Evaluation of ionic liquid "greenness"-cytotoxicity of ionic liquids

Ionic liquids have become a hot spot in research and application due to their own superiority. At the same time, the "risk" of ionic liquids has attracted more and more attention. The toxicity of ionic liquids varies according to the target. We have to obtain the toxicity data of ionic liquids on various organisms and cell lines, fill in the blanks of ionic liquid toxicity data and clarify the toxicity mechanism. Only in this way, can we lay the foundation for the design and synthesis of new ionic liquids that are truly non-toxic, environmentally friendly and meet functional requirements. In this experiment, 1-ethyl-3-imidazole diethyl phosphate ([Emim]DEP) was prepared by using 1-methylimidazole and triethyl phosphate as raw materials. The rat cranial anterior osteoblast cell line (MC3T3-E1) was used as the research object, using MTT method, fluorescent death-sense staining observation and flow cytometry to study the cytotoxicity of 1-allyl-3-methylimidazolium chloride ([Emim]DEP) type ionic liquid. This article aims to determine the median lethal concentration by the inhibition rate-concentration curve of [Emim]DEP type ionic liquid and measure cell survival index at this concentration.