Abstract
Water-soaking and then necrosis occurred after 3–5 days in leaves of Phaseolus vulgaris cv. Epicure inoculated with race adhi of Uromyces appendiculatus. Changes in the appearance of host plasmalemmae and cytoplasm around haustoria did not occur until 56 h and preceded deterioration in adjacent uninfected cells. The penetrated cells remained alive until after 72 h when necrosis had occurred one or a few cells ahead of the intercellular hyphae and in rings around the infection sites. The number of necrotic cells in the rings increased and reached a maximum 7 days after inoculation when growth ceased having given rise to uredinial initials at some sites.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bailey, J.A. and Ingham, J.L. (1971)—Phaseollin accumulation in bean (Phaseolus vulgaris) in response to infection by tobacco necrosis virus and the rust Uromyces appendiculatus. Physiological Plant Pathology 1: 451–456.
Bailey, J.A., Rowell, P.M. and Arnold, G.M. (1980)—The temporal relationship between host cell death, phytoalexin accumulation and fungal inhibition during hypersensitive reactions of Phaseolus vulgaris to Colletotrichum lindemuthianum. Physiological Plant Pathology 17: 329–339.
Ballantyne, B. (1978)—The genetic bases of resistance to rust, caused by Uromyces appendiculatus in bean (Phaseolus vulgaris). PhD Thesis, The Univeristy of Sydney.
Buonario, R., DellaTorre, G. and Montalbini, P. (1987)— Soluble superoxide dismutase (SOD) in susceptible and resistant host-parasite complexes of Phaseolus vulgaris and Uromyces phaseoli. Physiological and Molecular Plant Pathology 31: 173–184.
Bushnell, W.R. (1982)—Hypersensitivity in rusts and powdery mildews. In Plant Infection: The Physiological and Biochemical Basis, (Eds Y. Asada, W.R. Bushnell, S. Ouchi and C.P. Vance), pp. 97–116. Japan Scientific Societies Press: Tokyo and Springer-Verlag: Berlin, Heidleberg and New York.
Chong, J., Harder, D.E. and Rohringer, R. (1981)— Ontogeny of mono- and dikaryotic rust haustoria; cytochemical and ultrastructural studies. Phyfopathology 71: 975–989.
Chong, J., Harder, D.E. and Rohringer, R. (1986)— Cytochemical studies on Puccinia graminis f.sp. tritici in a compatible wheat host. II. Haustorium mother cell walls at the host cell penetration site, haustorial walls, and the extrahaustorial matrix. Canadian Journal of Botany 64: 2561–2575.
Crute, I.R., De Wit, P.J.G.M. and Wade, M. (1985)— Mechanisms by which genetically controlled resistance and virulence influence host colonization by fungal and bacterial parasites. In Mechanisms of Resistance to Plant Diseases (Ed R.S.S. Fraser), pp. 107–309. Martinus Nijhoff/Dr W. Junk: Dordrecht, Boston and Lancaster.
Del Rio, L.A., Lyon, D.S., Olah, I., Glick, B. and Salin, M.L. (1983)—Immunocytochemical evidence for a peroxisomal localization of manganese superoxide dismutase in leaf protoplasts from a higher plant. Planta 158: 216–224.
Doke, N. (1983)—Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Physiological Plant Pathology 23: 345–357.
Elnaghy, M.A. and Heitefuss, R. (1976)—Permeability changes and production of antifungal compounds in Phaseolus vulgaris infected with Uromyces phaseoli. II. Role of phytoalexins. Physiological Plant Pathology 8: 269–277.
Harder, D.E., Rohringer, R., Samborski, D.J., Kim, W.K. and Chong, J. (1979a)—Electron microscopy of susceptible and resistant near-isogenic (sr6/Sr6) lines of wheat infected by Puccinia graminis tritici. II. Expression of incompatibility in mesophyll and epidermal cells and the effect of temperature on host-parasite interactions in these cells. Canadian Journal of Botany 57: 2617–2625.
Harder, D.E., Rohringer, R., Samborski, D.J., Kim, W.K. and Chong, J. (1979b)—Electron microscopy of susceptible and resistant near-isogenic (sr6/Sr6) lines of wheat infected by Puccinia graminis tritici. Ill. Ultrastructure of incompatible interactions. Canadian Journal of Botany 57: 2626–2634.
Hardwick, N.V., Greenwood, A.D. and Wood, R.K.S. (1971)—The fine structure of the haustorium of Uromyces appendiculatus in Phaseolus vulgaris. Canadian Journal of Botany 49: 383–390.
Heath, M.C. (1972)—Ultrastructure of host and nonhost reactions to cowpea rust. Phytopathology 62: 27–38.
Heath, M.C. (1981)—A generalized concept of hostparasite specificity. Phytopathology 71: 1121–1123.
Heath, M.C. (1982)—Host defense mechanisms against infection by rust fungi. In The Rust Fungi (Eds K.J. Scott and A.K. Chakravorty), pp. 223–245. Academic Press: London.
Heath, M.C. and Heath, I.B. (1971)—Ultrastructure of an immune and a susceptible reaction of cowpea leaves to rust infections. Physiological Plant Pathology 1: 277–287.
Ingram, D.S. (1978)—Cell death and resistance to biotrophs. Annals of Applied Biology 89: 291–295.
Ingram, D.S. (1982)—A structural view of active defence. In Active Defense Mechanisms in Plants (Ed R.K.S. Wood), pp. 19–38. Plenum Press: New York and London.
Jones, D.R. and Deverall, B.J. (1977)—The effect of the Lr20 resistance gene in wheat on the development of leaf rust, Puccinia recondita. Physiological Plant Pathology 10: 275–284.
Jones, D.R. and Deverall, B.J. (1978)—The use of leaf transplants to study the cause of hypersensitivity of leaf rust, Puccinia recondita, in wheat carrying the Lr20 gene. Physiological Plant Pathology 12: 311 -319.
Kiraly, Z., Barna, B. and Ersek, T. (1972)—Hypersensitivity as a consequence, not the cause of plant resistance to infection. Nature 239: 456–458.
Kiraly, Z., El Hamady, M. and Pozsar, B.I. (1967)— Increased cytokinin activity of rust-infected bean and broad bean leaves. Phytopathology 57: 93–94.
Littlefield, L.J. and Heath, M.C. (1979)—Ultrastructure of Rust Fungi. Academic Press: New York.
Mendgen, K. (1973)—Feinbau der lnfektionsstruckturen von Uromyces phaseoli. Phytopathologische Zeitschrift 78: 109–120.
Muller, L.Y., Rijkenberg, F.H.T. and Truter, S.J. (1974)— Ultrastructure of the uredial stage of Uromyces appendiculatus. Phytophylactica 6: 73–104.
Novacky, A. (1972)—Suppression of the bacterially induced hypersensitive reaction by cytokinins. Physiological Plant Pathology 2: 101–104.
Sandalio, L.M. and Del Rio, L.A. (1987)—Localization of superoxide dismutase in glyoxysomes from Citrullus vulgaris. Functional implications in cellular metabolism. Journal of Plant Physiology 127: 395–409.
Sandalio, L.M., Fernandez, V.M., Ruperez, F.L. and Del Rio, L.A. (1988)—Superoxide free radicals are produced in glyoxysomes. Plant Physiology 87: 1–4.
Stavely, J.R., Freytag, G.F., Steadman, J.R. and Schwartz, H.F. (1983)—The 1983 bean rust workshop. Annual Report Bean Improvement Cooperative 26: iv-vi.
Tolbert, N.E. (1980)—Microbodies — peroxisomes and glyoxysomes. In The Biochemistry of Plants, a Comprehensive Treatise (Eds P.K. Stumpf and E.E. Conn), pp. 359–388. Academic Press: New York.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ye, Xs., Deverall, B.J. Optical and electron microscopy of a slowly developing major incompatibility in bean rust infections. Australasian Plant Pathology 18, 71–76 (1989). https://doi.org/10.1071/APP9890071
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1071/APP9890071