Pre-dissolved Inorganic Carbon (DIC) for cultivation Chlorella sorokiniana MH923013, Coelastrella MH923011 and Coelastrella MH923012

A step to net-zero of carbon dioxide losses in the microalgae cultivation process was targeted in the current study. This research was carried out by using pre-dissolved inorganic carbon (DIC) as a source of carbon with two doses of twenty-five and fifty millilitres. C. sorokiniana MH923013, Coelastrella MH923011 and Coelastrella MH923012 strains were used in the present investigation. The experimental data emphasized the direct influence of carbonic solution on microalgal growth according to the fast adaption of algal cells and higher productivity compared to control and dilution cultures. It was observed that microalgae strains conduct a corresponding response associated with different dosing of the saturated carbonic solution. For instance, dosing of 50 ml carbon dioxide revealed fast performance to reach the stationary phase (23-25) day with clear growth improvement. In addition, 0.1633 day⁻¹ as a maximum specific growth rate in the exponential phase was recorded with this dosing. While as there was another obvious growth enhancement with supplying 25 ml CO₂ solution, but reached the stable phase after around (37-42) day from inoculation with a maximum specific growth rate 0.0987 day⁻¹. These results demonstrate the potential of CO₂ and HCO₃⁻¹ in control the CCM pathways, thus, another step in the development of the photobioreactor design.