Summary
Micropropagation of Scabiosa caucasica cv. Caucasica Blue was achieved by culturing, separating axillary and adventitious shoots, or node sectioning on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA). The highest frequency of adventitious shoots regenerated from nodal or internodal explants and leaf blade (with or without petiole) appeared to occur on MS medium with 4.4 and 18 μM BA, respectively. Addition of 0.19 or 1.9 μM α-naphthaleneacetic acid to the BA-containing medium promoted callus formation and reduced shoot organogenesis. During micropropagation, shoot nodal explants derived from in vitro shoots cultured on MS medium supplemented with 4.4 μM BA yielded 8.9 shoots per explant within 40 d after culture initiation.
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Hosoki, T., Nojima, S. Micropropagation of Scabiosa caucasica Bieb. Cv. Caucasica blue. In Vitro Cell.Dev.Biol.-Plant 40, 482–484 (2004). https://doi.org/10.1079/IVP2004572
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DOI: https://doi.org/10.1079/IVP2004572