New cicada fossils from Australia (Hemiptera: Cicadoidea: Cicadidae) with remarkably detailed wing surface nanostructure

Abstract Australia hosts the richest diversity of extant cicadas in the world, but a scarcity of fossils means that little is known about their evolutionary history on the continent. Here we describe the first fossilized Cicadidae from Australia. Laopsaltria ferruginosa gen. et sp. nov., Burbungoides gulgongensis gen. et sp. nov. and Tithopsaltria titan gen. et sp. nov. were all found at McGraths Flat near Gulgong, New South Wales, a recently discovered Miocene Lagerstätte. These cicada fossils preserve remarkable detail, including setae and wing membrane surface structures. Wing size varies considerably between the three newly described species and, in T. titan sp. nov., reaches a maximum size beyond what is known from extant Australian cicadas (or any other known cicada fossil), indicating a disparate cicada fauna in Australia’s Miocene rainforests. Max Moulds [ msmoulds@gmail.com ], Australian Museum Research Institute, 1 William Street, Sydney, New South Wales 2010, Australia. Michael Frese [ michael.frese@canberra.edu.au ], Faculty of Science and Technology, University of Canberra, Bruce, Australian Capital Territory 2601, Australia; Australian Museum Research Institute, 1 William Street, Sydney, New South Wales 2010, Australia; Commonwealth Scientific and Industrial Research Organisation, Health and Biosecurity, Black Mountain, Australian Capital Territory 2601, Australia. M. R. McCurry [ matthew.mccurry@australian.museum ], Australian Museum Research Institute, 1 William Street, Sydney, New South Wales 2010, Australia; Earth and Sustainability Science Research Centre, School of BEES, The University of New South Wales, Sydney, New South Wales 2052, Australia; Paleobiology, National Museum of Natural History, Smithsonian Institution, Washington, District of Columbia 20560, USA.

The fossil record of the Cicadidae (true or singing cicadas) is even more limited, with only one specimen known from the Southern Hemisphere: Fonsecacicada mineira Martins-Neto & Mendes, 2002 from the upper Eocene (Priabonian) Fonseca Formation of Brazil. Without an Australian record for the Cicadidae, it has been impossible to track how the family has changed over geological time.
The hot and warm climate of the early to middle Miocene facilitated the existence of a mesic biome throughout much of the Australian continent, a stark contrast to the dry shrublands, forests and deserts of today (Byrne et al. 2008, Christophel 1989. The recent discovery of cicada fossils from the McGraths Flat Lagerst€ atte in central New South Wales (McCurry et al. 2022) dating to the Miocene (16-11 Ma, based on palynology) provides our first glimpse into what the cicada fauna was like in these ancient environments.
Here we record the first fossilized Cicadidae from Australia, three new species in three new genera, two species belonging to the subfamily Cicadinae (tentatively identified as aff. Psaltoda in McCurry et al. 2022, Table S4), and one to the Cicadettinae, including the largest known fossilized cicada with a wingspan estimated at 165 mm. Some specimens show a remarkably detailed wing nanostructure never before found in fossilized cicadas.

Materials and methods
Four fossil cicada specimens were collected from McGraths Flat during two separate expeditions between 2019 and 2021 ( Table 1). The fossil specimens were prepared where necessary using an airscribe (ME-9100, PaleoTools, Brigham City, UT, USA). A pinned specimen of the extant species Burbunga hillieri (Distant, 1907), used for comparative imaging (Australian Museum registration No. K.537240), was collected near Talyealye Homestead, New South Wales (29 05 0 28ʺ S; 144 27 0 59ʺ E) in January, 1999. All fossil and extant specimens were photographed using a Canon EOS 7 D Mark II camera mounted on a BK Plus imaging system (Dun Inc., Charlottesville, VA, USA). Selected fossil specimens were imaged using a FEI Quanta 650 F field-emission scanning electron microscope (FESEM) (FEI Company, Hillsboro, OR, USA). The FEI microscope was operated at 15 kV, with a spot size of 2.45, an aperture setting of 5, and working distances of about 10-15 mm. Of note, the high conductivity of the rocks from McGraths Flat allowed high-resolution SEM imaging without coating. An uncoated extant B. hillieri wing was imaged using a Zeiss Crossbeam 550 FIB/FESEM (Carl Zeiss AG, Oberkochen, Baden, W€ urttemberg, Germany) operated at 0.5 kV, 10-pA probe current and a working distance of 2 mm. Photos were processed using Adobe Photoshop and Illustrator. All specimens are accessioned in the palaeontology collection of the Australian Museum (AM), Sydney, Australia. Wing terminology used in the descriptions (Figs 1-3) follows that of Moulds (2005Moulds ( , 2012. CICADOMORPHA Evans, 2009CICADOIDEA Westwood, 1840CICADIDAE Latreille, 1802CICADETTINAE Buckton, 1890CICADETTINI, Buckton, 1890 Laopsaltria gen. nov.
Diagnosis. Forewing with eight apical cells; ulnar cell 3 angled to radial cell; veins M and CuA meeting basal cell wide apart, the departure of CuA far more proximal than M; vein RP gently curved; vein CuA strongly bowed along its length; crossveins r and r-m far apart, far more than the length of those crossveins; crossvein r similarly angled to crossvein m; apical cell 2 much expanded towards wing margin; ulnar cell 1 not markedly tapering; wing margin fully formed, narrow but not contiguous with ambient vein; costal margin narrowly ampliate to node; wing tending to be rounded in shape with a likely broadly rounded apex.
Differs from all other documented genera in the following character state combination: M and CuA widely separated at basal cell, CuA strongly bowed; RP gently curved; crossveins r and r-m far apart (far more than length of crossveins), and crossveins r and r-m misaligned making r instead similarly aligned with crossvein m.
Etymology. From the Greek genitive 'laos' meaning rock or stone and referring to the fossil status of the specimen. Feminine.
Laopsaltria ferruginosa sp. nov. (Figs 1, 4E, 5A-C, 6D-F) Diagnosis. Differs from the species in the closely similar genus Terepsalta Moulds, 2012 in having the three inner sections of the discal cell all of similar length (the proximal section in Terepsalta is very short); from Adelia Moulds, 2012 in having apical cell 3 very long (short in Adelia) and a costal margin gradually reducing (abruptly narrowed at the node in Adelia); and from Diemeniana Distant, 1905c in having crossveins r and r-m far apart (close together in Diemeniana) and the distal section of the discal cell as long as medial section (shorter in Diemeniana).
Holotype. AM F.147104, an almost complete forewing in ventral view together with a small part of hind wing, remnants of the other forewing, ventral thorax and an eye; part and counterpart each in two pieces (Fig. 1).
Etymology. From the Latin 'ferruginosus' meaning rust-coloured or rusty, pertaining to the reddish brown colour of the fossil caused by iron minerals. Description. Small cicada, forewing length 23 mm; the three inner sections of the discal cell all of similar length; apical cells 2-4 longer than ulnar cells 1 and 2; without infuscations. The ventral body (and the crumpled other forewing) are too decomposed to show diagnostic features.
Remarks. Laopsaltria ferruginosa appears closest to the extant genera Terepsalta, Adelia and Diemeniana. The forewing division of CuA 1 by crossvein m-cu so that the proximal section is shortest for the most part separates the Cicadettinae from the Cicadinae, the only two subfamilies of Cicadidae found in Australasia (Moulds 2005), although there are some exceptions to this character. Among extant Australian Cicadettinae, Laopsaltria is similar in several It is difficult to determine how closely related Laopsaltria is to Terepsalta, Adelia and Diemeniana as Laopsaltria does differ in other attributes as discussed above under Distinguishing features. It is possible that Laopsaltria is ancestral to the sister clades in Marshall et al. (2016) that incorporate both Terepsalta, Adelia and Diemeniana, and possibly ancestral to a large radiation of Cicadettini that is sister to Terepsalta þ Adelia þ Diemeniana and that has not only radiated extensively in Australia but is also found in fewer species through Asia, Africa, Europe and North America.
Two other extant Australian genera have attributes somewhat similar to the aforementioned genera, Kobonga Distant, 1906 andMarteena Moulds, 1986, each having M and CuA separated at the basal cell but very close together or in some Kobonga fused as one. These fall within the clade branching above TerepsaltaþAdelia þ Diemeniana and can be considered more highly derived (later branching) than Laopsaltria because of the closer proximity of M and CuA (Moulds 2005, p. 412 Burbungoides gen. nov. Type species. Burbungoides gulgongensis sp. nov.
Diagnosis. Forewing with eight apical cells; ulnar cell 3 angled to radial cell; veins M and CuA meeting basal cell wide apart, their departure with little divergence for much of their length making the medial cell unusually narrow at its proximal end; vein CuA 1 divided by crossvein at about mid length; costal margin abruptly narrowing at node; vein CuA bowed along its length; vein M 3 þ 4 curved inwards on medial cell; crossveins r and r-m far apart, far more than the length of those crossveins, and similarly angled; crossvein r similarly angled to crossvein m; crossvein m broadly S-shaped; ulnar cell 1 narrow and not markedly tapering; apical cells tending shorter than ulnar cells; wing margin fully formed; costal margin not ampliate; wing tending slender in shape. Hind wing with six apical cells; apical cells about as long as costal, radial and medial cells.
Differs from Burbunga Distant, 1905b in possessing very long hind wing apical cells (about as long as costal, radial and medial cells) and the costal margin abruptly narrowing at the node. Distinguished from all other documented genera in the following character state combination: forewing veins M and CuA meeting basal cell wide apart (and the departure of CuA probably more proximal than M); the medial cell very narrow at its proximal end; vein CuA 1 divided by crossvein at about mid length; vein CuA bowed; the costa abruptly narrowed at node; and the hind wing apical cells long, about as long as costal, radial and medial cells. Holotype. AM F.147105, an almost complete forewing missing base, and hind wing partly obscured; part and counterpart (Fig. 2).
Etymology. Named after Gulgong, the town close to the type locality of this species. Description. Forewing length 40 mm; distal section of the discal cell very much shorter than either medial or basal sections, the medial section especially long; apical cells 2-4 shorter than ulnar cells 1 and 2; infuscation overlaying proximal end of RA 2 , crossveins r and r-m and the length of RP between them, proximal part of M1, crossveins m and m-cu, and at extremities of M 1 -M 4 and CuA 1 (extremities of RA 1 and RP not visible).
Remarks. Burbungoides gulgongensis is close to the extant genus Burbunga. The forewing division of CuA 1 by crossvein m-cu so that the proximal section is longest is indicative of the subfamily Cicadinae (Moulds 2005), otherwise found only in the Tibicininae of Eurasia and North and South America but absent from Australasia and Africa. Features suggestive of tribal placement are the separation of forewing veins M and CuA at the basal cell and more significantly their departure with only minimal divergence making the proximal end of the medial cell much narrower than is usually found in cicadas, a feature accentuated by the inward curvature of M 3 þ 4 at its proximal end. Further, crossvein m is broadly S-shaped, and the apical crossveins r and r-m are far apart, much more so than the length of the crossveins themselves and these crossveins are similarly angled.
Infuscations on the forewing are a striking feature. These overlay crossveins r, r-m and m plus the venation adjoining them, in addition to crossvein m-cu, and the distal extremities of veins M 1 -M 4 and CuA 1 . Infuscations distributed like this are found in relatively few extant Australian species, indeed among New Guinea and South-East Asian cicadas, although close variants are common. Within the Australian fauna, an identical or near identical pattern is found in Burbunga, Henicopsaltria, Arenopsaltria Ashton, 1921, Macrotristria, Illyria Moulds, 1985, Psaltoda Stål, 1861, Tamasa Distant, 1905a and Diceropyga subapicalis Walker, 1868. While wing infuscations patterns of this nature are not diagnostic for genera, their presence and pattern do appear to reflect some degree of relationship.
The venation attributes mentioned above, in conjunction with the wing infuscation pattern discussed, in particular the narrow proximal end to the medial cell, are only found among the extant Australian species Burbunga gilmorei Distant, 1882 (Fig. 2), Burbunga queenslandica Moulds, 1994, Burbunga inornata Distant, 1905b and Burbunga parva Moulds, 1994. While this implies a close relationship of B.
gulgongensis with the genus Burbunga, and consequently the Burbungini, the fossil differs from all Burbunga except Burbunga hillieri (which differs in its gradually tapered costal margin) in having much longer hind wing apical cells. Consequently, we place the fossil in the Burbungini but allocate it to the new genus Burbungoides. ARENOPSALTRIINI Moulds, 2018(in Marshall et al. 2018 Tithopsaltria gen. nov.
Diagnosis. Forewing with 8 apical cells; ulnar cell 3 angled to radial cell; veins M and CuA meeting basal cell wide apart, with the departure of CuA distinctly closer to the cell base than M; vein CuA 1 divided by crossvein so that proximal portion shortest and considerably bowed; median veins M 1 -M 4 with their distal ends forming apical cells increasingly curved; vein M 3 þ 4 barely curved inwards on medial cell; crossveins r and r-m far apart, far more than the length of those crossveins, and both similarly angled; crossvein r markedly different to angle of crossvein m; crossvein m straight; ulnar cell 1 narrow and not markedly tapering; wing margin wide, fully formed; costal margin ampliate for basal quarter or so; wing of average proportions with anal angle tending to be angulate; the costal margin evenly curved, except a little more so in subapical region. Distinguished from all other documented genera in the following character state combination: forewing veins M and CuA meeting basal cell wide apart with the departure of CuA far more proximal than M; vein CuA 1 divided by crossvein so that proximal portion a little shorter than distal portion and considerably bowed; crossveins r and r-m widely separated (distance between them about twice the length of a crossvein); and in having the distal and medial sections of the discal cell both very long and much longer than the proximal one.
Etymology. Derived from the Latin 'Tithonus' (Greek 'Tithonos'), the mythical consort of Aurora, symbolic of decrepit old age and changed into a cicada, and from the Latin 'psaltria', a female harpist and a traditional ending for cicada generic names. Feminine.

Etymology.
Greek 'Titan', referring to the family of giants in Greek mythology that ruled the earth until overthrown by the Olympian gods. The name symbolizes the large size of this species, the largest known fossilized cicada. Description. Forewing length 76 mm, the largest known fossil cicada, surpassed in size only by extant species in the genus Megapomponia Boulard, 2005; distal and medial section of the discal cell both very long and each longer than the basal section; apical cells 2-4 similar in length to ulnar cells 1 and 2; without infuscations (the darkening overlaying crossvein r and distal half of RP between r and r-m is treated as a doubtful infuscation and is probably an artefact as its distribution is abnormal and it abnormally darkens the vein). Remarks. Tithopsaltria titan most closely resembles species of Psaltoda and Macrotristria, Anapsaltoda Ashton, 1921, Henicopsaltria, Thopha, Arunta andJassopsaltria Ashton, 1914 (all subfamily Cicadinae). These all share with T. titan a similar forewing shape, a broad outer margin, veins M and CuA widely separated at the basal cell, strong distal curvature of the median veins forming the apical cells, a partially ampliate costa, crossveins r and r-m widely spaced and directed at a similar angle, and crossveins m and m-cu angled similarly to each other but very differently to crossveins r and r-m. While many species in these genera also have CuA 1 curved, only some species of Psaltoda have the proximal section of CuA 1 strongly bowed as in T. titan, in particular Psaltoda magnifica Moulds, 1984 and Psaltoda flavescens Distant, 1892. Among the aforementioned genera, including Psaltoda, all have forewing veins M and CuA departing the basal cell similarly distant from its base, the one exception being Henicopsaltria (tribe Arenopsaltriini) that has CuA departing closer to the base of the basal cell than M as does T. titan, in particular Henicopsaltria eydouxii (Gu erin-M eneville, 1838). This suggests that the nearest extant genera to T. titan are Psaltoda and Henicopsaltria, or more specifically, the species previously mentioned. However, these differ slightly from T. titan by one character, in having vein CuA 1 divided by crossvein m-cu so that the proximal section is longest, not a little shorter as in T. titan. This has implications at subfamily level, its division with the proximal section shortest usually implies that a species belongs to the subfamily Cicadettinae (Moulds 2005), not to the Cicadinae as suggested by the close similarity of T. titan with Psaltoda and Henicopsaltria. This, however, is not a 'hard and fast rule' and there are exceptions. Among the 15 nov. (F.147106; identity uncertain; although the specimen is well preserved only parts of the wing base and of a leg are present, but these closely match T. titan) with numerous preserved setal bases; the arrows in F point along the length of a seta that has part of its spine exposed. extant species of Psaltoda, there are three species (Psaltoda fumipennis, Psaltoda insularis Ashton, 1914, andPsaltoda adonis Ashton, 1914) where the proximal section of CuA 1 is shorter instead of longer, and there are others where the division is about mid-length (see Moulds 1990, pls 9-11). It is reasonable to assume that Tithopsaltria is another exception in this regard, its similarities to Psaltoda, Henicopsaltria and their allies far outweighing similarity to any species in the Cicadettinae. While there are some genera in Cicadettinae, even among the Australian fauna such as Aleeta Moulds, 2003, Tryella Moulds, 2003 and Diemeniana, these show only a superficial resemblance to Tithopsaltria, because they differ markedly in wing shape, have a much narrower clavus, a much narrower wing margin, as well as a closer separation of forewing veins M and CuA, all common attributes in Cicadettinae.
While T. titan shows clear affinities with extant species of Psaltoda and Henicopsaltria, these two genera belong to different tribes: Psaltoda to the Psaltodini and Henicopsaltria to the Arenopsaltriini. The attribute associating T. titan with Psaltoda is the strong curvature of the proximal section of CuA 1 , a variable feature within the genus and not of high phylogenetic value. On the other hand, Henicopsaltria has in common with T. titan vein CuA departing the basal cell closer to the cell base than vein M. This is plesiomorphic compared to the more distal departure of CuA (Moulds 2005, p. 412). As this is more likely to indicate phylogenetic relationships within the Cicadinae, we place Tithopsaltria in  (Distant, 1907). D, Overview (whole specimen and left forewing). E, F, SEM images of the wing surface with nano-protrusions (nanopillars) that are arranged in a near-perfect hexagonal pattern. Average centre-to-centre distances between neighbouring nano-holes (197 ± 19 nm [standard deviation]; n ¼ 20) or nanopillars (178 nm ± 21 nm; n ¼ 23) are given for the fossil and the extant wing, respectively.
the tribe Arenopsaltriini, a tribe containing just two extant genera, Henicopsaltria and Arenopsaltria. Interestingly, Arenopsaltria has vein CuA departing the basal cell even more basal than in Henicopsaltria, very similar to that of T. titan.

Preservation of setae, nanostructures and other anatomical details
Fossils from McGraths Flat locality usually occur with part and counterpart components, often split through the middle, which, in the case of insect wings, means that the inside of veins or the internal cuticle membrane is exposed. For example, the holotype of Tithopsaltria titan (AM F.147103) possesses a single line of setae on the leading edge of the forewing (Fig. 4A), of which only the bases can be seen since the spines are usually hidden underneath the wing (Fig. 4B, 4C). However, a single seta on the leading wing edge in the holotype of Laopsaltria ferruginosa (AM F.107104) shows a dislodged internal base and the first 10 mm of its spine (Fig. 4D), while a seta on the leading wing edge in the holotype of Burbungoides gulgongensis (AM F.107105) preserves an almost complete spine (Fig. 4E). The spine shown in Fig.  4E has also split in half exposing details of its internal structure, with suggestions of longitudinal groves. The fine ornamental patterns seen in the dark lower half of the spine in Fig. 4E, and in parts of the spine illustrated in Fig. 4F, may represent nanoscale structures of the cuticle. Furthermore, the spine in Fig. 4F and 4G preserve a close array of setal bases, suggesting that the leg of at least one unidentified species (AM F.147106) carried a covering of very small setae, a feature not unusual on extant cicada legs.
In some cases, the McGraths Flat fossil cicadas do not split through the centre, and structures near the surface can be observed. SEM imaging of AM F.107104 revealed evidence of a regular nanostructure on the wing surface ( Fig.  5A-C). The observed holes have the near-perfect hexagonal pattern and dimensions of a 'nanopillar array', a surface nanostructure on the wings of extant cicadas. For example, the wing surface topology of the extant Australian species Burbunga hillieri is characterized by small, cone-shaped protrusions (nanopillars) that are (mostly) arranged in a hexagonal pattern with an average centre-to-centre distance of 178 nm between two pillars (Fig. 5D-F). The arrangement and dimensions of these nanopillars are very similar to the arrangement and dimensions of the nano-holes in L. ferruginosa, which likely possessed a wing topology similar to modern cicadas. The actual nanopillars on AM F.107104, however, have not survived, but were preserved as a detailed mould that formed when the iron mineral goethite precipitated around the insect. Interestingly, similar looking highfidelity casts have been generated from extant cicada wings using poly(methyl metha-crylate) (see Zhang et al. 2006), gold (see Xie et al. 2008) nickel (see Xie et al. 2017) or titanium dioxide (see Zada et al. 2017), which further supports our hypothesis that L. ferruginosa had a modern wing surface topology.
High-fidelity preservation is not restricted to setae or nanostructures on the wing surface of the McGraths Flat cicada fossils (see Fig. 6). Potential internal structures of the cuticle are observable but are difficult to interpret because the taphonomy is not completely understood (McCurry et al. 2022).

Discussion
A scarcity of fossil cicadas from the Australian continent has led to ambiguity about how the fauna has changed over time. The cicada specimens described here include an unexpected degree of morphological difference to the modern fauna. Tithopsaltria titan, with a forewing length of 76 mm, is the largest known fossilized cicada and is by far the largest known Australian cicada (Fig. 7). It is only slightly rivalled in size by extant species in the genus Megapomponia Boulard, 2005 that today inhabit Southeast Asia (Lee & Sanborn 2010). The largest extant Australian species, Thopha saccata Fabricius, 1803, has a forewing length of up to 67 mm (Moulds 2008). The association of Tithopsaltria to Psaltoda and Henicopsaltria, two genera of large species and closely allied to Thopha Amyot & Serville, 1843, suggests that T. titan represents an independent evolution of extremely large cicadas in Australia.
Wing surface nanostructures have been documented for a range of extant cicada species, including several Australian species (e.g., Kelleher et al. 2016, Pogodin et Moulds (1990). Boxplots represent the size distribution of wing length within the tribe. Tribal classification follows Marshall et al. (2018). homogeneity and in the shape, size and spacing of the nanopillars. Furthermore, Sun et al. (2012) noted that ageing and storage conditions affected the outermost 'wax' layer(s) of the epicuticle in extant species, and thus changes the shape and height of the pillars. To compare the wing nanostructure of extant specimens of different ages, and extant specimens with our fossil cicadas (in which a mould of the nanopillar array rather than the actual pillars is preserved), we measured the average centre-to-centre distance between two pillars (sometimes called the 'pitch') rather than pillar diameter and spacing. Our analysis suggests that the wing surface nanostructure of Laopsaltria ferruginosa is similar to that of an extant Australian species in the same tribe, Burbunga hillieri (Fig. 5). However, more research is warranted to determine whether nanostructure details are indeed valuable taxonomic characters, as the intra-species variation of cicada wing nanostructures has never been thoroughly investigated, and a comprehensive investigation of extant species that fully reflect the diversity of the family has not yet been performed.
A lack of well-preserved fossil material has, until now, not allowed tracing the evolution of these wing surface nanostructure in cicadas (as well as in other insects). Thus, our description of a Miocene cicada wing surface nanostructure is an important first step to close this knowledge gap. Given that fossil cicadas are not particularly rare at McGraths Flat (so far, in only 3 years of excavation, seven specimens have been discovered that represent at least three species), it is tempting to speculate that additional fossil nanostructures will be reported in time.
The surface nanostructure on cicada wings possesses a range of properties including antireflection, superhydrophobicity, self-cleaning and control of microbial adhesion/ growth. The versatility of these structures has sparked considerable interest outside the field of entomology. For example, the ability of cicada nanostructures to destroy bacteria through direct physical contact rather than chemical interactions (Ivanova et al. 2012, Jenkins et al. 2020) makes such nanostructures an attractive template for the engineering of surfaces that kill bacterial contaminants and inhibit biofilm formation. Kelleher et al. (2016) established a functional assay that can quantify and compare bactericidal properties of wing nanostructures from different cicada species. So far, however, only the nanostructures of extant cicada wings have been studied. Our discovery of the extraordinary fidelity of preservation at McGraths Flat shows that key parameters of wing nanostructures can be measured in fossil cicadas. This not only helps to elucidate the evolutionary origins of wing surface nanostructures, but also opens up the potential for examining the function of these structures in these extinct species.