The effect of molase and urea on the production of alkaline protease from indigenous Aspergillus flavus DUCC K225

Protease is one of industrial important enzyme which can be produced by microorganisms. including fungi. Alkaline protease had physiological and commercially important. this enzyme used in detergent industries as additives. The indigenous mold Aspergillus flavus DUCC K225 isolated from lime soil of Madura island demonstrate the ability in producing extracellular protease at pH 8. The aim of this study was to examined the production of alkaline protease from A. flavus K225 on Czapeks Dox modified liquid medium containing molase as C-source and urea as N-source. The study were done in submerged culture on rotary shaker. The production of alkaline protease were observed after 7 days incubation. by examined fungal biomass. protease activity. protein content and protease specific activity. The examination of enzyme activity conducted at pH 8.5 done on crude enzyme and ammonium phosphate fractions of 0-20%. 20-40%. 40-60%. 60-80% and 80-100%. The results showed that the fungal biomass gained was 14.27 g/L. while the highest protease specific activity found at 40-60% ammonium sulphate fractination (71.75 U/ml) and 4.2 purification fold.