Review
A Human Proteome Detection and Quantitation Project*

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The lack of sensitive, specific, multiplexable assays for most human proteins is the major technical barrier impeding development of candidate biomarkers into clinically useful tests. Recent progress in mass spectrometry-based assays for proteotypic peptides, particularly those with specific affinity peptide enrichment, offers a systematic and economical path to comprehensive quantitative coverage of the human proteome. A complete suite of assays, e.g. two peptides from the protein product of each of the ∼20,500 human genes (here termed the human Proteome Detection and Quantitation project), would enable rapid and systematic verification of candidate biomarkers and lay a quantitative foundation for subsequent efforts to define the larger universe of splice variants, post-translational modifications, protein-protein interactions, and tissue localization.

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Published, MCP Papers in Press, January 7, 2009, DOI 10.1074/mcp.R800015-MCP200

*

This work was supported, in whole or in part, by National Institutes of Health Grant 1U24 CA126476-02 (to N. L. A., T. W. P., C. H. B., A. G. P., and S. A. C.) from the National Cancer Institute as part of the NCI Clinical Proteomic Technologies Initiative.