. It is worthwhile noting that the strength ratio, r, could be derived from basic composite theory as r = Esw/Epw; where Esw is the modulus of elasticity of the secondary wall, and Epw is the modulus of elasticity of the primary wall, when both walls are subjected to the same strains. The use of this strength ratio would account in the most general way for the difference in properties between the primary and secondary walls. When r = 1, the secondary and primary walls are assumed to have the same maximum allowable stress.
Note that a positive pressure (cell pressure greater than atmospheric) leads to tensile stresses in the wall while a negative pressure (cell pressure less than atmospheric) leads to compressive stresses. If the maximum allowable stress in the primary wall material is σ*, then the available resisting hoop force per unit length will be σ*t. Depending on the relative magnitudes of the pressure, the allowable stress in the wall material and the geometry of the tube, three cases will be encountered, each of which is analysed below.
Case I. σ*t ≥ pR, which implies that the tube can readily resist the hoop and longitudinal stresses caused by the internal pressure.
Equation 2 can be readily solved as an equality for the minimum A/s and an appropriate reinforcement can be designed.
Note that eqns (3)–(6) are identical for α and −α, which means that the reinforcement is equally effective whether the winding is from left to right or right to left; for a helix this translates into positive or negative curl (by the right hand rule).
When reinforcing helices of opposite curls are provided then the added area of the reinforcement also resists shears and the available shear resistance increases with the reinforcement ratio. Inequality (8) can be rewritten to give the limiting wall thickness before a double helix with opposite curls is required.
The above analysis is based on the ‘constant strength’ design principle (Flugge, 1966): the optimum design is that which stresses all material to the maximum allowable stress. Xylem vessels formed during growth and elongation of the plant, the protoxylem, are subjected to high longitudinal tensile stresses (Paolillo and Rubin, 1991; Niklas, 1992) in addition to the negative internal pressure: they fail by rupture of the primary wall and collapse under excessive elongation and internal pressure (Figs 1 and 2). The primary xylem vessels that mature after elongation ceases, the metaxylem, are subject only to internal pressure: negative in the normal situation of leaf transpiration and upward ‘pulling’ of water, and positive during the rare occasions of suppressed transpiration or very high humidity (Zimmerman, 1983). Failure can occur either by cell wall yielding or buckling: cell wall yield refers to material failure while buckling refers to a geometric instability failure (for instance, a light plastic ruler bowing out of shape when compressed at its ends or a bent straw kinking on the compressive face). Yielding of the vessel cell wall can occur under the combination of tensile (positive pressure) or compressive (negative pressure) hoop and longitudinal stresses. Buckling can occur under the combined effect of the hoop and longitudinal compressive stresses due to positive pressure; this type of failure is similar to that of a closed-end cylindrical shell under external hydrostatic pressure.
Plant type . | Wall thickness, t (μm) . | Radius, R (μm) . | Length, L (mm) . | Modulus of elasticity, E (GPa) . | Yield strength, σy (MPa) . | Source of data . |
---|---|---|---|---|---|---|
Lily stem (Lilium) | 0·5–1·0 | 15–25 | 0·5–1·0 | –a | – | SEMb investigation |
Monocotyledons | – | – | 0·76–3·96 | – | – | Cheadle (1943) |
Plant stems and roots | – | – | – | 2·8–7·0 | 50–120 (tensile) 6–11 (shear) | Blahovec (1988) |
Iris leaf | – | – | – | 2·7–7·1 | – | Gibson et al. (1988) |
Hardwoods | – | 20–350 | 0·2–1·3 | – | – | Bodig and Jayne (1982) |
Wood | – | – | – | 35 | 120 | Gibson and Ashby (1988) |
Oak latewood (Quercus rubra) | 1–6 | 30–100 | 0·2–0·4 | – | – | Gibson and Ashby (1988), Siau (1984) |
Plant type . | Wall thickness, t (μm) . | Radius, R (μm) . | Length, L (mm) . | Modulus of elasticity, E (GPa) . | Yield strength, σy (MPa) . | Source of data . |
---|---|---|---|---|---|---|
Lily stem (Lilium) | 0·5–1·0 | 15–25 | 0·5–1·0 | –a | – | SEMb investigation |
Monocotyledons | – | – | 0·76–3·96 | – | – | Cheadle (1943) |
Plant stems and roots | – | – | – | 2·8–7·0 | 50–120 (tensile) 6–11 (shear) | Blahovec (1988) |
Iris leaf | – | – | – | 2·7–7·1 | – | Gibson et al. (1988) |
Hardwoods | – | 20–350 | 0·2–1·3 | – | – | Bodig and Jayne (1982) |
Wood | – | – | – | 35 | 120 | Gibson and Ashby (1988) |
Oak latewood (Quercus rubra) | 1–6 | 30–100 | 0·2–0·4 | – | – | Gibson and Ashby (1988), Siau (1984) |
–, information not available;
scanning electron microsope.
Plant type . | Wall thickness, t (μm) . | Radius, R (μm) . | Length, L (mm) . | Modulus of elasticity, E (GPa) . | Yield strength, σy (MPa) . | Source of data . |
---|---|---|---|---|---|---|
Lily stem (Lilium) | 0·5–1·0 | 15–25 | 0·5–1·0 | –a | – | SEMb investigation |
Monocotyledons | – | – | 0·76–3·96 | – | – | Cheadle (1943) |
Plant stems and roots | – | – | – | 2·8–7·0 | 50–120 (tensile) 6–11 (shear) | Blahovec (1988) |
Iris leaf | – | – | – | 2·7–7·1 | – | Gibson et al. (1988) |
Hardwoods | – | 20–350 | 0·2–1·3 | – | – | Bodig and Jayne (1982) |
Wood | – | – | – | 35 | 120 | Gibson and Ashby (1988) |
Oak latewood (Quercus rubra) | 1–6 | 30–100 | 0·2–0·4 | – | – | Gibson and Ashby (1988), Siau (1984) |
Plant type . | Wall thickness, t (μm) . | Radius, R (μm) . | Length, L (mm) . | Modulus of elasticity, E (GPa) . | Yield strength, σy (MPa) . | Source of data . |
---|---|---|---|---|---|---|
Lily stem (Lilium) | 0·5–1·0 | 15–25 | 0·5–1·0 | –a | – | SEMb investigation |
Monocotyledons | – | – | 0·76–3·96 | – | – | Cheadle (1943) |
Plant stems and roots | – | – | – | 2·8–7·0 | 50–120 (tensile) 6–11 (shear) | Blahovec (1988) |
Iris leaf | – | – | – | 2·7–7·1 | – | Gibson et al. (1988) |
Hardwoods | – | 20–350 | 0·2–1·3 | – | – | Bodig and Jayne (1982) |
Wood | – | – | – | 35 | 120 | Gibson and Ashby (1988) |
Oak latewood (Quercus rubra) | 1–6 | 30–100 | 0·2–0·4 | – | – | Gibson and Ashby (1988), Siau (1984) |
–, information not available;
scanning electron microsope.
Source of data . | Wall thickness, t (μm) . | Thickening spacing, s (μm) . | Thickening area, A (μm2) . | Estimated angle, α (degrees) . | Radius of vessel, R (μm) . |
---|---|---|---|---|---|
Esau 1977, fig. 8.10a | 0·38 | 1·82 | 0·87 | 10–20 | 3·60 |
Esau 1977, fig. 8.12a | 0·38 | 3·50 | 1·56 | 10–17 | 4·25 |
Jensen and Salisbury, 1972, p.409 | 0·5 | 6·0 | 4·22 | 12–17 | 5·0 |
Roberts, 1976, fig. 27 | 0·5 | 1·81 | 0·91 | 0–3 | 6·88 |
SEMa investigation (Lilium) | 0·54 | 5·36 | 3·06 | 9 | 15·71 |
SEMa investigation (Lilium) | 0·91 | 6·97 | 3·24 | 0 | 7·88 |
Source of data . | Wall thickness, t (μm) . | Thickening spacing, s (μm) . | Thickening area, A (μm2) . | Estimated angle, α (degrees) . | Radius of vessel, R (μm) . |
---|---|---|---|---|---|
Esau 1977, fig. 8.10a | 0·38 | 1·82 | 0·87 | 10–20 | 3·60 |
Esau 1977, fig. 8.12a | 0·38 | 3·50 | 1·56 | 10–17 | 4·25 |
Jensen and Salisbury, 1972, p.409 | 0·5 | 6·0 | 4·22 | 12–17 | 5·0 |
Roberts, 1976, fig. 27 | 0·5 | 1·81 | 0·91 | 0–3 | 6·88 |
SEMa investigation (Lilium) | 0·54 | 5·36 | 3·06 | 9 | 15·71 |
SEMa investigation (Lilium) | 0·91 | 6·97 | 3·24 | 0 | 7·88 |
Scanning electron microscope.
Source of data . | Wall thickness, t (μm) . | Thickening spacing, s (μm) . | Thickening area, A (μm2) . | Estimated angle, α (degrees) . | Radius of vessel, R (μm) . |
---|---|---|---|---|---|
Esau 1977, fig. 8.10a | 0·38 | 1·82 | 0·87 | 10–20 | 3·60 |
Esau 1977, fig. 8.12a | 0·38 | 3·50 | 1·56 | 10–17 | 4·25 |
Jensen and Salisbury, 1972, p.409 | 0·5 | 6·0 | 4·22 | 12–17 | 5·0 |
Roberts, 1976, fig. 27 | 0·5 | 1·81 | 0·91 | 0–3 | 6·88 |
SEMa investigation (Lilium) | 0·54 | 5·36 | 3·06 | 9 | 15·71 |
SEMa investigation (Lilium) | 0·91 | 6·97 | 3·24 | 0 | 7·88 |
Source of data . | Wall thickness, t (μm) . | Thickening spacing, s (μm) . | Thickening area, A (μm2) . | Estimated angle, α (degrees) . | Radius of vessel, R (μm) . |
---|---|---|---|---|---|
Esau 1977, fig. 8.10a | 0·38 | 1·82 | 0·87 | 10–20 | 3·60 |
Esau 1977, fig. 8.12a | 0·38 | 3·50 | 1·56 | 10–17 | 4·25 |
Jensen and Salisbury, 1972, p.409 | 0·5 | 6·0 | 4·22 | 12–17 | 5·0 |
Roberts, 1976, fig. 27 | 0·5 | 1·81 | 0·91 | 0–3 | 6·88 |
SEMa investigation (Lilium) | 0·54 | 5·36 | 3·06 | 9 | 15·71 |
SEMa investigation (Lilium) | 0·91 | 6·97 | 3·24 | 0 | 7·88 |
Scanning electron microscope.
Using these typical numbers in eqn (13) the ration py/pcr varies from 0·6 to 4·0; we expect yielding to dominate in some cases while buckling would dominate in others similarly to the behaviour of tubular structures encountered in nature (Karam and Gibson, 1994). The clarification of this issue requires detailed experimental measurements on specific samples, and falls beyond the scope of this work. In the rest of this paper it will be assumed that the main purpose of the secondary wall reinforcement thickening is to prevent yielding of the walls.
DISCUSSION
Geometry of secondary wall thickening
The botanical literature gives a wide range of descriptions of secondary wall thickening patterns in xylem vessels (Goodwin, 1942; Wooding and Northcote, 1964; Jensen and Salisbury, 1972; Ray, 1972; Neushul, 1974; Esau, 1977; Fahn, 1990). Here those descriptions are compared with the geometries suggested by the engineering model developed in the previous section. It is noted that the optimum design from the structural perspective must also ensure the cell opening is large enough to maintain hydraulic efficiency, allowing the flow of fluid between cells. For case II where only hoop reinforcement is needed, the optimal geometry of the secondary wall is given by equally spaced rings or annuli as determined from eqn (2). For case III when only a little reinforcement is needed, i.e. A/s as determined from eqn (5) is small, inclined rings or a helical thickening will do. If more reinforcement is required, a second helix with the same curl can be added with the condition that s, the spacing, has to be larger than the thickness of the helix, so that helices do not merge. If the shear resistance of the xylem wall is exceeded, as well as its hoop and longitudinal resistance, then the addition of a second helix of opposite curl will be required. As the pressure or the radius increase, eqn (5) calls for larger reinforcement ratios, A/s. Noting that the addition of similar-curl helices to other closely spaced helices will result in a total material bridging and a less-than-optimal uniform secondary wall thickening, or no added shear resistance for example, the only possible solution is to add helices with an opposite curl, resulting in a net-like or reticulate pattern. This can be attained by simply bridging the spires of the already present helices. The different reinforcement design patterns are show in Fig. 5. All the secondary wall patterns reported in the literature (Fig. 2) correspond to a design solution from Fig. 5. or to a combination of two solutions.
Figure 6 shows scanning electron micrographs of the xylem of a grass stem (Elytrigia repens) where different secondary wall thickening patterns can be seen to agree qualitatively with the predictions of the model (Fig. 5). A complete set of optical photomicrographs was presented by Goodwin (1942) in his seminal work, showing all the different types of secondary wall thickening. Bierhorst and Zamora (1965) described secondary wall pattern formation and compiled observations on more than 1350 species; Meylan and Butterfield (1972) also presented scanning electron micrographs of wood xylem vessel elements, showing with exceptional detail the net-like or reticulate pattern of the secondary wall similar to Fig. 5D. Classical botany texts and publications contain a wealth of such micrographs that can be seen to follow the optimal design solutions. Note that the pitted vessel type reported in many works as the last form of reinforced xylem (Jensen and Salisbury, 1972; Ray, 1972; Neushul, 1974; Esau, 1977) (Figs 2E and 6D) corresponds to a heavily thickened reticulate pattern (Fig. 5D), where the secondary wall approaches a uniform thickening layer. The regularly staggered pits correspond to unthickened parts of the xylem with the primary wall autolysed to allow side-to-side transmission of water from one vessel to the other or to the surrounding parenchyma. Figure 7 shows schematically the evolution from the reticulate to the pitted pattern.
Theoretical predictions and experimental observations
In a given plant species it is reasonable to assume that the primary cell walls of vessels would have more or less the same average thickness, t, as well as the same average wall stress, σ*, across the existing range of diameters and lengths. Using this assumption eqn (5) suggests the need for larger reinforcement ratios, A/s, as the radius, R, or the pressure, p, increase in the xylem vessel. This prediction is verified by the observations of Goodwin (1942) and Esau (1977) who show a progression of thickening patterns, from annular to reticulate, as the radius of the vessel increases and the primary wall thickness remains more or less constant. Equation (6) provides a direct way of verifying the mechanical model against geometric measurements. t, s, A and α were estimated from scanning electron microscope observations performed in this study and from published photomicrographs of vessel sections in mostly herbaceous plants (Table 2). The dimensionless factor st/rA was then calculated and compared with theoretical predictions of eqn (6) in Fig. 8 for r = 1; the data points show the same trend as predicted by eqn (6) and agreement is relatively good. Note that the last entry in Table 2 does not show in Fig. 8 because it falls under case II where only hoop reinforcement is needed in the form of circumferential rings: the observed angle is zero as predicted by the model, st/A > 1 and eqn (6) is not applicable.
Growth, differentiation and secondary wall thickening
The secondary wall thickening pattern has been used as an index of growth and differentiation in xylem vessels (Goodwin, 1942; Wooding and Northcote, 1964; Bierhorst and Zamora, 1965; Jensen and Salisbury, 1972; Ray, 1972; Neushul, 1974; Esau, 1977; Zimmerman, 1983; Fahn, 1990; Paolillo and Rubin, 1991). The protoxylem develops and matures with the growing plant and has a smaller diameter than the metaxylem, which matures after the plant has finished its primary growth phase (Fig. 1). Having a smaller diameter, the protoxylem only needs annular and helical reinforcement while the metaxylem, functioning at the same pressure but with a larger diameter, will require more reinforcement resulting in a reticulate pattern. Putting aside hydraulic considerations, one reason why the plant only develops small diameter xylem (protoxylem) in its early growth phase is the need to allow for growing strains, which can reach as high as 13 % in a functional xylem, before the primary cell wall is ruptured and the vessel in question is rendered useless (Paolillo and Rubin, 1991; Niklas, 1992) (Figs 1 and 2). The annular and helical reinforcement patterns do not offer as much resistance to elongation as a reticulate pattern. The helical and annular reinforcement, having the lowest longitudinal stiffness among secondary wall patterns, will resist growth the least and stay functional for the longest time possible.
Concluding remarks
The constant strength design principle has been used by mechanical engineers to optimize the design of engineering pressure vessels (Harvey, 1980). Application of this principle has led to metallic pressure vessels reinforced with coil-layer, ribbon or wire-wrapping and helical corrugations, and to fibre-reinforced plastic pressure vessels manufactured with optimized helical winding patterns (Harvey, 1980; Rolston, 1990; Shevchenko et al., 1993; Contech, 1994). Here, a simple mechanical model of the xylem vessel as a pressure vessel has been utilized to show that the constant strength principle can be used to explain secondary wall thickening patterns in cell walls. Experimental observations of the geometry and relative dimensions of the reinforcement thickening agree well with the predictions of the model. Mechanical design considerations underlie many observed characteristics of the xylem vessels and tracheids, which are best described as composite hierarchical systems. Recent experimental results by Reiterer et al. (1999) and Choat et al. (2003) point to the existence of optimal orientation angles of microfibrils in the secondary wall. A modelling approach based on optimization principles similar to the one presented above for secondary cell wall deposition patterns could be used to analyse and understand these microstructural systems as well (Gibson et al., 1995).
The author acknowledges the financial support of US NSF (grants number MSS-9202202 and EID 9023692) for part of this work and the invaluable contribution of Professor Lorna Gibson of the Materials Science and Engineering Department at MIT.
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