Preparation of silver nanoparticle by green synthesis and application to leather in enhancements of antimicrobial activity of leather

The objectives of this study were to prepare nanoparticle by using extract of Tectona grandis L. leaf and to deposit nanoparticle on goat leather via ex situ method, and also to characterize the antimicrobial activity of modified goat leather. The stages in this study were preparing of silver nanoparticles via green synthesis method by using extract of teak leaf, characterizing of nanoparticle by using a UV-Vis spectrophotometer and Particle Size Analyzer (PSA), impregnating the nanoparticle on the pickle leather by ex situ method, and analysing the antibacterial and antifungal activities of modified leather by the diffusion method. The silver nanoparticles were greenish brown in colour, having absorption at the wavelengths of 449 nm which indicator the formation of silver nanoparticles. The silver nanoparticles have an average particle size of 109.9 nm. The results of antimicrobial tests against the bacteria Staphylococcus epidermidis DNCC 6018, Escherichia coli FNCC 0047, and the fungus Candida albicans ANCC 0048 showed that all variations of the leather have different abilities in inhibiting the growth of the bacteria and the fungi. The pickle leathers after modification with nanoparticle Ag showed the highest antibacterial activities against Staphylococcus epidermidis DNCC 6018 and Escherichia coli FNCC 0047.