Two stage feeding evaluation of cattle waste medium use in chlorella vulgaris culture and chlorella pyrenoidosa culture for simultaneous production of biomass

Stress in microalgae through the metabolic pathway increases the value of lipids and caratenoids. In this study, two stage feeding in Chlorella pyerenoidosa and Chlorella vulgaris are evaluated. BBM for Chlorella vulgaris and Sorrokin/Krauss Chlorella pyrenoidosa are using as standar medium which is label as A₀/B₀, cow manure Medium with fluorescence light exposure labelled as A₁/B₁, with sunlight exposure labelled as A₂/B₂ plus urea 0.1 g/l [1] with fluorescence rays exposureas A₃/B_3.v Two stage feeding is evaluated in A₄/B₄ that compose medium A₃/B₃ which is added to A₁/B₁ medium on reaching the stationary phase. Which is Chlorella pyrenoidosa is labeled as A and Chlorella vulgaris in label B. The highest accumulation of biomass in Chlorella species was seen with the addition of urea in A₃ and A₄, but it had the lowest lipid content. During the experiment with the addition of water and nutrients from cow manure, the lipid values visibly increased, but not optimally. In Chlorella pyrenoidosa and Chlorella vulgaris species which is the object of this study, the lipid values on A₁ and B₁ are higher than the control and treatment with sunlight exposure, as well as the addition of urea.