Abstract
The hypocotyl of Chinese kale 'Cutiaoyusun' was used as explants, the effects of the different concentrations of sucrose and different browning inhibitors (activated carbon, polyvinylpyrrolidone, and ascorbic acid) on the callus proliferation and anti-browning of Chinese kale were studied in this study. The results showed that the proliferation medium with 20 g·L−1 sucrose had the best effects on the proliferation and anti-browning of Chinese kale callus, and its proliferation rate was the highest, reaching 213.5%, and the browning rate was as low as 27.77%. In addition, adding 0.2 g·L−1 ascorbic acid to the culture medium can significantly promote callus proliferation and reduce browning rate. In this treatment, the callus had the lowest browning rate of 19.45% and the highest proliferation rate of 266.98%, and the morphology of the callus was friable. This study lays a foundation for future research in molecular biology and genetic improvement in Chinese kale.
Export citation and abstract BibTeX RIS
Content from this work may be used under the terms of the Creative Commons Attribution 3.0 licence. Any further distribution of this work must maintain attribution to the author(s) and the title of the work, journal citation and DOI.