Abstract
Tilletia indica, the cause of Karnal bunt of wheat, is the target of strict quarantine regulations by most wheatgrowing countries and its presence raises trade barriers to wheat exports. The international diagnostic protocol currently used in Australia involves the tentative identification of the spores based on morphology followed by germination of the spores and a molecular protocol to confirm the identity. This paper reports on the development of an enhanced protocol by the incorporation of a highly sensitive one-tube molecular assay for T. indica and related Tilletia species found as grain contaminants. The enhanced protocol reduces the amount of time and labour required in microscopy and bypasses the germination of Tilletia spores in the processing of samples. An economic analysis has been performed to compare the economic costs of the current and the enhanced protocol and their relative performance in different scenarios. The results indicated that the current protocol would be more economical in the monitoring and identification of T. indica in current infested areas. However, the enhanced protocol is significantly more economical and sensitive for use in an exotic pest incursion scenario or a survey to demonstrate area of freedom. The enhanced protocol also reduces the elapsed time before a definitive identification can be achieved.
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References
Allen TW, Maples HW, Workneh F, Stein JM, Rush CM (2008) Distribution and recovery of Tilletia indica teliospores from regulated wheat fields in Texas. Plant Disease 92, 344–350. doi:10.1094/PDIS-92-3-0344
Babadoost M, Mathre DE, Johnston RH, Bonde MR (2004) Survival of teliospores of Tilletia indica in soil. Plant Disease 88, 56–62. doi:10.1094/PDIS.2004.88.1.56
Bonde MR, Berner DK, Nester SE, Peterson GJ, Olsen MW, Cunfer BM, Sim T (2004) Survival of Tilletia indica teliospores in different soils. Plant Disease 88, 316–324. doi:10.1094/PDIS.2004.88.4.316
Brennan JP, Warham EJ (1990) Economic losses from Karnal bunt of wheat in Mexico. CIMMYT Economics Working Paper 90/02, International Maize and Wheat Improvement Centre, Mexico, DF.
Brennan JP, Warham EJ, Byerlee D, Hernandez-Estrada J (1992) Evaluating the economic impact of quality reducing, seed-borne diseases: lessons from Karnal bunt of wheat. Agricultural Economics 6, 345–352. doi:10.1016/0169-5150(92)90010-V
Brown A, Bragatheswaran G, Foster M, To H, Berry P, Lawrance L (2009) Australian Crop Report No. 151. ABAREproject 1076, Australian Bureau of Agricultural and Resource Economics, Canberra, ACT.
Frederick RD, Snyder KE, Tooley PW, Berthier-Schaad Y, Peterson GL, Bonde MR, Schaad NW, Knorr DA (2000) Identification and differentiation of Tilletia indica and T. walkeri using the polymerase chain reaction. Phytopathology 90, 951–960. doi:10.1094/PHYTO.2000. 90.9.951
Fuentes-Davila G (1996) Karnal bunt. In ‘Bunt and Smut diseases of wheat: concepts and methods of disease management’. (Eds RD Wilcoxson, EE Saari) pp. 26–32. (CIMMYT: Mexico, DF)
Inman AJ, Hughes KJD, Bowyer RJ (2003) ‘EU recommended protocol for the diagnosis of a quarantine pathogen, Tilletia indica.’ (Central Science Laboratory: New York, UK) Available at http://www.fera.defra.gov. uk/plants/plantHealth/pestsDiseases/documents/protocols/tipro.pdf [last accessed 3 March 2010]
Krishna A, Singh RA (1983) Longevity of teliospores of Neovossia indica causing Karnal bunt of wheat. Indian Journal of Mycology and Plant Pathology 13, 97–98.
Murray GM, Brennan JP (1998) The risk to Australia from Tilletia indica, the cause of Karnal bunt of wheat. Australasian Plant Pathology 27, 212–225. doi:10.1071/AP98024
Pascoe IG, Priest MJ, Shivas RG, Cunnington JH (2005) Ustilospores of Tilletia ehrhartae, a smut of Ehrharta calycina, are common contaminants of Australian wheat grain, and a potential source of confusion with Tilletia indica, the cause of Karnal bunt of wheat. Plant Pathology 54, 161–168. doi:10.1111/j.1365-3059.2005.01145.x
Rush CM, Stein JM, Bowden RL, Riemenschneider R, Boratynski T, Royer MH (2005) Status of Karnal bunt of wheat in the United States 1996 to 2004. Plant Disease 89, 212–223. doi:10.1094/PD-89-0212
Stansbury CD, McKirdy SJ (2002) Forecasting climate suitability for Karnal bunt of wheat: a comparison of two meteorological methods. Australasian Plant Pathology 31, 81–92. doi:10.1071/AP01080
Stansbury CD, McKirdy SJ, Diggle AJ, Riley IT (2002) Modelling the risk of entry, establishment, spread, containment, and economic impact of Tilletia indica, the cause of Karnal bunt of wheat, using an Australian context. Phytopathology 92, 321–331. doi:10.1094/PHYTO.2002. 92.3.321
Stein JM, Maples HW, Rush CM (2005) Epidemiology of Tilletia indica teliospores in regulated wheat fields in Texas. Plant Disease 89, 828–833. doi:10.1094/PD-89-0828
Tan M-K, Wright D (2009) Enhancing the detection of Tilletia indica, the cause of Karnal bunt. Final Report CRC20004, Cooperative Research Centre for National Plant Biosecurity, Canberra, ACT.
Tan M-K, Ghalayini A, Sharma I, Yi J, Shivas R, Priest M, Wright D (2009) A one-tube fluorescent assay for the quarantine detection and identification of Tilletia indica and other grass bunts in wheat. Australasian Plant Pathology 38, 101–109. doi:10.1071/AP08088
Wright D, Murray GM, Tan M-K (2003) National Diagnostic Protocol for the identification of Tilletia indica, the cause of Karnal Bunt. Department of Agriculture, Western Australia, Orana.
Ykema RE, Floyd JP, Palm ME, Peterson GL (1996) First report of Karnal bunt of wheat in the United States (Disease Note). Plant Disease 80, 1207.
Zhang Z, Lange L, Mathur SB (1984) Teliospore survival and plant quarantine significance of Tilletia indica (causal agent of Karnal bunt) particularly in relation to China. EPPO Bulletin 14, 119–128. doi:10.1111/j.1365-2338.1984.tb01858.x
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Tan, MK., Brennan, J.P., Wright, D. et al. An enhanced protocol for the quarantine detection of Tilletia indica and economic comparison with the current standard. Australasian Plant Pathol. 39, 334–342 (2010). https://doi.org/10.1071/AP10003
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DOI: https://doi.org/10.1071/AP10003