Thin Layer Chromatography and Total Flavonoid Contents of Iler Leaves (Plectranthus Scutellarioides) Under Drought Stress Treatment

Iler (Plectranthus scutellarioides) contain secondary metabolites flavonoid that widely used in the treatment of hemorrhoids, antioxidants, and tuberculosis. The objectives of this study were to increase total flavonoid content of Plectranthus scutellarioides treated with several level of drought stress. The study was conducted at the screen house, Jumantono belong to Sebelas Maret University Surakarta, July to October 2018 and flavonoids analysis was carried out at the Center for Research and Development of Traditional Medicinal Plants and Medicines (B2P2TOOT), Central Java. The research method used a completely randomized design with 4 accessions as the first factor (coarse purple, fine purple, green, and red leaves) and drought stress watered once a day, watered every 2 days, watered every 3 days, and watered every 4 days as the second factor. The data obtained were analyzed by analysis of variance (ANOVA) followed by Duncan Test (DMRT) 5%. Total flavonoid content was estimated spectrophotometrically using AlCl₃, analysis of TLC was done by using TLC densito metric method. The highest total flavonoid content performed by fine purple leaves and watered once a day with 11.044. Coarse and fine purple leaves formed the more number in TLC with 10 spots that indicates the more active compound formed.