A bulk-driven, buffer-biased, gain-boosted amplifier for biomedical signal enhancement

Abstract The growing consciousness in health, biomedical research area is becoming most sought-after to develop or design medical devices by bringing biomedical researchers and clinicians to solve many health issues. Biomedical electronics is the endorsement and resolution of many techniques in engineering powered biology and medicine. The medical imaging accounts the design and development of electrophysiological monitoring devices to examine the whole human body for proper diagnosis. The biomedical signals are low amplitude, low frequency, are to be amplified in the range of millihertz to kilohertz while rejecting the dc offsets, created a tremendous demand amongst neuroscience researchers and clinicians. In this design capacitive feedback, neural signal recording amplifier is designed using folded cascode operational transconductance amplifier (OTA). The neural amplifier uses an AC coupled input signal to segregate the DC offset voltages generated at electrode-nerve interface due to chemical reaction and AC coupled input also represses the flicker noise. A large resistor, implemented using two series MOS devices as pseudo resistors, used in parallel to capacitive feedback to exploit device off-resistance for DC feedback normalization. This article presents a bulk-driven, buffer-biased, gain-boosted amplifier for biomedical signal is designed using 90 nm CMOS technology to capture signal activity. The bulk-driven folded cascode topology is used to design amplifier, which has reduced threshold voltage and supply voltage by consuming half the power of the conventional gate driven counter parts. The proposed and designed bulk-driven folded cascode operational transconductance amplifier (BD FC-OTA) achieved a gain of 81 dB, 39 nV/√ Hz is the recorded noise characteristics by consuming less power of 2.5 µW. The Wilson current mirror method has benefited the amplifier to enhance the gain and compensation capacitor with series resistor realized by MOSFET device is used in designing 1V powered buffer biased BD FC-OTA for biomedical applications. The layout of a proposed bulk-driven folded cascode amplifier is designed using Cadence Virtuoso has a dimensions of 17.255× 35.5μm.


PUBLIC INTEREST STATEMENT
The growing consciousness in health, biomedical research area is becoming most sought-after to develop or design medical devices by bringing biomedical researchers and clinicians to solve many health issues. This research article depicts the design of low power and high gain amplifier to amplify the collected vital information from patients using neural recording systems. The proposed research work can also solve and help non-government and government institutions to ameliorate health coteries and individuals in making a spotlight on innovative and electrifying research area of biomedical electronics. We designed a high gain low noise amplifier for recording the neural signals from human body. This high gain-boosted amplifier can be used in designing and developing neural recording systems like EEG, ECG, ECoG, ENG etc. This research work mainly aims to instigate amplifiers which are the core unit to capture electrophysiological signals for all biomedical wearable devices to communicate and to solve set of impediments regarding patient monitoring in benefit of humanity.
resistor, implemented using two series MOS devices as pseudo resistors, used in parallel to capacitive feedback to exploit device off-resistance for DC feedback normalization. This article presents a bulk-driven, buffer-biased, gain-boosted amplifier for biomedical signal is designed using 90 nm CMOS technology to capture signal activity. The bulkdriven folded cascode topology is used to design amplifier, which has reduced threshold voltage and supply voltage by consuming half the power of the conventional gate driven counter parts. The proposed and designed bulk-driven folded cascode operational transconductance amplifier (BD FC-OTA) achieved a gain of 81 dB, 39 nV/√ Hz is the recorded noise characteristics by consuming less power of 2.5 µW. The Wilson current mirror method has benefited the amplifier to enhance the gain and compensation capacitor with series resistor realized by MOSFET device is used in designing 1V powered buffer biased BD FC-OTA for biomedical applications. The layout of a proposed bulk-driven folded cascode amplifier is designed using Cadence Virtuoso has a dimensions of 17.255× 35.5μm.

Introduction
The expeditious growth of portable and miniature devices, escalated demand for denser integrated circuits (ICs) that necessitates low power and low voltage CMOS circuit designs (Raikos & Vlassis, 2010). In contemporary CMOS process, the reduction of aspect ratios (or) dimensions, necessitates dwindle of the allowable supply voltages. The development of this type of low power, low voltage ICs facilitated the interfacing between electronics and biomedical applications. Battery-maneuvered equipment in biomedical electronics engineering like hearing aids, ambulatory brain machine interface systems, insulin pumps require low voltage and low power design in submicron levels (Raj, Sharma, Jasuja, & Gard, 2010). Biomedical signals (or) physiological signals are of low frequency and small amplitude which are processed with ample large time constant (τ= RC) and passive elements like capacitors less than 5 pF. The traditional analog circuits like operational amplifiers (op-amps) require power supply voltages of leastwise magnitudes of large threshold voltages and imperative signal voltage swings of NMOS (or) PMOS devices (Zuo & Syed, 2013). Many novel techniques are needed to scale down the threshold voltage to get benefitted from devices in deep sub-micron CMOS process with counter fronting the noise immunity. The biomedical engineering field is robust in adapting the portable and low power devices to monitor patients continuously (Raj et al., 2010). The continuous and prolonged time period monitoring by biomedical devices demands the frequent small-sized non-replacement batteries and weightless machines with very low power consumption and low voltage operation. The traditional CMOS gate-driven op-amps are scant for sub-micron voltage supplies due to restricted common mode range of input signal. Various techniques are developed for low voltage and low power consumption (Carrillo, Torelli, P-Aloe, Valverde, & D-Carrillo, 2010;Sharan & Bhadauria, 2017;Sharan, Chetri, & Bhadauria, 2018) integrated circuit design without enlisting the exorbitant low threshold voltage devices such as level shifting techniques, floating gate (Rodriguez-Villegas, Yufera, & Reuda, 2004), sub threshold designs (Cotrim & Ferreira, 2012;SamadSheikhaei, 2019) and bulk-driven MOSFETs (Szczepanski, Pankiewicz, Koziel, & Wojcikowski, 2015), pseudo-differential approach (Lo & Hung, 2007) and flipped voltage approach (Carvajal et al., 2005). Among above-mentioned literature; bulk-driven technique and sub threshold operation are best suitable choice for low power, low frequency, and low voltage biomedical solicitation.
Bulk driven (BD) is a promising technique to deal low voltage, low threshold demanding compatible CMOS devices (Khateb, Biolek, Khatib, & Vávra, 2010), it also provides large voltage signal swing (Ferreira, Pimenta, & Moreno, 2007). It reduces the power consumption compared to conventional gate driven technique but has increased input-referred (IR) noise because bulk source trans-conductance (g mb ) in BD devices is 3-5 times smaller than gate driven source trans-conductance (g m ) (Sharan & Bhadauria, 2016).The designing of a low voltage, low power amplifier for the low frequency biomedical signals like EEG, ECG, EMG etc is carried by operational trans-conductance amplifier (OTA); which is impetus voltagecontrolled current source (VCCS) building block for biomedical devices with high output impedance. Biomedical signals vary in their signal frequencies of up to few kHz and amplitude ranges from 1 µV to 500 mV (Bano, Narejo, Shah, & Syed, 2018). Different design correction and efficient techniques are developed and reported in recent literatures to achieve good trans-conductance of few nA/V and also to have enhanced linear range like source degeneration technique (Worapishet & Naphaphan, 2003), input attenuation method (El Mourabit, Lu, & Pittet, 2005), adaptive biasing method (Razaei & Azhari, 2015). And other techniques like feed forward technique (Kim, Kim, & Nam, 2015), Bulk-driven technique (Moreno, Baruqui, & Petraglia, 2015), current division technique and non-linear term cancellation (Lee & Cheng, 2009), current summing method (Lee & Song, 2019) are developed and discussed to cancel OTA's third-order non-linear component, to improve linearity and control the cancellation factor and also to have improved linear range (up to ± 0.4 V) under 1V supply, respectively. The design methodology and optimization of all required parameters for a biomedical amplifier is exhibited neatly using flow charts in Figures 1 and 2. Figure 1 depicts a research methodology design process that is followed for a good successful design flow to have better knowledge in designing a better amplifier for biomedical applications. Based on these steps followed in research methodology flow, a new methodology is proposed to design a bulk-driven folded cascode trans-conductance amplifier (OTA) for biomedical applications, which is summarized in Figure 2.
In this research article, low voltage, power efficient, high gain amplifier is designed for biomedical application, which can avoid regular battery replacement in portable bio-medical devices; by working continuously for prolonged time periods. The designed amplifier have improved linearity with reduced distortion at signal output and achieved high performance gain. The bulk-driven folded cascode topology is used to design amplifier, which has reduced threshold voltage and supply voltage by consuming half the power of the conventional gate driven counter parts.

Basic human electrophysiology
Human body has an intricate network which is spread across the body and is controlled by the centers in the Brain and the Spinal cord. It is this nerve network which conveys the action commands from the control centers and in return gathers feedback information from various parts of the body via electro-chemical mechanisms. The unit of this elaborate nerve network is called a neuron. Neuron is a cell which has a cell body, dendrites, and an axon. Neurological and psychiatric disorders might be due to changes in inter-neuron information transfer and changes in the excitability of the neurons. Epilepsy is an example of a disease due to abnormal neuronal excitability. The information is transferred between neurons in the form of electrical signal which result from the flow of chemical ions across the cell membrane through ion channels. There are broadly two types of ion channels; they are leakage channels and voltage-gated channels. The leakage channels are open at rest and influence the cellular resting membrane potential. Voltage-gated channels on the other sideopen and close rapidly creating rapid signals called action potentials. These action potentials are generated near cell body and are transmitted along the axon till the nerve terminal without much decrement due to myelination of axon. At the nerve terminal, the action potential-induced depolarization opens voltage-gated calcium channels, which release chemicals called neuro-transmitters outside the neuron. When the neurotransmitter binds to another neuron it alters its excitability and thereby transferring the information, which is further propagated till its intended end site.

Resting membrane potential and properties of a passive membrane
Cell membranes are selectively permeable to K + (Potassium) ions at rest which leads to a less negative (depolarized) membrane potential and the electrical gradient driven influx is slowed down. Inside the neuron K + concentration increases to the levels of 100 mM while outside it is only 2-6 mM, this sets up a chemical gradient. These electrical and chemical gradients acting in opposite directions become equal at a specific membrane potential (for K + , it's around 90 mV). This is called the equilibrium potential or Nernst potential for K + (Sadock et al.). Its formula is E K = (RT/zF)ёln([K] o /[K] i ) where R is the ideal gas constant, T is temperature in Kelvin, z is the valence of the ion, F is the Faraday's constant, [K] o and [K] i are the K+ concentrations outside and inside the cell. However, cells are permeable to many other ions like Calcium (Ca 2 + ), Sodium (Na + ), Chloride (Cl − ) etc. Therefore, the true resting membrane potential is the average of the equilibrium potentials of various individual ions, which are flowing across the membrane in that particular cell. If we think of a cell membrane as an electrical circuit, then ion channels can be thought of as variable resistors. These channels are selective to which ions can pass through them, have a degree of conductance i.e. the relative ease with which ions pass through them. Transmembrane voltage drives ion flow through the ion channels which results in current. In this scenario, the Ohm's law can be depicted as follows: I ionic = g(V m -E rev ), where V m is the membrane potential, E rev is the equilibrium potential for the passing ions and V m -E rev is the driving force. Apart from this transmembrane current, some current must be used to change the membrane capacitance (C m ), this capacitive current is depicted as: I cap = C m (dV/dt) that is the capacitive current during changing voltage over time. The total current across a membrane at any given time is the sum of I cap and I ionic . Figure 3 depicts the distribution of various chemicals like Na + , K + , Ca 2+ , and Cland current direction across the cell membrane (Sadock et al.). These neurons using electrical signals will determine the brain's local and network properties and result from the flow of ions across cell membranes through macromolecular pores called ion channels.

Action potential and properties of membrane in active state
When neurons depolarize, Na+ (sodium) channels rapidly open and shift the membrane potential towards the equilibrium potential of sodium. As leakage channels are open at rest, there is a balance between leakage currents and Na+ channels opened at depolarization, but at a specific point, Na+ current exceeds the leakage current, membrane potential at this state is called the threshold potential. However, as Na+ depolarization opens voltage gated K+ channels; the membrane potential overshoots 0 mV and then rapidly returns to resting membrane potential after a transient undershoot. This rapid change occurs over several milliseconds and is called as an Action potential (or commonly as Sodium spike) and this propagates along the length of the axon. At the axon terminal, the spike provides Ca2+ opening depolarization and the resultant release of neurotransmitters which excite another neuron and thereby communicating information (Sadock et al.,0000). Figure 4 depicts the depolarization and repolarization wave in a nerve cell (Sadock et al.,0000).

Bulk-driven technique for bio-medical signal amplifier
Bulk-driven technique was first proposed in (Guzinski, Bialko, & Matheau, 1987); whose operation is akin to FET (Blalock, Allen, & Rincon-Mora, 1998). The channel current in BD MOSFET is attuned by changing bulk source junction potential (V BS ) when inversion layer is formed beneath the gate terminal of MOSFET due to sufficient gate-to-source biasing voltage (Zuo & Islam, 2013). The operational tans-conductance (OTA) majorly dissents in two ways from widely used amplifiers: 1. The gain of an OTA can by varied by bias current supplied. 2. The output signal in OTA is a current. Therefore, OTA is widely adopted for analog signal processing applications. The OTA design, implementation, and controlling do not require external feedback. Transistor used to design OTA Figure 3. Distribution of various chemicals like Na + , K + , Ca 2+ , and Cland current direction across the cell membrane. These neurons using electrical signals will determine the brain's local and network properties and result from the flow of ions across cell membranes through macromolecular pores called ion channels (Sadock et al.).
is a four terminal device producing output current on triggering differential voltage input. The relationship between input-output signals of OTA is given by Where V 1 , V 2 are input signals, g m is the gate trans-conductance of OTA and I out is the output current.
The operational characteristic of the BD and gate triggered NMOS devices are depicted in Figure5 and 6 (Raj et al., 2010). It is reconnoitered that gate triggered NMOS devices in active region needs minimum threshold voltage of approximately 0.375 V; whereas BD NMOS will be in active region with even zero input biasing voltage at bulk of device, which behaves as depleted NMOSMOSFET. In BD technique; input voltage signal is applied to the bulk of input devices. BD devices are operated at low supply voltage to amputate the threshold voltage from the signal path. To assist low power  . High output impedance low-voltage current mirror (Raj et al., 2010). and low voltage supply, BD is preferred over gate-triggered MOS. The linearity of an OTA without extra power-dissipation can be ameliorated by using BD transistors, which provide innate signal attenuation. The increase in linearity trade off with noise, the input-referred noise is increased.
The g m and g mb relationship of the BD transistors are given by Where C BC is total bulk channel capacitance and is given by Where C j is zero bias junction capacitance and is given by The output conductance (g 0 ) and output resistance (r 0 ) of BD devices are given by The unity gain frequency is given by Where C BS is bulk source capacitance and C B-sub is bulk substrate parasitic capacitance The large signal model equation of BD MOSFET is given by (Sarin Vijay & Moni, 2018) For V DS ! V DS Sat Figure 6. High-swing Wilson current mirror (Raj et al., 2010).
The BD MOSFETs' small signal bulk trans-conductance is given by:- The BD Cascode configuration is used to improve current matching in current mirror and to increase the output impedance. The BD current mirrors used to eliminate the large voltage drops in input devices.

Sub threshold device performance
The current in the weak inversion region is an exponential ramification of the input voltage.
The MOSFET drain to source current (I DS ) for the sub threshold region is given by.
Where I S is the characteristic current ⇒I S ¼ 2nμC ox V 2 T , n P is the slope factor in weak inversion, V GS , V BS , and V DS are gate, bulk, and drain voltages, respectively, V T 0 is threshold voltages at V BS = 0, V T = KT q that is thermal voltage, K is Boltzmann's constant, q is electric charge.
The transistor will enter into saturation region when The trans-conduction of the MOSFET gate terminal is given by The gate trans-conductance (g m ) of MOSFET is a function of drain current (I D ) and nKT q ; the bulk terminal trans-conductance of the transistor is given by Equation (18) depicts that g mb is a function of Fermi potential 1 F ð Þ and body effect coefficient γ ð Þ.

Noise analysis of CMOS for bulk-driven devices
The ubiquitous CMOS design is escalated the demand for fastidious low power, low noise features, which in turn increases obstruction to achieve high signal to noise ratio. The bio-medical operated signals lead to increase in noise on reducing the device size.
The dominant sources of CMOS device noise are to be superintended to design front end amplifiers for bio-medical applications. The dominant noise sources are categorized as (i) Flicker Noise, (ii) Shot Noise, (iii) Thermal Noise, (iv) Bulk-driven Noise, (v) Avalanche Noise, and (vi) Burst Noise. The spectral density, that is noise voltage (or) noise current per root hertz V ffiffi ffi

Flicker noise
It is also called as 1 f noise randomly at si À sio 2 interface and gate-oxide area. The surface currents of MOSFET are highly susceptible to interface defects which produce high 1 f noise. The flicker noise can be modelled by gate terminate MOSFET in series with voltage sources.
The 1 f noise (or) flicker noise of MOSFET is given by Where K = 10-25 V 2 F

Shot noise
This noise is resulted due to the random charge crossing across potential (or) voltage barrier. The instantaneous current is defined as composition of average large number of random current pulses. The average of shot noise is mean square value given by Where df is differential frequency, q is 1.62 x 10 -19 C and q ID is current power density in A 2 Hz .

Thermal noise
It is the noise because of MOSFET and resistors, generated by channel. The noise long channel MOSFETs in saturated region is modeled by current source between source and drain terminals. The Power Spectral density is given by Where γ ¼ 2 3

Bulk-driven noise
The bulk-driven approach is varied from the gate driven by gain factors, which implicate the channel noise at the input. The additional thermal noise is also because of bulk sheet resistance of bulk-driven devices. The Bulk noise of MOSFET is given by Where R bi is i th gate channel effective series bulk resistance, R gi i th series gate-metal effective resistance and η is g mb g m .

Avalanche noise
The Avalanche noise is produced due to the reverse break down operation of PN junction of MOSFET. The burst and Avalanche noise are less hazardous, so they can be obliterated.

Burst noise
It is mainly ascribable to the deformity in heavy ion implants and semi-conductor materials. It is also known as popcorn noise because of popping sound it makes during signal recording.
5. Bulk-driven folded cascode amplifier for bio-medical signal amplification

Circuit description
In this article, capacitive feedback neural signal recording amplifier is designed using folded cascode operational transconductance amplifier (OTA). The neural amplifier uses an AC coupled input signal to segregate the DC offset voltages generated at electrode-nerve interface due to chemical reaction and AC coupled input also represses the flicker noise. A large resistor, implemented using two series MOS devices as pseudo resistors, used in parallel to capacitive feedback to exploit device off-resistance for DC feedback normalization (Kim & Cha, 2018b). The pseudo resistor occupies smaller area by providing a large resistance. The miller capacitances (C 1 and C 2 ) are used to filter the voltage offsets between buffer-biased stage and output stage (Lee & Song, 2019). The bulk-driven technique capacitates the low voltage signal to the input terminal of amplifier, which provides the benefits of threshold voltage. The bulk-driven folded cascode topology is used to design amplifier which has reduced threshold voltage and supply voltage by  consuming half the power of the conventional gate driven counter parts. Figure 7 shows the block diagram of bulk-driven FC amplifier. It is designed with the bulk-driven FC trans-conductance amplifier using capacitive feedback topology; enabling the gain setting and here mid band gain is enumerated by the ratio of input capacitor (C 1 ) and feedback capacitor (C 2 ). Figure 8 presents the proposed bulk-driven folded cascode trans-conductance amplifier (OTA). The bulk terminal of input devices of the folded cascode amplifier is fed by biomedical low frequency signal to ease the wide input common mode signal range; to eradicate the curbs of the threshold voltage. The ground or zero voltage terminal is biased to the gate terminals of the input bulk devices to allocate required DC voltage biasing. Bulk-driven Fold Cascode (FC) amplifier has four stack transistors to achieve the high output resistance. The mismatch problem of MOS transistors threshold voltage, which may forefront the bias current disparity in the cascode arm is resolved by the negative feedback configuration in the block diagram. An offset voltage is established between the input signal terminals of trans-conductance amplifier by the negative feedback which avenues the current beck into the input terminal (Agrawal & Sankaran, 2017) branch. The low pass cut off frequency signal is adjusted at high value to reinforce the action potential signals. The sub Hertz high pass cut off signal is resolute by 1

2CFRF
; where R F is high-valued pseudo resistor for MOS device for area competence attainment Kim and Cha (2018a).
Folded cascode trans-conductance (FC-OTA) amplifier is the most widely used amplifier topology due to its large output signals swing and high gain characteristics. Large bulky input transistors are used for bulk-driven amplifier to ameliorate the recital of flicker noise and less thermal noise obtains low power consumption in input devices in weak inversion region. The greater mobility NMOS differential transistor pair is used to design the input stage of FC OTA to enhance the trans-conductance of the input device to ameliorate the gain, UGB and reduced noise characteristics. The conventional folded cascode OTA consumes high power, so input signal is triggered to the bulk of the MOS devices, which reduces the power consumption to the almost half of the conventional FC OTA topology. The continuous abate in the CMOS device technology, not reduced the threshold voltage at the pace the supply voltage has got reduced. Therefore, the input signal voltage at the gate has become equal (or) more than the supplied voltage which in turn violate the saturation conditions of MOSFET. To repress this muddle, input is applied to the bulk terminal instead of the traditional gate terminal of MOSFET. The cascading technique is used at devices to achieve high gain, by producing large output impedance (Mythry & Moni, 2018). The first stage in the amplifier design converts input voltage signal into the output current whereas overall gain of amplifier is enhanced by the gain boosting circuitry designed between the dual stage OTA circuit by coupling (or) shooting the gate terminals of the NMOS devices of the biasing circuitry. The shorted gate terminal of the PMOS devices is connected to the source of the PMOS in inverter circuit and shorted source terminal of the NMOS device is connected the drain of NMOS in inverter circuitry to achieve high gain. In Figure 8, a novel buffer-biased gain boosting circuit is introduced between differential input stage (first stage) and output stage (second stage). The buffer-biased gain boosting circuit has very low output resistance or impedance to amplify the characteristics of a BD FCOTA. The output of the first stage is applied to the CMOS buffer biasing gain boosting circuit and output of gain boosting circuitry is applied to second stage of amplifier through miller capacitor (C 1 ). Miller Capacitor is used to maintain the amplification of gain. Another capacitor (C 2 ) is also connected between gain boosting circuitry and second stage to magnify the input signal from 10 to 558 mV. The gain boosting circuit between the 2-stage amplifier design has increased the gain of design from 10 to 61.5 dB, later by adjusting the W L value of boosting circuits; gain is increased to 81.2 dB. If only miller capacitor is used instead of gain boost circuit between the 2-stage designs; gain achieved is only 10 dB.

Results and discussions
The neural signals named as action potentials (APs) are in the frequency range of 300 to 10 KHz. These small frequency signals are read by interferences like electrocardiography  Bulk-driven FC (ECG), electromyography (EMG), internal block's noise. While recording any interferences using EMG or ECG encounter back ground noise due to firing activity of electron-nerve distance and distance nerves or neurons. In order to reject unwanted interferences and to have high accuracy in recording, the neural signal recording amplifier being differential should have qualities like low power consumption, high input impedance, longer battery life, low design area, high CMRR and should have low noise to record weak signals. AC coupling DC voltage blocking scheme is employed to eliminate DC offsets by adding large series capacitor to input and also to get benefits in terms of high gain and High CMRR (SamadSheikhaei, 2019). The high gain, low noise, and lower power amplifier design requires good sizing and careful biasing of devices. The proposed bulk-driven FC OTA for bio-medical signal amplification uses current mirrors and cascode technique as the active biasing elements. The current mirrors are reasonable than passive elements like resistors to achieve low area design to provide required bias current. The cascode topology-based current mirrors furnishes wide output swing. Therefore, the folded cascode bulk-driven OTA design has wide output swing. Low power consumption and low voltage are the most vital idiosyncrasy for the biomedical signal amplification for the medical devices to operate using longer lifetime batteries. The proposed amplifier is designed by supplying 1 V, and then it is also supplied different supply voltages to check the performance of different parameters. An input signal of 10 mV is supplied to the bulk of differential inputs of the FC OTA, which has a frequency range from 1 to 10 kHz. The proposed FC OTA for bio-medical signal has achieved 81.2 dB using gain boosting circuitry (GBC) by consuming only 2.5 µW, respectively. The common mode rejection ratio of the amplifier is 113 dB. The high gain amplifier exhibited only 39:6 nV ffiffi ffi H p at 1 Hz and still less noise of 3:6 nV ffiffiffiffi Hz p at 200 Hz. Table 1 depicts the comparative analysis of proposed bulkdriven amplifier with previous literature. Table 2 gives the information about variation of different characteristics at different power supply voltages. Table 3 depicts the variation of different characteristics with respect to different temperatures between-50°C to 100°C. Figure 26 shows the characteristics extracted from designed amplifier for different supply voltages. Figure 27 gives the characteristics extracted from designed amplifier for different temperatures.

Gain characteristics
Gain of an amplifier can be defined as the difference between input signal and output signal. It is the characteristics which say about to what extent an input signal is amplified at output. It is the ratio of output signal to input signal. The amplifier gain models are depicted in Figures 9 and 10. The designed BD amplifier using bulk-driven technique has achieved a high gain of value 81 dB, shown in Figure 11. To examine the reaction of CMRR due to DC offsets, the both inputs terminals are fastened and connected to same input signal, by placing a DC offset voltage as voltage source in between two inputs. The obtained CMRR is calculated manually which is greater than 110 dB.

Power characteristics
The interference of device electronics should have very low power dissipation due to increase of harmful heat which has to be less than 1°C because of implanted interference in tissue and tissue vulnerability. And also device should consume low power because the device will be powered by wire or battery, which should have longer battery life and also easily rechargeable at shorter intervals of recharging time. The identification of electrical activity of any biomedical signal requires low power consumption for nano-sized circuits. The power consumption of BD amplifier is 2.5 μW shown in Figure 12.

IR noise
In order to design low noise amplifier for biomedical applications, transistor size and biasing are the most important parameters. To estimate the RMS noise of an amplifier, integration of bio-amplifier noise spectral density along with bandwidth is essential. Due to large time constants of integration limits, R f and C f in 1 RfCf should be considered to be close to zero. The noise displayed at the output of an amplifier is measured as a voltage. The noise is mainly produced by both current and voltage sources. The most widely used specifications for amplifier circuit noise are the IR current and IR voltage noise. These are called IR spectral density function or also called as RMS noise. The V/√Hz is required because of noise power which is added with bandwidth (Hz) or current and voltage noise density will be added with √Hz. The high gain amplifier exhibited only 39:6 nV ffiffi ffi Different noise analyses are depicted in Figure 13, 22, and 23. Figure 22 depicts the squared output noise characteristics of bulk-driven gain-boosted amplifier for biomedical applications. Figure 23 depicts the equivalent output noise characteristics of bulk-driven gain-boosted amplifier for biomedical applications.

Transient analysis
Transient analysis is usually termed or referred to time domain simulation of the amplifier design which may or may not be in steady state. The transient analysis unlike AC employs the entire large signal model to reckon the circuit behavior. The transient simulation will display: non-linearity, large signal bandwidth, slew limiting, timing issues, etc. The transient analysis is actually the critical simulation test bench for most of the circuit verification. To analyze the transient response of the bio-amplifier, a sine wave of almost 0.01 Hz with 10 mV is applied to the input terminal of proposed amplifier and obtained a magnified 555 mV sinusoidal output signal by using capacitor (C 2 ) that is connected between gain boosting circuit and second stage of design is shown in Figure 14.

Monte Carlo simulation
The Monte Carlo simulation method (MCSM) is a statistical method which is used in stochastic simulations along with many applications in areas such as physics, biology, and mathematics. The MCSM is used for a long time to procure numerical approximations of complex functions. This MCSM is typically drawn in engendering observations of some probability distribution and using the obtained sample to approximate the interested function. In this design Monte Carlo parametric simulation is done to evaluate the effect of DC gain, noise, power, transient analysis response, and phase parametric analysis due to mismatches with respect to temperature and capacitance (Figures 15-21, 24). Figure 15 depicts the Monte Carlo Simulation of gain characteristics with respect to temperature ranging from −50 to 100°C. Figure 16 depicts the Monte Carlo Simulation of gain characteristics with respect to load capacitances ranging from 1 to1pF. Figure 17 depicts the Monte Carlo Simulation of noise characteristics with respect to temperature ranging from −50 to 100°C. Figure 18 depicts the Monte Carlo Simulation of noise characteristics load capacitances ranging from 1 fF to 1 pF. Figure 19        depicts the Monte Carlo Simulation of power characteristics with respect to load capacitance ranging from 1 fF to1 pF. Figure 20 depicts the Monte Carlo Simulation of power characteristics with respect to temperature ranging from −50 to100°C. Figure 21 depicts the Monte Carlo Simulation of transient analysis with respect to load capacitance ranging from 1 fF to 1 pF. Figure 24 depicts the Monte Carlo phase-parametric response with respect to temperature ranging from −50 to 100°C.

Layout
Layout is a three dimensional design of an electronic circuit design with many different elements and interconnections. It is a representation of a circuit design in terms of geometric structures patterned in semiconductor design layers usually referred as topography. The layout of a proposed bulk-driven folded cascode amplifier is designed using Cadence Virtuoso has a dimensions of 17.255 × 35.5 μm (Figure 25).

Conclusion
The medical imaging accounts the design and development of electrophysiological monitoring devices to examine the whole human body for proper diagnosis. This article depicts the design of low power and high gain amplifier to amplify the collected vital information from patients using neural recording systems. The proposed research work can also solve and help to ameliorate health coteries and individuals in making a spotlight on innovative and electrifying research area   of biomedical electronics. We designed a high gain low noise amplifier for recording the neural signals from human body. This high gain-boosted amplifier can be used in designing and developing neural recording systems like EEG, ECG, ECoG, ENG etc. The smaller size of this proposed amplifier facilitates to develop miniaturized neural systems or biomedical telemetry. In fact, it is going to be the most promising core unit of any neural recording systems or efficient prosthetic wearable devices for electrophysiological signal monitoring because of its efficiency and smaller size. This research work mainly aims to instigate amplifiers which are the core unit to capture electrophysiological signals for all biomedical wearable devices to communicate and to solve set of impediments regarding patient monitoring from advanced health care units in benefit of humanity. A bulk-driven, buffer-biased, gain-boosted amplifier for biomedical signal is designed in 90 nm CMOS technology to capture the activity which consumes low μ power and show cased low noise characteristics. The amplifier achieved 81 dB gain by consuming 2.5 μW power. The IR noise is 39.6 nV/√ Hz for BD FC-OTA at 1 Hz. The layout of a proposed bulk-driven folded cascode amplifier is designed using Cadence Virtuoso has a dimensions of 17.255 × 35.5 μm. The designed FC-OTA can be best suitable for biomedical signal recording applications.