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Detection of aflatoxin M1 in powdered milk and sweetened condensed milk products in several cities in Java with HPLC-fluorescence method

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Published under licence by IOP Publishing Ltd
, , Citation H Wijaya et al 2018 IOP Conf. Ser.: Earth Environ. Sci. 102 012028 DOI 10.1088/1755-1315/102/1/012028

1755-1315/102/1/012028

Abstract

Aflatoxin M1 (AFM1) is a hydroxylated metabolite of aflatoxin B1 (AFB1) produced by lactating animals due to consuming AFB1-contaminated feed. AFM1 can be found in dairy products because it is resistant to heat during processing. This study aimed to detect AFM1 in powdered milk and sweetened condensed milk sold in several cities in Java. The amount of powdered milk sample was 20, while the amount of sweetened condensed milk sample was 16. AFM1 detection in powdered milk and sweetened condensed milk was conducted by HPLC-fluorescence method. The results showed that the concentration of AFM1 in powdered milk ranged from undetectable to 0.549 μg/kg and the highest data (55%) was distributed in concentration range of >0.05 μg/kg - 0.2 μg/kg. On the other hand, AFM1 levels in sweetened condensed milk ranged from undetectable to 0.056 μg/kg and 43.75% data was distributed in concentration range of >0.025 μg/kg - 0.05 μg/kg. All powdered milk and sweetened condensed milk samples have met the maximum level of AFM1 according to Indonesian regulation.

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10.1088/1755-1315/102/1/012028