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Changes in the alkaline phosphatase (EC 3.1.3.1) and inorganic pyrophosphatase (EC 3.6.1.1) activities of rat tissues during magnesium deficiency. The importance of controlling feeding pattern

Published online by Cambridge University Press:  09 March 2007

B. W. Loveless  and
F. W. Heaton
B. W. Loveless
Affiliation:
Department of Biological Sciences, University of Lancaster, Lancaster LA1 4YQ
F. W. Heaton
Affiliation:
Department of Biological Sciences, University of Lancaster, Lancaster LA1 4YQ
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Abstract

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1. The adoption of a meal-eating pattern of feeding by rats altered the alkaline phosphatase (EC 3.1.3.1) activity in serum and liver. It was therefore necessary to regulate the feeding pattern of both magnesium-deficient rats and control animals receiving a Mg-adequate diet in order to study the effect of the deficiency.

2. Mg deficiency decreased the activities of alkaline phosphatase and inorganic pyro-phosphatase (EC 3.6.1.1) in serum, kidney and tibia, but increased them in spleen.

3. Addition of a standard concentration of exogenous Mg to tissue extracts usually increased the activity of corresponding enzymes from Mg-deficient and control rats by the same proportion, indicating that the main effect of the deficiency was on the amount of enzyme present rather than on the efficiency of its operation.

4. Certain quantitative differences in the response to exogenous Mg and the activity ratio, alkaline phosphatase: inorganic pyrophosphatase were found between tissues from Mg-deficient and control rats. The significance of these are discussed in relation to the association of the two enzymic activities with the same protein molecule, and the possible occurrence of isoenzymes.

Type
Papers on General Nutrition
Information
British Journal of Nutrition , Volume 36 , Issue 3 , November 1976 , pp. 487 - 495
Copyright
Copyright © The Nutrition Society 1976

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