Mechanism of Hair Curling via Laccase-Assisted Tyrosine Grafting Using BSA as a Model Protein

ABSTRACT Commercial hair perming uses strong reducing agents and harms hair fiber’s quality even human health. In this study, tyrosine is adopted as a cross-linking agent between thiols and/or amines as the shape-changing of hair involves the breakage of disulfide bonds and the rearrangement of new bonds between keratin molecules. To investigate the mechanism of keratin cross-linking, bovine serum albumin (BSA) is used as a model protein. Disulfide bonds in BSA are successfully reduced by L-cysteine to provide free thiols. Four new cross-linked peptides are formed inter- or intra-BSA monomers, indicating that tyrosine can be adopted as a cross-linking agent not only between amines but also between thiols. Moreover, curling of Asian hair is conducted using tyrosine as a perming agent by the laccase-assisted reaction. The optimized operational conditions are hair with L-cysteine pre-treatment (50.0 mM) followed by laccase-assisted grafting with 3.0 mM tyrosine. The reshaped hair performed a better perming performance than commercial perming product before washing, although a lower perming efficiency after washing. The curling process could be accomplished without strength loss of hair fibers and with a blow-drier easily. Hence, this new methodology may lead to the development of a gentle and user-friendly approach in the hair care industry.


Introduction
The hair cosmetic industry provides us with many products to match our requirements in daily life, mainly including hair dyeing, straightening, perming, and repairing (Cruz et al. 2016; tyrosine (2.2 mM, pH = 5.0) via laccase-assisted (18 U/mL) at 40°C for 6 h with a speed of 500 rpm. Non-reacted IAM and tyrosine were removed by ultrafiltration (30 kDa), and the BSA samples were kept at 4°C for further measurement.

Laccase-assisted tyrosine grafting onto Asian hair
The hair samples (280 mg) were rolled on a glass rod, covered with L-cysteine in a solution of 20% ethanol, 15% propylene glycol, and 0.5% benzyl alcohol (pH = 10.0) for 30 min at 40°C , washed, and dried. Tyrosine solution containing 18 U/mL of laccase was added to the surface of L-cysteine pre-treated hair (rolled on a glass), followed by the blow-drying at a temperature from 25°C to 55°C for the grafting development; afterward, hair samples were dried at higher temperature (55°C −85°C). All hair samples were then removed from the glass rods and washed with deionized water.

Free thiols and disulfide bond determination
The content of free thiols was measured using the Ellman method (Hansen et al. 2007). Briefly, 100 μL of 4 mg/mL Ellman's reagent was added into 5 mL of BSA samples (1 mg/mL) or 5 mL of phosphate buffer (pH = 8.0) with 10 mg of hair, respectively. The absorbance was detected at 412 nm, and the amount of free thiol groups was calculated from the calibration curve measured using L-cysteine. The number of disulfide bonds in BSA samples was calculated by the differences in free thiol contents present before and after sodium borohydride reduction .

Molecular weight of BSA samples
The molecular weight distribution of BSA samples was measured by SDS-PAGE using 12.5% separating Tris-HCl gels on mini-protein tetra electrophoresis (Bio-Rad Laboratories, Inc., Hercules, USA). The BSA samples (0.1 mg/ml) were mixed with 4× Laemmli sample buffer (containing 10% of β-mercaptoethanol) at 3:1 (v: v) and then heated at 100°C for 5 min. Afterward, 10 μL of samples and markers (10-250 kDa) was loaded onto the wells and operated at 120 v. After electrophoresis, the gel was stained with Coomassie Bright Blue R250 for 1 h and decolorized with water overnight. The Bruker UltrafleXtreme MALDI-TOF Mass Spectrometer (Bruker AXS Co., Germany) was adopted to measure the molecular mass of BSA samples. The sample/matrix (sinapic acid) mixtures (volume ratio of 1:1) were deposited on a steel target and followed by air-drying at room temperature. Then, the dried samples were conducted in the linear and positive-ion mode.

LC-MS analysis of BSA samples
Untreated BSA, Thiols-alkylated BSA, and Tyrosine-grafted BSA were hydrolyzed by trypsin (1000 U/ mL) at 37°C for 24 h followed by adding 100 μl of β-mercaptoethanol and heated in a water bath at 100°C for 10 min. Hydrolyzed samples were centrifugated at 8000 rpm for 20 min, and the obtained supernatants (containing peptides) were clarified through a 0.22 μm Whatman microfilter. All samples were confirmed by LC-MS analysis, and the collected data were analyzed using the software MassLynx V4.1. The predicted molecular masses of trypsin hydrolyzed peptides of pure BSA were provided by ExPASy resources (ExPASy 2005).

Molecular dynamics sImulation
Molecular Dynamics (MD) simulations were carried out for the BSA wild type (PDB ID: 3V03, chain A) (Majorek et al. 2012) and its designed analog presented three reduced disulfide bonds, as pointed out by experimental results. The first system was named as BSAwt, and the second as BSA-3CysSH. The modeling details of BSA systems were shown in the Supporting information. Images generated for visual inspection and data analysis were prepared using PyMOL Version 2.0.

Characterization of grafted hair
The loops and length of tyrosine-grafted hair were measured before and after washing. The perming efficiency (PE) is calculated using Equation (1).

PE %
ð Þ ¼ n o loops after treatment=length after treatment n o loops before treatment=length before treatment � 100% (1) FT-IR spectra of hair samples were detected using a Thermo Nicolet iS10 spectrometer (Thermo Fisher Scientific Co., Waltham, MA, USA). Data were collected from 4000 to 600 cm −1 , with a resolution of 8 cm −1 and 32 scans.
Mechanical properties of hair were evaluated by Young's modulus and breaking strength. For each sample set, 20 single hairs were mounted in the tensile jig with a fixed gauge length of 25 cm and a constant rate of 250 mm/min until breakage on an Instron 5565A tensile equipment. All the samples were kept under the same temperature (±25 ºC) and humidity (65%) before testing.

Molecular weight and reactive residues of BSA
As shown in Figure 2, BSA and BSA(cys) present bands at around 66 KDa, revealing that cleavage of disulfide bonds would not influence the molecular weight of BSA. When tyrosine and laccase are mixed with BSA(cys), the proteinic band at 66 KDa is wider and darker, indicating that tyrosine may graft on BSA(cys). However, this result does not convince the success of tyrosine-grafted BSA formation, so the MALDI-TOF analysis is conducted for quantification. The laccase shows a light color band at about 80 kDa, while the laccase band is not detected in the sample Tyrosine-grafted BSA due to a low amount of laccase after dilution.
BSA monomer presents a peak at 66,516 m/z ( Figure 3) and contains 2.40 μM/mg of free thiols and 133.23 μM/mg of disulfide bonds (Table 1), corresponding to one free thiol and 17 disulfide bonds (PDBsum 2012), respectively. A significant enhancement of free thiols is found in L-cysteine pretreated BSA (32.97 μM/mg), resulting from the breakage of disulfide bonds. After IAM alkylating, free thiols are hardly detected, revealing that all free thiols in BSA(cys) are linked with -CH 2 CONH 2 groups (Mw = 58) in IAM via C-S bonds. The molecular weight of sample thiols-alkylated BSA increases by 424 m/z, indicating that BSA(cys) contains seven free thiols caused by reduction of three disulfide bonds in BSA. Therefore, the band of sample thiols-alkylated BSA does not show significant migration to a higher molecular mass ( Figure 2). As shown in Figure 3, about nine tyrosines are successfully grafted onto BSA(cys) via laccase-assisted with mass increasing by 1546 m/z. In the   tyrosine grafting process, 13.12 μM/mg of free thiols (Table 1), corresponding to three thiol groups in BSA(cys), react with tyrosine. Meanwhile, the remaining four thiol groups re-form disulfide bonds, leading to the recovery of the disulfide bond content (122.51 μM/mg). Besides, tyrosine can also be linked to amines in BSA(cys). LC-MS of the peptides obtained by trypsin hydrolysis can provide the specific reactive cysteine residues in BSA. For sample thiols-alkylated BSA (Table 2), six peptides perform different masses, corresponding to the breakage of three disulfide bonds by L-cystine, which is consistent with the results of MALDI-TOF analysis. Observing the predicted molecular mass and peptide sequence of pure BSA (ExPASy 2005), six peptides are attributed to peptide sequence ETYGDMADCCEK (T1, 1364), NECFLSHK (T2, 977), YNGVFQECCQAEDK (T3, 1633), GACLLPK (T4, 701), DVCK (T5, 464), and EYEATLEECCAK (T6, 1388), respectively. According to the structure of BSA (PDBsum 2012), the three broken disulfide bonds are assigned with Cys-90 and Cys-101 in disulfide bond 3, Cys-167 and Cys-176 in disulfide bond 5, Cys-315 and Cys-360 in disulfide bond 10.
Based on previous results (Li, Su, and Cavaco-Paulo 2021), tyrosine was grafted onto BSA by laccaseassisted via two paths. First, tyrosine was oxidized to monomer radical by laccase and then reacted with BSA to form BSA-tyrosine conjugate via Michael addition or Schiff base. Secondly, tyrosine was oxidized to dimers (mainly dityrosine) and dimer radicals, which may promote the cross-linking of BSA between amines.
Therefore, we assume that the formation of BSA(cys)-tyrosine conjugate is assembled by tyrosine monomer and BSA(cys). As listed in Table 3, for sample Tyrosine-grafted BSA, mass changes are detected in nine peptides (T2, T4, T5, T7, T8, T9, T10, T11, and T12). The Schiff base raises a molecular weight by 163 (without extra ions) of peptides T7, T8, and T9. Whereas tyrosines link to peptides T2, T4, T5, T10, T11, and T12 via Maillard addition, corresponding to molecular weight differences of 179 (without extra ions). The disulfide bond content in tyrosine-grafted BSA decreases by 13.12 μM/mg (assigned with three thiols), so we can confirm that tyrosines are grafted onto cysteine residues in peptides T2, T4, and T5 via C-S bond.
As only dimer tyrosine was formed by laccase-catalyzed in the absence or presence of BSA (Li, Su, and Cavaco-Paulo 2021), we may exclude the influence of other tyrosine oligomers on the BSA grafting system. In total, the LC-MS analysis provides four cross-linked peptides in sample Tyrosinegrafted BSA (Table 4). Dityrosine reacts as a "bridge" between peptides (T2 and T5, T4 and T8, T9 and [T12+Tyrosine+H] + ǂ The reactive residues (Lys-535 or Lys-537) in HKPK cannot identify. Na + ion was from acetate buffer. T10, and T11 and T12), leading to a molecular weight increase of 358 m/z (without extra ions). The reactive residues are Cys-101 in T2, Cys-315 in T5, Cys-176 in T4, Lys-396 in T8, Lys-20 in T9, Lys-261 in T10, Lys-535 or Lys-537 in T11, and Lys-362 in T12. Hence, dityrosine can be adopted as a crosslinking agent not only between amines but also between thiols. MD simulations were used to evaluate the overall stability of BSAwt and BSA-3CysSH monomers. Both Root Mean Square Deviation (RMSD) and the Dictionary of Secondary Structure of Proteins (DSSP) analysis ( Figure 4) indicate that the two systems are very stable, maintaining a similar content of secondary structure (SS) to the one found in the X-ray structure (3V03) (Figure 4e). When the three disulfide bonds are reduced, a discretely smaller number of residues in helical conformation are found. Regarding the backbone and Cys RMSD values, an average value of around 0.4-0.55 nm is computed, and it seems that BSAwt deviates more from the experimental structure than its BSA-3CysSH analog. However, looking at Figure 4, panels A-C, it is easy to conclude that RMSD values are due to the displacement of the loops and helices, but any unfolding is observed. Importantly, a great alignment is noticed when both modeled BSAs are superimposed (C). Panel D highlights the disulfide bond 5, linked and not linked, revealing that little perturbation is caused by the S-S reduction.

Hair shape changes
Hair fibers are composed of a hierarchical structure, and their amorphous region consists of sulfurrich cysteine residues (Wagner and Joekes 2005). Disulfide bonds would form between two different   in HKPK cannot identify. NH4 + ion was from elution solvents of LC-MS measurement and Na+ ion was from acetate buffer. In panel e, average RMSD (nm), using backbone fitting, and average no. of residues in a Secondary Structure (SS) in comparison to the experimental BSA. The color scheme presents gray for X-ray conformation, green for BSAwt and cyan for BSA-3Cyssh. cysteine residues in a single chain or between different peptide chains, which are responsible for protecting the stability of hair fibers (Liu et al. 2021). To change the shape of the hair, disulfide bonds are disrupted using L-cysteine, and tyrosine is used as the cross-linking agent between thiols.
The perming efficiency (PE) of tyrosine-grafted hair is compared with PE of commercial products (chemical treatment), control, and tyrosine-absorbed samples ( Figure 5). All tyrosine-grafted hair presents higher PE values than that measured for a commercial perming product before washing. However, only the sample which is pre-treated by 50.0 mM of L-cysteine holds the new shape after washing with PE of 55%-65% for all of the tested tyrosine concentrations. The best performance of hair curly formation and shape maintenance is accomplished by 50.0 mM of L-cysteine pre-treatment and 3.0 mM of tyrosine grafting via laccase-assisted.
The commercial perming product keeps an excellent perming performance with its efficiency of almost 100% throughout washing procedures . Observing Table 5, among the biological hair curling measurements, tyrosine grafting performs the second PE of 65% only to BSA coating (92%, ). In addition, tyrosine grafting can be conducted with a blow-drier at a relatively lower temperature (<85°C) and less time-consuming (about 45 min). The capacity of tyrosine to change and maintain hair curls can avoid using harsh chemicals, which negatively affect hair fiber properties, consumers' health, and the environment. Therefore, adopting tyrosine as a hair curling agent is maneuverable and user-friendly in the hair industry.
FTIR spectra of raw, control, and tyrosine-grafted hair samples are depicted in Figure 6. The typical bands of amide I (1630 cm −1 ), amide II (1520 cm −1 ), and amide III (1230 cm −1 ) appear in all hair samples. After grafting (line 5, 6), the phenolic hydroxyl groups in tyrosine are present at 3201 cm −1 , and the stretching of C-O (1330 cm −1 ) in tyrosine and the typical tyrosine residue band (1042 cm −1 ) are increased (Navarro et al. 2018). Also, peaks at 1586 cm −1 , 840 cm −1 , and 795 cm −1 are caused by the changing of the aromatic structures in tyrosine (Shu et al. 2020). An additional peak at 649 cm −1 is attributed to C-S stretching , revealing the tyrosine links to thiols in hair via laccase-assisted grafting, which results in a decline of the free thiol content to about 0.5 μM/mg ( Table 6). All results prove that tyrosine is successfully grafted onto curly hair. The mechanism of hair curling is described in Figure 7. The breakage of disulfide bonds and the rearrangement of new bonds between keratin molecules are the core technologies in hair modeling. L-cysteine pre-treatment plays a role in the disruption of disulfide bonds, and tyrosine acts as a crosslinking agent between thiols of cysteine residues in hair keratin. For the BSA grafting system, dityrosine is proved to be linked to two different thiol groups directly by LC-MS analysis. Tri-, tetra-, and oligo-tyrosine, even poly-tyrosine, may perform the same reaction (Li et al. 2022). the perming efficiency is evaluated before washing. Figure 6. FTIR spectra of hair samples: 1) untreated, Raw; 2) 50 mM L-cysteine pre-treated, CYS50.0; 3) treated with buffer, Control (before washing); 4) treated with buffer, Control (after washing); 5) L-cysteine pre-treatment (50 mM) followed by laccase-assisted tyrosine grafting (3.0 mM), CYS50.0-TYR3.0 + Lac (before washing), 6) L-cysteine pre-treatment (50 mM) followed by laccase-assisted tyrosine grafting (3.0 mM), CYS50.0-TYR3.0 + Lac (after washing). Disulfide cross-linking impacts hair's mechanical properties, especially Young's modulus (Robbins 2012). Increases of Young's modulus and breaking strength are found in tyrosinegrafted hair (Table 6), due to the formation of new cross-linkages (S-dityrosine-S, S-dityrosine-NH, and NH-dityrosine-NH) between hair keratins. Tyrosine-grafted hair behaves with higher stiffness, having similar results with peptide coating methods used in hair reshaping (Malinauskyte et al. 2021;. All hair samples show a decline in breaking strength after washing. Whereas the loss of breaking strength of tyrosine-grafted hair is not so pronounced compared to raw hair, which is consistent with previous results . The establishment of new bonds plays a role in maintaining not only a new shape but also the structural integrity of the hair.

Conclusions
The curling formation of Asian hair is conducted using tyrosine as a cross-linking agent between thiols in L-cysteine pre-treated hair for the first time. The best operational conditions for the Asian hair modeling are conducted with L-cysteine pre-treatment (50.0 mM) and then grafting with tyrosine (3.0 mM), using laccase (18 U/mL) as a catalyst assisted with a blowdrier. The reshaped hair shows higher perming efficiency than commercial products before washing, while a lower curly performance after the washing procedure. The tyrosine grafting method has several advantages since no hash chemical agents are used, requiring a lower temperature and less time. Furthermore, the mechanical properties of grafted hair are performed without compromising since the new cross-linked bonds are established between tyrosine and hair. Moreover, the cross-linking of L-cysteine pre-treated hair is evidenced by using BSA as a protein model. Tyrosine can be used as a cross-linking agent between thiols and/or amines in BSA or hair.
Therefore, this new hair perming method promoted, L-cysteine pre-treatment followed by laccaseassisted tyrosine grafting, would be applied as an alternative approach to commercial hair perming. Tyrosine demonstrates a high potential to reshape keratin fibers and can be used as a "green" hair perming agent. The developed route herein presented may open a mild and maneuverable way to change the shape of keratin fibers.