Molecular & Cellular Proteomics
Volume 15, Issue 7, July 2016, Pages 2491-2500
Technological Innovation and ResourcesThe Importance of Non-accessible Crosslinks and Solvent Accessible Surface Distance in Modeling Proteins with Restraints From Crosslinking Mass Spectrometry*
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Author contributions: J.M.B. and J.S. designed research; J.M.B. and J.S. performed research; J.M.B. and M.T. analyzed data; J.M.B., J.S., K.T., and M.T. wrote the paper.
- *
This work was supported by BBSRC London Interdisciplinary Doctoral Programme (to J.B) and MRC MR/M019292/1 (to M.T.).
This article contains supplemental material.
- 1
The abbreviations used are:
- SASD
solvent accessible surface distance
- MNXL
matched and non-accessible crosslink score
- NoV
number of violations
- SoVD
sum of violation distances
- XLdb
crosslink database
- XL-MS
crosslinking coupled to mass spectrometry
- BS3
bissulfosuccinimidyl suberate
- DSS
disuccinimidyl suberate
- SEC
size exclusion column
- SCX
strong cation exchange.
© 2016 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.