Journal of Biological Chemistry
EnzymologyX-ray–induced photoreduction of heme metal centers rapidly induces active-site perturbations in a protein-independent manner
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Author contributions—V. P., K. D.-C., and S. H. conceptualization; V. P., J. H. B., H. M., D. S., T. G., and S. H. data curation; V. P., J. H. B., H. M., D. S., T. G., and S. H. formal analysis; V. P. and S. H. supervision; V. P., J. H. B., and S. H. validation; V. P. and S. H. visualization; V. P., J. H. B., K. D.-C., and S. H. methodology; V. P.,J. H. B., K. D.-C., and S. H. writing-original draft; V. P. and S. H. project administration; V. P., J. H. B., C. O., K. D.-C., and S. H. writing-review and editing; J. H. B. software; C. O. and K. D.-C. resources; C. O., K. D.-C., and S. H. funding acquisition.
Funding and additional information—This project was supported by the Austrian Science Fund, FWF (Doctoral Program BioToP - Molecular Technology of Proteins (W1224) and the project P29099), Diamond Light Source Proposal MX19036, ESRF Proposal MX1998, and the iNEXT program (PID: 4482). The work of K. D.-C. was supported by Austrian Science Fund (FWF) Projects I525, I1593, P22276, P19060, and W1221; Wellcome Trust Collaborative Award 201543/Z/16/Z; by COST action BM1405—Non-globular proteins–from sequence to structure, function, and application in molecular physiopathology (NGP-NET); WWTF (Vienna Science and Technology Fund) Chemical Biology Project LS17-008; the Austrian Federal Ministry of Science, Research, and Economy; and the National Foundation for Research, Technology, and Development through funding the Christian Doppler Laboratory for High-Content Structural Biology and Biotechnology, Austria.
Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.
Present address for John H. Beale: Paul Scherrer Institute, Villigen, Switzerland.
Abbreviations—The abbreviations used are:
- Gy
gray(s)
- AvTsdA
thiosulfate dehydrogenase from Allochromatium vinosum
- CCld
chlorite dismutase from Cyanothece sp. PCC7425
- hhMb
horse heart myoglobin
- KpDyP
dye-decolorizing peroxidase from Klebsiella pneumoniae
- LmChdC
coproheme decarboxylase from Listeria monocytogenes
- HS
high-spin
- LS
low-spin
- NdCld
chlorite dismutase from Nitrospira defluvii
- PDB
Protein Data Bank
- XFEL
X-ray free-electron laser
- DLS
Diamond Light Source
- wp
water position
- BisTris
2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol
- Bicine
N,N-bis(2-hydroxyethyl)glycine.