Abstract
In order to obtain microbes producing microbial oil from environment, a strain, marked as TC14 was isolated by replica plating and Sudan Black staining on the selected media. Its colony is smooth; milk white, slightly transparent, round edge, sticky. Its cell is long-rod, G-, intracellular lipid granule. The results of physiological and biochemical research: catalase, denitrification and fluorescent pigment tests are negative; metachromatic granule staining, amylolysis, lecithinase tests are positive. According to its sequencing result of 16S rDNA, strain TC14 is classified and belonged to Bacillus, namely sp. TC14. The optimum inoculation temperature is 35°C and the most suitable initial pH value is 10. The intracellular lipid of strain TC14 is extracted by the way of hydrochloric acid hydrolysis and the calculated ratio of oil-production is 5.08%.
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