La Autoantigen Mediates Oxidant Induced De Novo Nrf2 Protein Translation*

Nrf2 gene encodes a transcription factor that regulates the expression of a cluster of antioxidant and detoxification genes. Recent works from our laboratory indicate that oxidative stress causes rapid de novo synthesis of Nrf2 protein. We have found that 5′ Untranslated Region (5′UTR) of Nrf2 allows the mRNA to undergo an Internal Ribosomal Entry Site (IRES) mediated protein translation. Using liquid chromatography tandem MS, we have discovered that La/SSB protein bound to Nrf2 5′UTR in response to oxidative stress. In vitro RNA binding and in vivo ribonucleoprotein immunoprecipitation showed H2O2 dose and time dependent increases of La/SSB binding to Nrf2 5′UTR. La/SSB protein translocated from the nuclei to cytoplasm and distributed in the perinuclear space in cells treated with H2O2. Isolation of ribosomal fractions indicated that oxidants caused an association of La/SSB with ribosomes. Physical interaction of La/SSB with representative proteins from the small or large subunits of ribosomes was found to increase in cells responding to H2O2 treatment. Knocking down La/SSB gene with siRNA prevented Nrf2 protein elevation or Nrf2 5′UTR activation by oxidants. In contrast, overexpression of La/SSB gene was able to enhance Nrf2 5′UTR activation and Nrf2 protein increase. Our data suggest that oxidants cause nuclear export of La/SSB protein and subsequent association of La/SSB with Nrf2 5′UTR and ribosomes. These events contribute to de novo Nrf2 protein translation because of oxidative stress.

Nrf2 gene encodes a transcription factor that regulates the expression of a cluster of antioxidant and detoxification genes. Recent works from our laboratory indicate that oxidative stress causes rapid de novo synthesis of Nrf2 protein. We have found that 5 Untranslated Region (5UTR) of Nrf2 allows the mRNA to undergo an Internal Ribosomal Entry Site (IRES) mediated protein translation. Using liquid chromatography tandem MS, we have discovered that La/SSB protein bound to Nrf2 5UTR in response to oxidative stress. In vitro RNA binding and in vivo ribonucleoprotein immunoprecipitation showed H 2 O 2 dose and time dependent increases of La/SSB binding to Nrf2 5UTR. La/SSB protein translocated from the nuclei to cytoplasm and distributed in the perinuclear space in cells treated with H 2 O 2 . Isolation of ribosomal fractions indicated that oxidants caused an association of La/SSB with ribosomes. Physical interaction of La/SSB with representative proteins from the small or large subunits of ribosomes was found to increase in cells responding to H 2 O 2 treatment. Knocking down La/SSB gene with siRNA prevented Nrf2 protein elevation or Nrf2 5UTR activation by oxidants. In contrast, overexpression of La/SSB gene was able to enhance Nrf2 5UTR activation and Nrf2 protein increase. Our data suggest that oxidants cause nuclear export of La/SSB protein and subsequent association of La/SSB with Nrf2 5UTR and ribosomes. These events contribute to de novo Nrf2 protein translation because of oxidative stress. Molecular & Cellular Proteomics 11: 10.1074/mcp.M111.015032, 1-10, 2012.
Nuclear factor erythroid-2 related factor 2 (Nrf2) 1 is a transcription factor that regulates the expression of a cluster of antioxidant and detoxification genes. As a bZIP transcription factor, Nrf2 can bind and activate the antioxidant response element (ARE) in the promoters of target genes, including NA-D(P)H:Quinone Oxidoreductase 1, glutathione S-transferases, epoxide hydrolase-1, hemeoxygenase-1, superoxide dismutases, thioredoxin reductase, and peroxiredoxin 1 (1)(2)(3). Whereas Nrf2 controls the antioxidant response, oxidants are a major inducer of Nrf2 protein and activity. This paradox influences the fate of cells under oxidative stress: to survive or to die.
As a transcription factor, the activity of Nrf2 is regulated at multiple levels. It is commonly known that under normal physiological conditions, Nrf2 activity is kept low through the ubiquitin E3 ligase complex Keap1/Cul3/Rbx1 mediated sequestration and proteolytic degradation in the cytosol (4). Oxidants cause Nrf2 protein to dissociate from Keap1/Cul3/Rbx1, resulting in an inhibition of Nrf2 protein degradation (1). When Nrf2 is free from the sequester Keap1, it translocates to the nucleus where it dimerizes with sMaf, ATF4 or JunD to promote transcription of target genes containing ARE in the promoters (1,5). Recent works from our laboratory indicate that de novo protein translation comprises an important mechanism of rapid elevation of Nrf2 protein by oxidative stress (3).
Little is known about how proteins are translated under stress conditions. Whereas stress causes an overall inhibition of protein synthesis, increasing evidence suggests that genes containing an Internal Ribosomal Entry Site (IRES) in the 5Ј Untranslated Region (5ЈUTR) of mRNA can bypass the mechanism of general protein synthesis, which is dependent on 7-methyl guanine cap at the 5Ј end of mRNA. Although the sequences of IRES are not conserved between genes, a common feature of IRESs is GC rich and forms "stems and loops" secondary structures. Human Nrf2 gene encodes an mRNA strand with 555-nucleotides of 5ЈUTR containing 70% G and C. A stable secondary structure containing "stems and loops" has been predicted via Zucker's MFold algorithm (Xu et al., 2011 manuscript submitted). This suggests that Nrf2 5ЈUTR contains an IRES, allowing Nrf2 protein being translated under oxidative stress.
IRES mediated protein translation requires IRES trans-acting factors (ITAFs). When interacting with IRES, ITAFs appear to recruit eIFs and ribosomes to initiate translation (6,7). Several RNA binding proteins have been characterized as functional ITAFs, including the polypyrimidine tract binding protein (PTB), poly r(C) binding protein 1/2 (PCBP1/2), La autoantigen, Upstream of N-ras (unr), heterogeneous nuclear ribonucleoproteins C1 and C2 (hnRNPC1/C2), death associated protein 5 (DAP5), embryonic lethal abnormal vision/pro- tein HuR (ELAV/HuR), and nucleolin (8,9). One or several ITAFs can bind to an IRES to regulate protein translation. Each gene recruits its own set of ITAFs for IRES binding and translation initiation. For example although unr and PTB occupy the IRES of Apaf-1 mRNA, Bag-1 IRES interacts with PCBP and PTBs (7). The IRES of XIAP has been linked to binding of La autoantigen, hnRNPC1/2 and DAP5 (7). A recent study using proteomic approaches found four proteins: G-rich RNA sequence binding factor 1 (GRSF-1), Y-box binding protein 1 (YB-1), PTB associated splicing factor (PSF), and its binding partner p54nrb, as ITAFs for regulating translation of myc family genes (10). In this study, we report the finding of La autoantigen as an ITAF regulating Nrf2 protein translation under oxidative stress.
In Vitro Transcription-The pJet hsa Nrf2 5ЈUTR plasmid DNA (1 g) was linearized with XbaI and extracted with phenol/chloroform for in vitro transcription using MEGAscript T7 kit (Ambion) in the presence of Biotin-11-UTP (Invitrogen). The reaction was carried out at 37°C for 2 h and the product was extracted with phenol/chloroform/isoamyl alcohol. The RNA probe was precipitated with isopropanol and washed with 70% ethanol. After air-drying, the RNA pellet was resuspended in nuclease free water.
RNA Affinity Chromatography-HeLa cell lysates were used for RNA affinity chromatography as described by Cok et al. (12). Briefly, cells harvested in nucleic acids binding buffer [10 mM HEPES (pH 7.6), 5 mM MgCl 2 , 40 mM KCl, 1 mM dithiothreitol, 5% glycerol, 5 mg/ml heparin] were lysed by sonication 3 times for 5 s each on ice. After centrifugation at 14,000 rpm at 4°C to remove debris, the supernatant containing 500 g proteins was used for binding to 5 g of biotinylated RNA probe on ice for 1 h. Following binding reaction, 0.2 ml of streptavidin Sepharose beads (GE Healthcare) was added for overnight incubation at 4°C with rotation. The beads were loaded on a 2 ml centrifugation column (Pierce) and washed three times with 2 ml of 1 M NaCl in the nucleic acids binding buffer. The captured proteins were released by boiling in SDS-PAGE loading buffer and were resolved in 10% SDS-PAGE. The gel was silver-stained with a mass spectrometry compatible kit (BioRad) and bands of interest were excised for LC-MS/MS analyses. Alternatively, the proteins on the gel were transferred to PVDF membrane for Western blot.
Tandem Mass Spectrometry Coupled Liquid Chromatography (LC-MS/MS)-Excised silver-stained protein bands following SDS-PAGE were digested in gel with trypsin (10 g/ml) at 37°C overnight (13). LC-MS/MS analyses were carried out using a linear quadrupole ion trap ThermoFinnigan LTQ mass spectrometer (San Jose, CA) equipped with a Michrom Paradigm MS4 HPLC, a SpectraSystems AS3000 autosampler, and a nanoelectrospray source. Peptides were eluted from a 15 cm pulled tip capillary column (100 m I.D. ϫ 360 m O.D; 3-5 m tip opening) packed with 7 cm Vydac C18 (5 , 300Å pore size, Hesperia, CA), using a 0 -65% gradient of solvent B (98% methanol/2% water/0.5% formic acid/0.01% triflouroacetic acid) over a 60-min period at a flow rate of 350 nl/min. The LTQ electrospray positive mode spray voltage was set at 1.6 kV with the capillary temperature of 180°C. Dependent data scanning was performed by the Xcalibur version 1.4 software (14) with a default charge of 2, an isolation width of 1.5 amu, an activation amplitude of 35%, activation time of 30 msec, and a minimal signal of 100 ion counts. Global dependent data settings were as follows: reject mass width of 1.5 amu, dynamic exclusion enabled, exclusion mass width of 1.5 amu, repeat count of 1, repeat duration of 1 min, and exclusion duration of 5 min. Scan event series included one full scan with mass range 350 -2000 Da, followed by three dependent MS/MS scan of the most intense ion. Tandem MS spectra of peptides were analyzed with SEQUEST™ (version 27, rev. 12, ThermonFinnigan, San Jose, CA), a program that allows the correlation of experimental tandem MS data with theoretical spectra generated from known protein sequences (15). The peak list (dta files) for the search was generated by Bioworks 3.1. Parent peptide mass error tolerance was set at 2.0 amu and fragment ion mass tolerance was set at 1.0 amu during the search. The criteria used for a preliminary positive peptide identification are the same as previously described, namely a dCn score Ͼ 0.08, and Xcorr Ͼ1.8 for ϩ1, Xcorr Ͼ2.5 for ϩ2, and Xcorr Ͼ3.5 for ϩ3 charged peptide precursor ions (16). Peptides matching proteins of interest were confirmed by visual examination of the spectra. All spectra were searched against a human protein database downloaded from IPI October 30, 2009 (ipihuman version 3.65; http://www.ebi.ac.uk/IPI), based on semitryptic peptides between 700 -4500 amu with up to two missed cleavages. At the time of the search, the ipi human version 3.65 protein database contained 86,379 entries. The results were also validated using X!Tandem, another search engine (17) and visualized with Scaffold (version Scaffold_3_00_02, Proteome Software Inc., Portland, OR), a program that relies on various search engine results (i.e. Sequest, X!Tandem, MASCOT) and uses Bayesian statistics to reliably identify more spectra (18,19). X!Tandem identifications required at least -Log(Expect Scores) of greater than 3.0. Protein identifications were accepted if they contained at least two identified peptides. Oxidation of methionine and iodoacetamide derivative of cysteine were specified in Sequest and X!Tandem as variable modifications. The protein false discovery rate (FDR) was calculated at 0.1% by the Scaffold software using the ProteinProfit based probabilities [(sum of probabilities of proteins/sum of maximum probabilities for each protein) ϭ A; 1.00-A ϭ B * 100 ϭ FDR].
RNA-Protein Complex Immunoprecipitation and qPCR Analysis-HeLa cells were lysed on ice with nucleic acid binding buffer containing RNase and protease inhibitors. Antibodies (2 g) were first incubated with 100 l of Protein A/G Plus beads (Santa Cruz Biotechnology, Santa Cruz, CA) for 1 h at 25°C. After washing away unbound antibodies, the beads were incubated at 4°C with 500 g cell lysates for 4 h. Unbound proteins were removed by 5 times washes with nucleic acid binding buffer. RNA was extracted with Trizol (Invitrogen, Carlsbad, CA) from the beads and was converted to cDNA for real-time PCR with MMLV reverse transcriptase (Fermentas, Hanover, MD) and oligo d(T) primer. The primer sets for real-time PCR were designed with an online tool provided by IDTDNA and synthesized by Signosis as 5Ј-CAGGTTGC-CCACATTCCCAAATCA-3Ј and 5Ј-AGCAATGAAGACTGGGCTCTC-GAT-3Ј for human Nrf2 (NM_006164); 5Ј-TCGACAGTCAGCCG-CATCTTCTTT-3Ј and 5Ј-ACCAAATCCGTTGACTCCGACCTT-3Ј for human GAPDH (NM_002046.3). Nrf2 mRNA abundance was determined with SYBR Green Master Mix (Fermentas) and BioRad CFX Manager Software after normalization to GAPDH.
Transfection and Luciferase Assay-The DNA plasmid or siRNA was transfected with Fugene 6 (Roche) or LipofectAMINE 2000 (In-vitrogen) respectively. HeLa cells transfected with pRL bicistronic vector of Nrf2 5ЈUTR in 24-well plates were treated with H 2 O 2 24 h post-transfection. Luciferase activity was measured using a Dual-Luciferase Assay Kit (Promega, Madison, WI) and luminometer.
Immunocytochemistry-HeLa cells were seeded onto glass coverslips in 35-mm dishes and grown to 30% confluency. After H 2 O 2 treatment, the cells were washed twice with phosphate-buffered saline (PBS) before fixation in methanol. Following blockage with 2% bovine serum albumin in PBS, the coverslips were incubated with primary antibodies (1:400 dilution) for 2 h. The coverslips were washed 3 times with PBS before incubation with Alexa conjugated secondary antibodies (Invitrogen) for 1 h. After PBS washes, the coverslips were mounted onto a microscope slide with Anti-fade Mounting Medium containing DAPI (Invitrogen). The images were acquired with a 60X lens on an Olympus BX53 Microscope and analyzed with CellSens software from Olympus.
Isolation of Ribosomes-HeLa cells were treated with 5 g/ml cyclohexamide 10 min before harvesting. The cytoplasm was extracted with lysis buffer after cells being washed twice with cold PBS containing 0.5 g/ml cyclohexamide. The lysate was centrifuged at 400 ϫ g for 5 min to remove nuclei and cell debris. The supernatant was transferred to an Eppendorf tube and centrifuged at 14,000 ϫ g for 10 min to remove postmitochondrial fractions. The supernatant was loaded on 10 and 35% sucrose cushions, and centrifuged at 30,000 rpm with a SW41 rotor for 1.5 h at 4°C to collect the ribosomal pellet (20). For ribosomal profiling, the postmitochondrial fraction was loaded on a linear sucrose gradient (15-50% w/v) and centrifuged at 40,000 rpm with a SW40 rotor for 1.5 h at 4°C. The gradient was displaced upright with 60% sucrose and the distribution of ribosome was recorded at absorbance of OD 254 nm with a BioLogic LC System (BioRad). The fractions were collected at a flow rate of 1 ml per min. Proteins in each fraction were precipitated with 10% trichloroacetic acid.
Statistics-Two-tailed Student's t test was used when two means were compared between each other. When means from multiple groups were compared, 1-way ANOVA was used. In either case, p Յ 0.05 was considered significant.

LC-MS/MS Identification of Nrf2 5ЈUTR Binding Proteins-
LC-MS/MS based protein identification has been proven useful in identifying proteins bound to RNA. We cloned the full length human Nrf2 5ЈUTR downstream of T7 promoter for in vitro transcription to generate biotinylated RNA bait for isolation of binding proteins from HeLa cells following H 2 O 2 treatment. As shown in Fig. 1, a band corresponding to the molecular weight of 50 kDa appeared specific from H 2 O 2 treated cells. Excision of the band for LC-MS/MS analysis revealed two peptides belonging to the human La autoantigen (Accession number IPI009032, 6% sequence coverage, Figs. 1A-1C).
La autoantigen is a 52-kDa protein being targeted by antibodies in patients with Sjö gren's Syndrome, and therefore also named as Sjö gren Syndrome Antigen B (SSB). To verify La/SSB protein binding to Nrf2 5ЈUTR, we used RNA affinity chromatography with biotinylated Nrf2 5ЈUTR as a bait to pull down binding proteins for Western blot to detect La/SSB, a technique named Far Western. Fig. 2A (Fig. 2B). These results confirmed that H 2 O 2 treatment indeed caused La/SSB binding to Nrf2 5ЈUTR.
For LC-MS/MS and Far Western experiments, the binding of La/SSB from cell lysates to Nrf2 5ЈUTR occurred in vitro. To demonstrate the binding of La/SSB to Nrf2 5ЈUTR occurring in cells, we used RNA-protein complex immunoprecipitation to examine the association of Nrf2 mRNA with La/SSB protein.
With cell lysates harvested after H 2 O 2 treatment, the La/SSB antibody was able to pull down Nrf2 mRNA (Fig. 3). H 2 O 2 treatment induced a dose and time-dependent increase of Nrf2 mRNA in La/SSB immunoprecipitates (Fig. 3). The dose response and time course of Nrf2 mRNA binding to La/SSB were consistent with that of Far Western data.

La/SSB Mediates Nrf2 Translation-To address whether
La/SSB binding to Nrf2 5ЈUTR is critical for Nrf2 translation under oxidative stress, we measured Nrf2 induction by modulating the level of La/SSB protein. When siRNA was transfected into HeLa cells, it was able to reduce the level of La/SSB protein. La/SSB siRNA was able to inhibit H 2 O 2 from inducing Nrf2 protein (Fig. 4A). We cloned a construct for constitutive expression of La/SSB fused with a Green Fluorescent Protein tag. Ectopic expression of GFP-La/SSB was sufficient for increasing Nrf2 protein (Fig. 4B). Measurements of Nrf2 transcripts excluded the possibility that La/SSB siRNA or GFP-La/SSB modulated Nrf2 gene transcription (data not shown).
To demonstrate that La/SSB indeed regulated Nrf2 protein translation via Nrf2 5ЈUTR, we cloned Nrf2 5ЈUTR into a dicistronic reporter construct, a system commonly used for measurements of IRES mediated protein translation (21). Such dicistronic reporter vector contains SV40 promoter for transcription of both Renilla and Firefly luciferases, with Renilla luciferase representing 5Ј 7-methyl guanine cap dependent translation and Firefly luciferase under the control of Nrf2 5ЈUTR. The ratio of Firefly luciferase over Renilla luciferase reflects Nrf2 5ЈUTR mediated protein translation. After HeLa cells were transfected with the dicistronic construct and treated with H 2 O 2 , we found that H 2 O 2 treatment caused an increase in Nrf2 5ЈUTR activity (Fig. 5A). Reducing La/SSB expression using siRNA resulted in a reduction of H 2 O 2 induced Nrf2 5ЈUTR activity (Fig. 5A). Conversely, overexpression of La/SSB alone was sufficient to induce Nrf2 5ЈUTR activity (Fig. 5B). These results support that La/SSB up-regulated Nrf2 protein translation via interaction with IRES in Nrf2 5ЈUTR.
Subcellular Relocalization of La/SSB under Oxidative Stress-An important question is how oxidants activate La/ SSB, which in turn regulates Nrf2 protein translation. Given the fact that overexpression of La/SSB was sufficient to induce Nrf2 protein, a simplest explanation would be that oxidants induced elevation of La/SSB protein. Western blot analyses of the whole cell lysates did not reveal changes in La/SSB protein levels upon H 2 O 2 treatment, whereas the same set of samples showed a dose dependent elevation of Nrf2 protein (Fig. 6A). Oxidants initiate a cascade of signaling events. Measurements of La/SSB phosphorylation by antibodies against phospho Serine, Threonine, or Tyrosine failed to find positive results (data not shown). Two dimensional Western blot did not show changes in the pattern of La/SSB protein (data not shown), arguing against post-translational modifications. It has been shown that La/SSB is primarily a nuclear protein and can translocate to the cytoplasm (22)(23)(24)(25). When cytosolic proteins were collected for Western blot, a H 2 O 2 dose dependent increase of La/SSB was observed (Fig. 6B). To demonstrate La/SSB translocation upon H 2 O 2 treatment, we measured subcellular localization of La/SSB by immunocytochemical staining and found reduced nuclear La/SSB staining in H 2 O 2 treated cells (Fig. 7). Instead, an increased portion of cells showed perinuclear distribution (Fig. 7). Such perinuclear distribution overlapped with that of ribosomal protein L36a (Fig. 7). To further demonstrate that H 2 O 2 treatment caused subcellular relocalization of La/SSB, we transfected cells with an expression vector for GFP-La/SSB fusion protein. Tracking GFP fluorescence confirmed that H 2 O 2 caused nuclear export and perinuclear redistribution of La/SSB (Fig. 8A). Counting for GFP under a fluorescent microscope indicated that 100 M H 2 O 2 treatment caused threefold increase in the number of cells containing perinuclear La/SSB.
If nuclear export of La/SSB is important for Nrf2 protein translation, inhibition of La/SSB export should abolish Nrf2 protein elevation or Nrf2 5ЈUTR activation by H 2 O 2 . We treated cells with Leptomycin B (LMB), an exportin 1 inhibitor that has been widely used to block nuclear export of proteins (26). Leptomycin B was able to prevent H 2 O 2 from activating Nrf2 5ЈUTR in a dose dependent manner (Fig. 9A). The optimal dose 50 nM of Leptomycin B was able to reduce the level of La/SSB in ribosomal fractions (Fig. 9B). Such treatment reduced the level of Nrf2 protein induction by H 2 O 2 treatment (Fig. 9B).
H 2 O 2 Enhances La/SSB Interaction with Ribosomes-Several lines of evidence suggest H 2 O 2 caused an association of La/SSB with ribosomes: perinuclear distribution, colocalization with L36a, and dose dependent increase of La/SSB protein levels in ribosomal fractions. Ribosomes containing 40S and 60S subunits, have a sedimentation coefficiency of 80S and form polysomes when multiple ribosomes bind to the same strand of mRNA during the process of translation elongation (Fig. 10A). When ribosomes were separated based on their sizes, we found La/SSB was mainly associated with 40S ribosomal fractions, although H 2 O 2 treatment caused an increase of La/SSB in 60/80S ribosomal fractions (Fig. 10B). To test the physical interaction of La/SSB with ribosomes upon H 2 O 2 treatment, we used immunoprecipitation to pull down La/SSB protein and measured its interaction with a representative ribosomal small or large subunit protein, S6 or L36a. We found that H 2 O 2 treatment enhanced La/SSB interaction with the small or large subunit protein S6 or L36a (Fig. 11). This result indicates the physical interaction of La/SSB with ribosomes in cells treated with H 2 O 2 .  DISCUSSION We present here a novel mechanism of de novo Nrf2 protein translation under oxidative stress. A proteomic approach led to the discovery of La/SSB as a binding partner to Nrf2 5ЈUTR. H 2 O 2 induced La/SSB binding to Nrf2 5ЈUTR was confirmed by Far Western and RNA-protein complex immunoprecipitation approaches. We found that oxidants caused nuclear export of La/SSB and such subcellular translocation was important for Nrf2 protein translation. Three lines of evidence indicate increased interaction of La/SSB with ribosomes because of oxidative stress: (1) colocalization of La/SSB with ribosomal protein L36a; (2) H 2 O 2 dose dependent elevation of La/SSB in ribosomal fractions; and (3) binding of La/SSB with L36a or S6 as detected by immunoprecipitation. We have demonstrated that La/SSB played an important role in driving de novo Nrf2 protein translation, because siRNA against La/ SSB prevented H 2 O 2 from inducing Nrf2 protein or activity of Nrf2 5ЈUTR, whereas overexpression of La/SSB was sufficient for inducing Nrf2 protein or activating Nrf2 5ЈUTR. Therefore the interactions of La/SSB with Nrf2 mRNA and with ribosomes are key events underlying oxidant induced de novo Nrf2 protein translation.
Nrf2 5ЈUTR contains a highly conserved 18S rRNA binding site required for internal initiation and a hairpin structure within 103 nt upstream of Nrf2 coding sequence (27). H 2 O 2 treatment was able to activate Nrf2 5ЈUTR as tested by a bicistronic reporter containing the 103 nt sequence (27), consistent with our findings of Nrf2 5ЈUTR activation as reported here and predicted previously (28). The 103 nt Nrf2 5ЈUTR sequence did not interact with the ribosome, nor facilitated the assembly of 48S initiation complex (29). Li et al. (29) postulated that ITAF(s) are required for Nrf2 protein translation through IRES. Indeed we present evidence here that La/SSB acts as an ITAF regulating Nrf2 protein translation under oxidative stress.
La/SSB gene is well conserved in functional domains among mammalian species (30). The protein contains two RNA Recognition Motifs (RRMs) and a nuclear retention element in the C terminus (30,31). These features are important for its functions from tRNA maturation to stabilization and nuclear export of nascent mRNA (30,31). La/SSB was the first ITAF identified for promoting translation of poliovirus (32), and later found to mediate translation of HBV, HCV, HIV, encephalomyocarditis, and giardia viruses (30). For cellular proteins, La/SSB functions as an ITAF for translation of cyclin D1 (33), XIAP (6), BiP (34), and MDM2 (35). In addition to being an ITAF, La/SSB also binds to mRNAs with 5ЈUTR terminal oligopyrimidine track (5Ј-TOP), the products of RNA polymerase III (30). Many of rRNA species and mRNAs encoding certain components of translational machinery contain 5Ј-TOP and therefore are subject of La/SSB regulation (36). In the cytoplasm, binding of La/SSB to 5Ј-TOP targets the mRNA species for translation (31). Although Nrf2 mRNA does not contain 5Ј-TOP, it is possible that La/SSB may indirectly increase Nrf2 protein translation through regulation of genes of translational machinery encoding 5Ј-TOP. Nevertheless, our data are consistent with the finding that La/SSB interacts with ribosomes and promotes recruitment of IRES containing mRNAs onto the ribosomes (37,38).
A number of reports have indicated a role of nuclear export of ITAFs in protein translation. Lewis et al. proposed that ITAFs in the nucleus may bind to their target IRES and sequester the mRNA species from accessible to translational machinery (38). Upon changes in cellular environment, such as viral infection or chemical stress, these ITAFs translocate to the cytoplasm along with their targeted mRNA species. Alternatively, these ITAFs are confined in the nucleus until appropriate signals are received (38). For example, in HeLa cells treated with vincristine, translation of the antiapoptotic protein BAG1 increases through an IRES mediated mechanism (39). PTB and PCBP1 as ITAFs of BAG1 translocate from the nuclei to the cytoplasm upon vincristine treatment. Similarly, hnRNP A1 translocates to the cytoplasm and stimulates IRES mediated translation of fibroblast growth factor 2 in cells challenged with high osmolarity (40,41). ITAF translocation promotes the accessibility to the translational machinery and is essential for translation of the targeted mRNA species.
We reported here that La/SSB protein undergoes subcellular relocalization upon H 2 O 2 treatment. Previous reports show that La/SSB protein is primarily localized in the nucleus and translocates to the cytoplasm upon viral infection (37) or growth factor stimulation (23). The La/SSB protein is subject to phosphorylation at multiple residues, leading to its nuclear export (22)(23)(24)(25). Intine et al. reported that dephosphorylation of Ser366 resulted in cytoplasmic localization of La/SSB (22). Conversely, phosphorylation of Thr301 by AKT in mouse glial progenitor cells promotes its cytoplasmic translocation (23). With immunoprecipitated La/SSB, we failed to observe any changes in phospho-serine, phospho-threonine, or phosphotyrosine (data not shown). Furthermore, inhibiting Akt with LY294002 did not affect H 2 O 2 from inducing Nrf2 either (data not shown). Thus, how La/SSB translocates from the nuclei to the cytoplasm in the H 2 O 2 treated cells remains to be investigated. Two-dimensional Western blot analyses indicate that La/SSB may not undergo post-translational modifications because of H 2 O 2 treatment, suggesting the possibility of a carrier protein in mediating nuclear export of La/SSB.
It has been shown that La/SSB protein is associated with small ribosomal subunits through its binding to 18S rRNA in HeLa cells (42). We have observed an association of La/SSB with 40S ribosomal fractions in HeLa cells (Figs. 10 and 11). Because 40S small subunit together with eIF1/1A/3/2/5 forms the 43S pre-initiation complex that is important for binding to mRNA for translation initiation (43), it is possible that La/SSB binding to Nrf2 mRNA facilitates the landing of 43S Pre-Initiation Complex. An earlier study by Costa-Mattioli et al. (44) supports such a function of La/SSB. Regardless, the physical interaction of La/SSB with ribosomal proteins was detected with immunoprecipitation experiments (Figs. 10 and 11). The presence of La/SSB in 40 to 80S ribosomal fractions (Fig. 11B) supports the role of La/SSB as a key factor for initiating Nrf2 protein translation. Importantly, La/SSB mediated Nrf2 protein translation is not confined to HeLa cells, because similar results were observed in rat primary cultured cardiomyocytes in response to oxidative stress or mouse myocardium in response to ischemic stress (Xu B et al., 2011 manuscript under revision). Since both La/SSB protein and Nrf2 mRNA are ubiquitously expressed in various tissues (30), it is possible that La/SSB mediated de novo Nrf2 protein translation is universal in cells responding to chemical stress. *