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Evidence of human immunodeficiency virus type 1 infection of nestin-positive neural progenitors in archival pediatric brain tissue

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Abstract

Human immunodeficiency virus type 1 (HIV-1) central nervous system (CNS) infection in children is associated with impaired brain growth and neurodevelopmental delays. Neural progenitors are critical for neurogenesis. Human multipotential neural progenitors grown in culture are permissive for HIV-1 infection, but it is not known if infection of these cells occurs in vivo. Brain tissue from pre-highly active antiretroviral therapy (HAART) era pediatric acquired immunodeficiency syndrome (AIDS) patients was examined for evidence of HIV-1 infection of nestin-positive neural progenitors by in situ hybridization; or after laser microdissection harvest, DNA extraction, and polymerase chain reaction (PCR). HIV-1 or viral DNA was identified in nestin-positive cells in four of seven HIV-1-infected children, suggesting in vivo infection of neural progenitors.

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Authors and Affiliations

  1. Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 9000 Rockville Pike, 20892-1296, Bethesda, Maryland, USA

    Lynnae Schwartz, Diane M. P. Lawrence & Eugene O. Major

  2. Department of Neurology, Children’s National Medical Center, Washington DC, USA

    Lucy Civitello

  3. Department of Physiology and Biophysics, Georgetown University, Washington DC, USA

    Anastasie Dunn-Pirio

  4. College of Chemical and Life Sciences, University of Maryland at College Park, Maryland, USA

    Samantha Ryschkewitsch

  5. School of Natural Science, Hampshire College, Amherst, Massachusetts, USA

    Elizabeth Berry

  6. Department of Microbiology, University of Minnesota, Minneapolis, Minnesota, USA

    Winston Cavert

  7. Mayo Graduate School, Rochester, Minnesota, USA

    Nikilyn Kinzel

  8. Pediatric Oncology Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, 20892, Maryland, Bethesda, USA

    Rohan Hazra

  9. HIV and AIDS Malignancy Branch, National Cancer Institute, National Institutes of Health, 9000 Rockville Pike, 20892, Bethesda, Maryland, USA

    Lucy Civitello & Rohan Hazra

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  1. Lynnae Schwartz
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  2. Lucy Civitello
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  3. Anastasie Dunn-Pirio
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  4. Samantha Ryschkewitsch
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  5. Elizabeth Berry
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  6. Winston Cavert
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  7. Nikilyn Kinzel
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  8. Diane M. P. Lawrence
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  9. Rohan Hazra
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  10. Eugene O. Major
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Correspondence to Lynnae Schwartz.

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This work was supported through the Intramural Research Program, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland, USA.

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Schwartz, L., Civitello, L., Dunn-Pirio, A. et al. Evidence of human immunodeficiency virus type 1 infection of nestin-positive neural progenitors in archival pediatric brain tissue. Journal of NeuroVirology 13, 274–283 (2007). https://doi.org/10.1080/13550280701344975

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  • DOI: https://doi.org/10.1080/13550280701344975

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