Prony-Householder method applied to ³¹ P NMR signals: II. Study of conjugates of ara-AMP with lactosaminated albumin

For pt.I see ibid., vol.37, no.12, p. 2213-30 (1992). Conjugates of 9- beta -D-arabinofuranosyladenine 5'-monophosphate (ara-AMP) with lactosaminated albumin (L-SA), obtain by using two different coupling procedures, produce antibodies in rats and mice which display only a small cross-reactivity, ³¹P NMR signals from the two conjugates have been examined to clarify whether different lysine and histidine residues are involved in the two reaction pathways. The occurrence of different chemical shifts and linewidths between the two conjugates, as evidenced by processing the signals with the Prony-Householder method, indicates the formation of two different complexes.