Effect of Adding Annatto Seed Powder (Bixa orellana) and Astaxanthin to the Diet in some Physiological Traits of Broiler (Ross-308)

From February 15, 2022, until March 22, 2022, researchers from the Al-Anwar Poultry Company in Babil Governorate carried out the experiment on their areas to examine the effect of adding annatto seed powder (Bixa orellana) and astaxanthin to the diet on physiological traits of broiler, using 225 unsexed one-day-old Ross-308 chickens, Chickens were randomly assigned to one of 15 cages containing one of five treatments (totaling 45 birds in all), with three replicates of 15 birds in each cage. Here's how the experiment goes down: T1 control treatment / standard diet without addition, T2 addition powder annatto seeds (0.5 g / kg feed), T3 add powder annatto seeds (1 g / kg feed), T4 addition astaxanthin (50 mg / kg feed), T5 addition astaxanthin (100 mg / kg feed), the results showed in terms of blood cellular characteristics in relation to the number of red blood cells, we note a significant superiority (p ≤ 0.05) for the treatment T3, while the number of white blood cells improved significantly (p ≤ 0.05) for the two treatments T2 and T4, and with regard to hematocrit The blood (pcv) was significantly superior (p≤ 0.05) for the two treatments T1 and T3, as for hemoglobin trait the T1 treatment was significantly (p≤ 0.05), and the H/L ratio was significantly improved (p≤ 0.05) for the T2 treatment. with regard to blood biochemical characteristics for glucose we note a significant improvement (p≤ 0.05) for T2 treatment, as for the total protein we see a highly significant superiority (p≤ 0.01) for the treatments T2, T4 and T5, and in the form of albumin we notice a significant superiority (p≤ 0.05) for the treatment T5, and in the of globulin we notice a significant superiority (p≤ 0.05) for the two treatments T2 and T4, as for uric acid we see a significant improvement (p ≤ 0.05) for the two treatments T2 and T5. with regard to protein lipids in relation to cholesterol we see a significant improvement (p≤ 0.05) for treatments T3 and T5, and for triglycerides it improved significantly (p≤ 0.05) for treatment T2, and for high-density lipoproteins (HDL) we notice a significant superiority (p≤ 0.05) for treatment T2, and for low-density lipoproteins (LDL) we see an improvement Significant (p ≤ 0.05) for T5 treatment, and very low density lipoproteins (VLDL) improved significantly (p ≤ 0.05) for T2 treatment. As for the indicators of oxidative stress in the blood, we see a significant improvement (p≤ 0.05) for the (AST) enzyme for T3 treatment, and for the (ALT) enzyme we notice a significant improvement (p≤ 0.05) for the T4 treatment, while for the (GPX) enzyme we see a highly significant superiority (p≤ 0.01) for the T2 and T4 treatments, and for the Heat shock protein (HSP 70) We notice a significant improvement (p ≤ 0.05) for the two treatments T3 and T4.