Journal of Biological Chemistry
Molecular Basis of Cell and Developmental BiologyFBP17 Mediates a Common Molecular Step in the Formation of Podosomes and Phagocytic Cups in Macrophages*
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The abbreviations used are: M-CSF-1, macrophage-colony stimulating factor-1; FBP17, formin-binding protein 17; WAS, Wiskott-Aldrich syndrome; WASP, Wiskott-Aldrich syndrome protein; N-WASP, neuronal WASP; WIP, WASP interacting-protein; EFC domain, extended FER-CIP4 homology domain; F-BAR domain, FER-CIP4 homology and Bin-amphiphysin-Rvs domain; PMA, phorbol 12-myristate 13-acetate; GFP, green fluorescence protein; siRNA, short interfering RNA; FITC, fluorescein isothiocyanate; PDZ-GEF, PDZ-guanine nucleotide exchange factor; HEK293 cells, human embryonic kidney 293 cells; HA, hemagglutinin; SH3, src homology 3 domain; dSH3, SH3 domain deletion; GST, glutathione S-transferase;, PI(4,5)P2, phosphatidylinositol 4,5-bisphosphate; siFBP, siRNA for FBP17; siC, scrambled control siRNA.
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This work was supported, in whole or in part, by National Institutes of Health Grant R01HD042752 (to S. T.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
The on-line version of this article (available at http://www.jbc.org) contains six supplemental figures.