Galectin-8–mediated selective autophagy protects against seeded tau aggregation

Assembled tau can transfer between cells and seed the aggregation of soluble tau. This process is thought to underlie the amplification and propagation of tau inclusions throughout the brain in neurodegenerative diseases, including Alzheimer's disease. An understanding of the mechanisms involved may provide strategies for limiting assembled tau propagation. Here, we sought to determine how assembled tau seeds gain access to the cytosol and whether this access triggers cellular defenses. We show that tau assemblies enter cells through clathrin-independent endocytosis and escape from damaged endomembranes into the cytosol, where they seed the aggregation of soluble tau. We also found that the danger receptor galectin-8 detects damaged endomembranes and activates autophagy through recruitment of the cargo receptor nuclear dot protein 52 (NDP52). Inhibition of galectin-8– and NDP52-dependent autophagy increased seeded tau aggregation, indicating that autophagy triggered by damaged endomembranes during the entry of assembled tau seeds protects against tau aggregation, in a manner similar to cellular defenses against cytosol-dwelling microorganisms. A second autophagy cargo receptor, p62, then targeted seeded tau aggregates. Our results reveal that by monitoring endomembrane integrity, cells reduce entry of tau seeds into the cytosol and thereby prevent seeded aggregation. The mechanisms described here may help inform the development of therapies aimed at inhibiting the propagation of protein assemblies in neurodegenerative diseases.

Abundant neuronal and glial inclusions composed of filamentous tau protein assemblies define a large number of sporadic human neurodegenerative diseases, collectively known as tauopathies. They include Alzheimer's disease, Pick's disease, tangle-only dementia, chronic traumatic encephalopathy, progressive supranuclear palsy, and corticobasal degeneration. Cases of frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17T), which are caused by mutations in the tau gene, prove that tau dysfunction is sufficient to cause neurodegeneration and dementia (1). Although the molecular species responsible for neurodegeneration are unknown, short filaments are the main species of seed-competent tau in the brains of transgenic mice expressing human P301S tau (2). Unlike Alzheimer's disease, most other tauopathies lack ␤-amyloid deposits. The occurrence of human tauopathies with distinct filament morphologies (3), together with experimental studies (4,5), has led to the idea that multiple molecular conformers of aggregated tau exist. Their definition will need to be structural. As a first step, we recently presented atomic models, as determined by cryo-electron microscopy, for the cores of paired helical and straight tau filaments that were purified from the brain of an individual with Alzheimer's disease (6).
Tau inclusions originate in distinct brain regions, from where they appear to amplify and spread to anatomically connected sites (7). Experimental studies have shown that filamentous assembled tau enters cells and seeds the aggregation of soluble cytosolic tau, followed by release and uptake by neighboring cells (8,9). Although it is known that assembled tau enters the endo-lysosomal system by endocytosis (10 -12), the underlying mechanisms are only incompletely understood and it is not known how they relate to seeded aggregation. In particular, because the endo-lysosomal system does not provide direct access to the cytosol, where seeded aggregation takes place, mechanisms may exist that enable tau seeds to enter the cytosol.
The entry of microorganisms into the mammalian cytosol triggers defense mechanisms that use the autophagy cargo receptors nuclear dot protein 52 (NDP52) 3 (also known as CALCOCO2), p62 (also known as SQSTM1), and optineurin to direct the pathogens to selective macroautophagy (hereafter referred to as autophagy) (13)(14)(15). The danger receptor galectin-8 monitors the integrity of endomembranes by binding exposed luminal glycans of ruptured vesicles, followed by binding to NDP52 and recruitment of the autophagy machinery to the vesicle remnants and their cargo (16). Cargo is then sequestered in autophagosomes, which fuse with lysosomes to mediate degradation (17). It is not known if similar defense mechanisms are active against seeded tau aggregation.
In human tauopathies, the autophagy adaptor protein p62 co-localizes with tau inclusions (18,19). In mice transgenic for human mutant P301S tau, tau inclusions also co-localize with p62 and the autophagosome component LC3 in a ubiquitin-independent manner (20). In these mice, activation of autophagy enhances the clearance of assembled tau and reduces neurodegeneration (20,21). Activation of autophagy also reduces seeded tau aggregation in HEK 293 cells and in induced pluripotent stem cell (iPSC)-derived neuronal cells (22,23). However, the stages at which autophagy restricts seeded aggregation and the mechanisms involved are unknown.
To investigate if assembled tau seeds escape from the endo-lysosomal system and to study downstream effects, we looked at the roles of galectin-8, NDP52, p62, and optineurin. Here we describe how cells protect themselves against seeded tau aggregation by monitoring endomembrane integrity. Following uptake of tau seeds by clathrin-independent endocytosis, galectin-8 detected ruptured endomembranes and recruited NDP52-dependent autophagy. At later time points, seeded tau aggregates were targeted by p62.

Clathrin-independent endocytosis of tau seeds is required for seeded tau aggregation
Monomeric and assembled full-length tau were previously shown to enter cells (12,24). To understand the entry mechanisms, we inhibited the function of proteins involved in endocytosis through the expression of dominant-negative mutants in HEK 293T cells, or pharmacologically in SH-SY5Y cells. We then measured the uptake of fluorescently labeled monomeric and assembled recombinant P301S tau by flow cytometry (Fig.  1, a and b). Extracellular tau was removed with trypsin (12). Transferrin was used as the control.
Expression of a membrane binding-deficient, dominantnegative dynamin mutant lacking the pleckstrin homology domain (⌬PH) or treatment with the dynamin inhibitor Dyngo-4a inhibited the uptake of monomeric and assembled tau, as did expression of GTPase-inactive dominant-negative Cdc42 T17N or treatment with the Cdc42 inhibitor ZCL 278 ( Fig. 1, a and b). In contrast, expression of dominant-negative Rho GTPases Arf6 T27N, RhoA T19N, and Rac1 L17N increased tau uptake, as did expression of dominant-negative endophilin lacking the amphipathic helix (⌬AH) (Fig. 1a). Expression of dominant-negative FCHO2 with a C-terminal red fluorescent protein (RFP) tag, or expression of dominantnegative AP180 C terminus, inhibited the uptake of monomeric tau and of transferrin, whereas assembled tau uptake was increased (Fig. 1a). FCHO and AP180 are required for clathrinmediated endocytosis (25). Treatment with chlorpromazine and monodansylcadaverine inhibited the uptake of monomeric and assembled tau, in addition to inhibiting the uptake of transferrin (Fig. 1b). These molecules inhibit both clathrin-dependent and clathrin-independent endocytosis (26). Treatment with the PI3K inhibitors wortmannin and LY294002 inhibited tau uptake. Transferrin uptake was increased after wortmannin, as observed previously (27), and inhibited by LY294002.
The actin polymerization inhibitors EIPA and latrunculin A inhibited the uptake of monomeric and assembled tau, as previously reported (11,12), whereas transferrin uptake was increased (Fig. 1b). We conclude that tau monomers and tau assemblies enter cells through two distinct pathways, both of which require dynamin, actin, Cdc42, and PI3K. Endocytosis of monomeric tau is clathrin-dependent, whereas that of assembled tau is clathrin-independent.
To determine whether inhibition of assembled tau entry via actin-, Cdc42-, and PI3K-dependent pathways was sufficient to reduce seeded aggregation, 293T cells expressing P301S 1N4R tau were treated with latrunculin A, ZCL 278, and LY294002 during the uptake of assembled recombinant P301S 0N4R tau. Extracellular tau was removed with trypsin (12) and cells were grown in fresh medium without inhibitors for 48 h. Seeded P301S 1N4R tau aggregates were detected by Western blotting of the sarkosyl-insoluble fractions of cells, as described in Ref.
12. They were resolved as two bands of 68 kDa and 59 kDa, corresponding to phosphorylated and nonphosphorylated aggregated P301S 1N4R tau, respectively. P301S 0N4R tau seeds, which run at 52 kDa, were not detected. Treatment with latrunculin A, ZCL 278, and LY294002 reduced seeded tau aggregation (Fig. 1, c and d). These results define the entry pathway of the seed-competent tau species.

Galectin-8 detects the escape of tau from the endo-lysosomal pathway to the cytosol and restricts seeded aggregation
Assembled tau enters cells and trafficks through the endolysosomal system (10). We observed that fluorescently labeled assembled P301S tau seeds co-localized with the lipid membrane-binding dye CellMask in the cytoplasm by 3 h following addition to the culture medium of primary mouse cortical neurons, SH-SY5Y cells, and 293T cells, indicating that tau seeds were contained within intracellular vesicles (Fig. 2, a-c).
We used galectin-8 to investigate if tau seeds gained access to the cytosol from endo-lysosomal vesicles. Galectin-8 detects luminal glycans that become cytosol-exposed upon vesicle rupture (16). Immunofluorescence showed that galectin-8 antibodies detected tau seed-containing vesicles in primary mouse cortical neurons (Fig. 2d) and SH-SY5Y cells (Fig. 2e) 24 h following seed addition, with no vesicular staining in untreated cells. In SH-SY5Y cells, galectin-8 detected tau seed-containing vesicles in 6.3 Ϯ 0.4% of cells (n ϭ 3, Ͼ100 cells/ coverslip). Treatment of HeLa cells expressing YFP-galectin-8 with labeled P301S tau seeds resulted in the formation of galectin-8 -positive, tau seed-containing vesicles in 6.2 Ϯ 0.2% of cells (n ϭ 3, Ͼ100 cells/coverslip) (Fig. 2f). Galectin-8 remained diffuse in untreated cells, suggesting that damage to endo-lysosomal vesicles during tau trafficking was detected by galectin-8. Treatment of cells with filamentous assembled tau from the brains of mice transgenic for human P301S tau, which was previously shown to seed tau aggregation in cells (12), also resulted in the formation of galectin-8 -positive tau seedcontaining vesicles (Fig. S1a). In the majority of cells, galectin-8 detected damage to a single tau seed-containing vesicle, but in some cells multiple vesicles ruptured. Vesicles detected by galectin-8 were also Rab5-positive (Fig. S1b). This shows that  . Arrows indicate co-localization of tau seed-containing vesicles with CellMask/galectin-8. Scale bars, 10 m. g, representative Western blots with HT7 of the total lysate and sarkosyl-insoluble fractions of HeLa cells expressing P301S tau and treated with galectin-8 siRNA, followed by treatment with aggregated P301S tau. GAPDH was used as a loading control. h, densitometric analysis of HT7 blots of the sarkosyl-insoluble fractions from g. The results are the means Ϯ S.E. n ϭ 3; *, p Ͻ 0.05 (Student's t test).

Galectin-8 -mediated autophagy and seeded tau aggregation
during their entry into the cytosol tau seeds rupture endosomal vesicles, which is sensed by galectin-8.
Upon sensing vesicle damage caused by bacteria, galectin-8 functions as a danger receptor to restrict bacterial proliferation in the cytosol (16). To investigate if galectin-8 recruitment to tau seed-containing vesicles restricts seeded aggregation of cytosolic tau, we depleted galectin-8 in HeLa cells expressing P301S tau. Knockdown efficiency was assessed by Western blotting (Fig. S2). Cells treated with galectin-8 siRNAs displayed a 2-fold increase in seeded tau aggregation compared with control siRNA-treated cells (Fig. 2, g and h), illustrating that galectin-8 is a danger receptor that protects against seeded tau aggregation.

Escape of tau seeds from the endo-lysosomal pathway to the cytosol limits seeded aggregation
To further investigate the escape of tau seeds from the endolysosomal system, we expressed GTPase-inactive dominantnegative Rab5 S34N and Rab7 T22N in 293T cells expressing P301S 1N4R tau. These Rab mutants arrest endo-lysosomal trafficking (28). Cells expressing Rab5 S34N and Rab7 T22N showed increased numbers of cells with galectin-8 -positive tau seed-containing vesicles after 24 h compared with control cells (ϳ27 and ϳ19% of Rab5 S34N-and Rab7 T22N-expressing cells, respectively, compared with ϳ6% of control cells) (Fig. 3,  a and b). In the absence of tau seeds, galectin-8 remained diffuse (Fig. S3). Uptake of assembled tau was not significantly different between cells expressing these dominant-negative proteins and control cells, indicating that increased uptake of tau seeds did not account for the increase in galectin-8 -positive vesicles (Fig. 3c). We conclude that arresting endo-lysosomal trafficking increases the escape of tau seeds to the cytosol, possibly by extending the residence time in a susceptible exit compartment, which is detected by galectin-8.
Expression of Rab5 S34N and Rab7 T22N increased seeded tau aggregation after 48 h, by ϳ7-fold and ϳ4-fold, respectively, compared with empty vector controls (Fig. 3, d and e). Bafilomycin A1, which inhibits lysosomal H ϩ ATPases, also increased seeded aggregation compared with untreated cells (Fig. 3, f and g). Total tau was not changed. These results show that arresting endo-lysosomal trafficking of tau seeds increases aggregation. Lipofection of tau seeds, which increases seeded tau aggregation (29), also increased the number of cells with galectin-8 -positive, tau seed-positive structures compared with cells treated with tau seeds in the absence of lipofection, with multiple galectin-8 -positive structures per cell (Fig. S4). We conclude that escape of tau seeds from the endo-lysosomal pathway is limiting for seeded tau aggregation.

NDP52-dependent and p62-dependent autophagy targets tau seeds and seeded tau aggregates, respectively
We asked if cells defend themselves against the entry of assembled tau seeds and seeded tau aggregation through the autophagy cargo receptors NDP52, p62, and optineurin, which were previously shown to defend against bacterial invasion (13)(14)(15). We investigated the targeting of fluorescently labeled assembled tau seeds by these autophagy cargo receptors following uptake in HeLa cells and, subsequently, the targeting of seeded tau aggregates formed after 48 h of growth. We used immunofluorescence with phosphorylation-dependent antitau antibody AT100 to visualize seeded tau aggregates, which are hyperphosphorylated (12), whereas tau seeds remained unphosphorylated (Fig. S5). Tau seeds were detected by NDP52, but not p62, whereas seeded tau aggregates were detected by p62, but not NDP52 (Fig. 4, a and b). Neither tau seeds nor seeded tau aggregates were detected by ubiquitin (Fig.  4, a and b). Similar results were obtained in SH-SY5Y cells (Fig.  4, c and d). Our findings suggest an orderly series of events; tau seeds are targeted by NDP52, whereas subsequently formed seeded tau aggregates are targeted by p62.
To investigate whether targeting by autophagy cargo receptors restricts seeded tau aggregation, we used siRNAs to deplete NDP52, p62, and optineurin in HeLa cells expressing P301S tau. Cells depleted of NDP52 and p62, but not optineurin, displayed a 2-fold increase in seeded tau aggregation compared with control siRNA-treated cells (Fig. 4, e and f). siRNA depletion of essential autophagosome components LC3C, the specific binding partner of NDP52 (30), and FIP200 also increased seeded tau aggregation (Fig. 5, a and b). Amounts of soluble tau were unchanged. Knockdown efficiency was assessed by Western blotting (Fig. S1). In addition to depletion of LC3C and FIP200, we perturbed autophagy pharmacologically following tau seed uptake. Inhibition of autophagy with 3-methyladenine (3-MA) increased seeded tau aggregation in HEK 293T cells expressing P301S tau by more than 2-fold compared with untreated cells (Fig. 5, c and d), whereas activation of autophagy by rapamycin reduced seeded tau aggregation by half (Fig. 5, e and f). Amounts of soluble tau were unchanged. Perturbation of autophagy was detected by monitoring p62 (31) (Fig. 5, e and f). Inhibition of the proteasome with MG132 and treatment with the proteasome activator rolipram had no effect on seeded tau aggregation (Fig. S6). Ubiquitinated protein load and soluble tau were increased after MG132. Taken together, our data show that seeded tau aggregation is restricted by NDP52-dependent autophagy of tau seeds and p62-dependent autophagy of seeded tau aggregates, but not by optineurin-dependent mechanisms.

Discussion
Propagation of protein assemblies requires amplification and spread. It occurs through seeded aggregation in the cytoplasm, followed by the intercellular transfer of assemblies. Here we studied cellular defense mechanisms against assembled tau seed entry and seeded aggregation. Following clathrin-independent endocytosis, galectin-8 detected ruptured endomembranes containing tau seeds and diverted them to autophagy through binding NDP52. This restricted the formation of seeded tau aggregates, which were targeted by p62. These findings show that monitoring endomembrane integrity protects against the entry of assembled tau seeds and subsequent seeded tau aggregation in a manner similar to cell-autonomous immunity defending the cytosol against pathogen entry (Fig. 7).
We show that assembled full-length tau enters cells by clathrin-independent endocytosis, which requires actin, PI3K and Cdc42, confirming and extending previous work (10 -12). This defines the uptake pathway of tau seeds, because actin-, PI3K-, and Cdc42-dependent endocytosis was required for seeded aggregation. The clathrin-independent uptake of aggregated tau also required dynamin, confirming that dynamindependence is not limited to clathrin-mediated endocytosis (25). It appears likely that assembled tau is heterogeneous and that only a fraction is taken up into cells and seeds aggregation (2). By contrast, monomeric tau is taken up by actin-dependent, clathrin-mediated endocytosis. In view of these different mechanisms of endocytosis, it is highly unlikely that our monomeric tau preparations contained some assembled tau, as previously suggested (32). Dependence on PI3K and Cdc42 may be linked to the requirement for actin, because these proteins regulate actin polymerization at the plasma membrane (33,34). Alternatively, they may be required for the recycling of a receptor. Assembled tau induces endocytosis (11), suggesting that cellsurface receptors for tau may exist.
Increased tau and transferrin uptake following expression of dominant-negative mutant proteins (C-terminal-tagged FCHO, endophilin A2, RhoA T19N, Rac1 T17N, and Arf6 T27N) and small molecule inhibitors (EIPA, wortmannin, and latrunculin A), respectively, may reflect up-regulation of other endocytic pathways. Inhibition of some endocytic pathways can lead to the up-regulation of others (26).
Galectin-8 detects the rupture of endomembranes caused by tau seeds. This shows how assembled tau that is initially contained within vesicles can seed the aggregation of soluble tau in the cytosol. The mechanisms leading to the rupture of tau seed-containing endomembranes are unknown, but may involve assembled tau-dependent membrane disruption. Tau assemblies are hydrophobic and can impair the integrity of artificial membranes (35,36). Recombinant, heparin-assembled tau also induces the formation of galectin-3 puncta in cells (36), as does the addition of cell lysates (37), which may be dependent on the presence of assembled tau in the lysates.
Escape of tau seeds into the cytosol represents a bottleneck for seeded aggregation, because most seeds were not detected by galectin-8 and because increasing escape through stalling endo-lysosomal trafficking elevated seeded assembly. Membrane barriers, in addition to galectin-8-and NDP52-dependent autophagy, therefore represent defense mechanisms against the entry of tau seeds into the cytosol. This confirms the extent to which compartmentalization of the cytosol protects cells from extracellular threats (38). Escape of tau seeds occurred mostly from endosomes, because galectin-8 detected tau seed-containing vesicles that were Rab-positive, despite its ability to detect both endosomes and lysosomes (16). Expression of dominant-negative Rab5 led to a greater increase in seeded tau aggregation than that of dominant-negative Rab7. Assembled ␣-synuclein may also access the cytosol through similar mechanisms, because it enters the endo-lysosomal system (39) and because galectin-3 is recruited to vesicles following their uptake (36,40).
We have shown that cells defend themselves against seeded tau aggregation using two autophagy cargo receptors, NDP52 and p62, which act at different stages. The initial response to the escape of assembled tau seeds to the cytosol requires a galectin-8 -and NDP52-dependent pathway, whereas a p62-dependent pathway targets seeded tau aggregates that form at later time points. These findings establish that galectin-8 can direct autophagy to cargo other than microorganisms and that seeded tau aggregation is primarily restricted by autophagy, rather than the proteasome. The autophagy pathways described here are ubiquitin-independent, consistent with studies in human tauopathy brains (41)(42)(43) and the central nervous system of mice transgenic for human mutant P301S tau (20). A p62-dependent pathway may be common to protein assemblies and be dependent on the PB1 domain of p62,

Galectin-8 -mediated autophagy and seeded tau aggregation
because it is required for the ubiquitin-independent targeting of assembled SOD1 and an aggregation-prone mutant of the transcription factor STAT5A (44,45). Unlike tau seeds assembledfromrecombinantprotein,seededtauaggregateswerephosphorylated, indicating that tau phosphorylation is a consequence of seeded aggregation. It remains to be seen if seeded tau aggregation is influenced by other post-translational modifications.
In conclusion, we have shown that membrane barriers and mechanisms that monitor their integrity protect cells against seeded tau aggregation, in addition to colonization by microorganisms. Our findings reveal the extent to which surveying the integrity of endomembranes protects cells against seeded aggregation.

Experimental procedures
Cell culture HEK 293T and HeLa cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with GlutaMAX and 10% fetal  (a and b) and SH-SY5Y cells (c and d) expressing P301S tau treated with DyLight-labeled aggregated P301S tau (red) for 3 h, followed by 24-h growth and immunostaining with anti-NDP52, anti-p62, and anti-ubiquitin (green) (a and b) or treated with aggregated recombinant P301S tau for 3 h, followed by 48-h growth and immunostaining with AT100 (green) and anti-NDP52, anti-p62, and anti-ubiquitin (red) (c and d). Nuclei were visualized with DAPI (blue). Arrows indicate co-localization of tau seed-containing vesicles with NDP52 and seeded tau aggregates with p62. Scale bars, 10 m. e, representative Western blots with HT7 of the total lysate and sarkosyl-soluble and sarkosyl-insoluble fractions of HeLa cells expressing P301S tau and treated with NDP52, p62, and optineurin siRNAs, followed by treatment with aggregated P301S tau. GAPDH was used as a loading control. f, densitometric analysis of HT7 blots of the sarkosyl-insoluble fractions from e. The results are the means Ϯ S.E. n ϭ 4; **, p Ͻ 0.01 (ANOVA). Figure 5. Autophagy restricts seeded tau aggregation. a, representative Western blots with HT7 of the sarkosyl-insoluble fractions of HeLa cells expressing P301S tau and treated with LC3 and FIP200 siRNAs, followed by treatment with aggregated P301S tau. GAPDH was used as a loading control. b, densitometric analysis of HT7 blots of the sarkosyl-insoluble fractions from a. c, representative Western blots with HT7 of the total lysate, sarkosyl-soluble and sarkosylinsoluble fractions and anti-p62 of total lysates of 293T cells expressing P301S 1N4R tau treated with aggregated P301S tau for 3 h, followed by 48-h incubation with 3-methyladenine. d, densitometric analysis of HT7 blots of the sarkosyl-soluble (squares) and sarkosyl-insoluble (triangles) fractions from c. e, representative Western blots with HT7 of the total lysate, sarkosyl-soluble and sarkosyl-insoluble fractions, and anti-p62 of total lysates of 293T cells expressing P301S 1N4R tau treated with aggregated P301S tau for 3 h, followed by 48-h incubation with rapamycin. f, densitometric analysis of HT7 blots of the sarkosyl-soluble (squares) and sarkosyl-insoluble (triangles) fractions from e. In a, c, and e, GAPDH was used as a loading control. In b, d, and f, the results are the means Ϯ S.E. n ϭ 3; **, p Ͻ 0.01; ***, p Ͻ 0.001; ****, p Ͻ 0.0001 (ANOVA).

Galectin-8 -mediated autophagy and seeded tau aggregation
calf serum (FCS). SH-SY5Y cells were cultured in minimal essential medium (MEM) and Ham's F-12 (1:1), supplemented with GlutaMAX, nonessential amino acids, and 10% FCS. Cell lines were passaged by incubation with 0.025% trypsin/EDTA for 5 min at 37°C. They were validated and obtained from the European Collection of Authenticated Cell Cultures (ECACC). Primary mouse cortical neurons were prepared from E18.5 C57BL/6J embryos as described (46) and used for experiments 14 days after plating on poly-L-lysine-coated coverslips or dishes. Cell culture reagents were from Gibco.

Purification, assembly, and labeling of tau seeds
Recombinant human full-length P301S 0N4R tau was expressed and purified as described (12). Aliquots were frozen in liquid nitrogen and stored at Ϫ20°C. Prior to an experiment, recombinant tau was centrifuged at 100,000 ϫ g at 4°C for 1 h and the supernatants used immediately. Recombinant tau (60 M) was incubated with heparin (15 M) (Sigma) in 30 mM

Galectin-8 -mediated autophagy and seeded tau aggregation
MOPS, pH 7.2, with 100 M AEBSF at 37°C for 72 h without shaking or sonication, as described (47), to produce filamentous assemblies, as characterized in Ref. 12. Recombinant tau was covalently labeled with DyLight dyes (Thermo Fisher) using the succinimidyl ester amine reaction as described (12). Sarkosylinsoluble filamentous assembled tau from the brains of aged mice transgenic for human P301S tau displaying a neurodegenerative phenotype (48) was extracted as described (12) and used immediately.

Galectin-8 -mediated autophagy and seeded tau aggregation Statistical analysis
All results are expressed as means Ϯ S.E., and n values refer to the number of independent experiments. Statistical comparisons between groups were performed using the two-tailed Student's t test or by analysis of variance (ANOVA), as detailed in the figure legends. Dunnett's test was used to control for multiple comparisons. GraphPad Prism 7 software was used to perform analyses.
Author contributions-All authors contributed to designing the experiments. B.F. and J.N. conducted the experiments and data analyses. B.F., J. N., H. M., F.R., and M.G. wrote the manuscript.