ENZYME CATALYSIS AND REGULATION
Human Alveolar Macrophages and Granulocyte-macrophage Colony-stimulating Factor-induced Monocyte-derived Macrophages Are Resistant to H2O2 via Their High Basal and Inducible Levels of Catalase Activity*

https://doi.org/10.1074/jbc.M102081200Get rights and content
Under a Creative Commons license
open access

Human alveolar macrophages (A-MΦ) and macrophages (MΦ) generated from human monocytes under the influence of granulocyte-macrophage colony-stimulating factors (GM-MΦ) express high levels of catalase activity and are highly resistant to H2O2. In contrast, MΦ generated from monocytes by macrophage colony-stimulating factors (M-MΦ) express low catalase activity and are about 50-fold more sensitive to H2O2 than GM-MΦ or A-MΦ. Both A-MΦ and GM-MΦ but not M-MΦ can induce catalase expression in both protein and mRNA levels when stimulated with H2O2 or zymosan. M-MΦ but not GM-MΦ produce a large amount of H2O2 in response to zymosan or heat-killed Staphylococcus aureus. These findings indicate that GM-MΦ and A-MΦ but not M-MΦ are strong scavengers of H2O2 via the high basal level of catalase activity and a marked ability of catalase induction and that catalase activity of MΦ is regulated by colony-stimulating factors during differentiation.

Cited by (0)

Published, JBC Papers in Press, April 19, 2001, DOI 10.1074/jbc.M102081200

*

This study was supported in part by grants from the Japan Health Science Foundation and the Ministry of Health and Welfare of Japan (to K. S. A.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

© 2001 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.