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Effects of disodium fumarate on in vitro rumen microbial growth, methane production and fermentation of diets differing in their forage:concentrate ratio

Published online by Cambridge University Press:  08 March 2007

R. García-Martínez ,
M. J. Ranilla ,
M. L. Tejido  and
M. D. Carro
R. García-Martínez
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
M. J. Ranilla
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
M. L. Tejido
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
M. D. Carro*
Affiliation:
Departamento de Producción Animal I, Universidad de León, 24071 León, Spain
*
*Corresponding author: Dr M. D. Carro, fax +34 987 291311, email DP1MCT@UNILEON.ES
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Abstract

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The effects of disodium fumarate on microbial growth, CH4 production and fermentation of three diets differing in their forage content (800, 500 and 200 g/kg DM) by rumen micro-organisms in vitro were studied using batch cultures. Rumen contents were collected from four Merino sheep. Disodium fumarate was added to the incubation bottles to achieve final concentrations of 0, 4 and 8 mm-fumarate, and 15N was used as a microbial marker. Gas production was measured at regular intervals from 0 to 120 h of incubation. Fumarate did not affect (P>0·05) any of the measured gas production parameters. In 17 h incubations, the final pH and the production of acetate and propionate were increased linearly (P<0·001) by the addition of fumarate. Fumarate tended to increase (P=0·076) the organic matter disappearance of the diets and to decrease (P=0·079) the amount of NH3-N in the cultures. Adding fumarate to batch cultures tended (P=0·099) to decrease CH4 production, the mean values of the decrease being 5·4 %, 2·9 % and 3·8 % for the high-, medium- and low-forage diet, respectively. Fumarate tended to increase (P=0·082) rumen microbial growth for the high-forage diet, but no differences (P>0·05) were observed for the other two diets. These results indicate that the effects of fumarate on rumen fermentation depend on the nature of the incubated substrate, the high-forage diet showing the greatest response.

Keywords

FumarateRumen microbial growthMethaneBatch cultures
Type
Research Article
Information
British Journal of Nutrition , Volume 94 , Issue 1 , July 2005 , pp. 71 - 77
Copyright
Copyright © The Nutrition Society 2005

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